Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells

Yu, Fangcang; Cui, Yazhou; Zhou, Xiaoyan; Zhang, Xiumei; Han, Jinxiang

doi:10.5582/bst.2011.v5.2.46

Cited by 54 publications

(47 citation statements)

References 19 publications

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“…In this environment fibroblasts exhibited high levels of alkaline phosphatase and mineral deposition, but more interestingly, microRNA expression profiles revealed a significant down-regulation of a group of microRNAs known to negatively interfere with genes associated with osteogenic differentiation (e.g., BMP2, OC, Runx2). In the light of these data, it has been hypothesized that osteoclasts might induce the osteogenic differentiation of fibroblasts in vitro and that miRNA may play an important role in the regulation of cell–cell interactions between osteoclasts and fibroblasts (Yu et al, 2011). …”

Section: Role Of Mesenchymal Cells In Soft Connective Tissue Calcificmentioning

confidence: 99%

Fibroblast involvement in soft connective tissue calcification

Ronchetti¹,

Boraldi²,

Annovi³

et al. 2013

Front. Genet.

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Soft connective tissue calcification is not a passive process, but the consequence of metabolic changes of local mesenchymal cells that, depending on both genetic and environmental factors, alter the balance between pro- and anti-calcifying pathways. While the role of smooth muscle cells and pericytes in ectopic calcifications has been widely investigated, the involvement of fibroblasts is still elusive. Fibroblasts isolated from the dermis of pseudoxanthoma elasticum (PXE) patients and of patients exhibiting PXE-like clinical and histopathological findings offer an attractive model to investigate the mechanisms leading to the precipitation of mineral deposits within elastic fibers and to explore the influence of the genetic background and of the extracellular environment on fibroblast-associated calcifications, thus improving the knowledge on the role of mesenchymal cells on pathologic mineralization.

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Section: Role Of Mesenchymal Cells In Soft Connective Tissue Calcificmentioning

confidence: 99%

Fibroblast involvement in soft connective tissue calcification

Ronchetti¹,

Boraldi²,

Annovi³

et al. 2013

Front. Genet.

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“…Interestingly, it has been suggested that not only osteoblasts regulate osteoclast activity, but osteoclasts may also, in turn, influence osteoblast differentiation. Several mechanisms may be involved, including changes in the expression levels of some miRNAs [97]. …”

Section: Epigenetic Changes During Osteoclastogenesis: Control Of Remmentioning

confidence: 99%

Do Epigenetic Marks Govern Bone Mass and Homeostasis?

Delgado‐Calle

Garmilla

Riancho

2012

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Bone is a specialized connective tissue with a calcified extracellular matrix in which cells are embedded. Besides providing the internal support of the body and protection for vital organs, bone also has several important metabolic functions, especially in mineral homeostasis. Far from being a passive tissue, it is continuously being resorbed and formed again throughout life, by a process known as bone remodeling.Bone development and remodeling are influenced by many factors, some of which may be modifiable in the early steps of life. Several studies have shown that environmental factors in uterus and in infancy may modify the skeletal growth pattern, influencing the risk of bone disease in later life. On the other hand, bone remodeling is a highly orchestrated multicellular process that requires the sequential and balanced events of osteoclast-mediated bone resorption and osteoblast-mediated bone formation. These processes are accompanied by specific gene expression patterns which are responsible for the differentiation of the mesenchymal and hematopoietic precursors of osteoblasts and osteoclasts, respectively, and the activity of differentiated bone cells. This review summarizes the current understanding of how epigenetic mechanisms influence these processes and their possible role in common skeletal diseases.

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“…miR-320a was previously shown to be involved in the development and invasiveness of several malignancies including colorectal cancer, nasopharyngeal carcinoma, hepatocellular carcinoma and breast cancer [26][27][28][29][30]. However, its role in MSC differentiation or osteogenic lineage determination has not been analyzed in detail, although it was identified among several miRNAs showing differential expression in association with osteogenic differentiation of human ligament fibroblasts [31].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10

Huang¹,

Meng

Liu

et al. 2016

Cell Physiol Biochem

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Background/Aims: Human bone marrow-derived mesenchymal stem cells (hMSCs) are a promising cell source for bone engineering owing to their high potential to differentiate into osteoblasts. The bone morphogenetic protein-inducible gene homeobox a10 (HOXA10) is a critical regulator of osteogenesis. The objective of the present study was to identify microR-NAs (miRNAs) targeting HOXA10 and examine the effects on the osteogenic differentiation of hMSCs. Methods: Based on in silico analysis, HOXA10-targeting miRNAs were selected and their regulatory roles in osteoblast differentiation were investigated. Results: Six HOXA10-targeting miRNAs were identifIed by computational analysis, of which miR-320a was selected for further analysis because it was downregulated during osteogenic induction. Overexpression of miR-320a downregulated HOXA10 and significantly inhibited osteogenesis in hMSCs, as determined by the downregulation of the osteogenic markers Runx2, ALP, and OC and the inhibition of ALP activity and matrix mineralization, whereas miR-320a inhibition had the opposite effects. Furthermore, ectopic expression of HOXA10 (not including 3′-UTR) rescued the effects of miR-320a on osteogenic differentiation. Conclusion: These results suggest that miR-320a acts as a critical regulator of osteogenic differentiation of hMSCs by repressing its target HOXA10.

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Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells

Cited by 54 publications

References 19 publications

Fibroblast involvement in soft connective tissue calcification

Fibroblast involvement in soft connective tissue calcification

Do Epigenetic Marks Govern Bone Mass and Homeostasis?

MicroRNA-320a Regulates the Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by Targeting HOXA10

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