“…Cell localization and distribution on the biomaterial can be analyzed by simply labeling the nuclei at different timepoints after cell seeding on the scaffold [ 52 ]. The morphology of the biomaterial-seeded cells can also be examined by scanning electron microscopy (SEM) [ 14 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 ], as well as the intracellular structures by transmission electron microscopy (TEM) [ 65 , 68 ]. Cell morphology and distribution can also be assessed using immunofluorescence staining of actin filaments, by means of anti-phalloidin antibodies among others, which allows for the analysis of the cytoskeleton conformation of cell growth in contact with the biomaterials [ 14 , 52 , 54 , 55 , 57 , 60 , 62 , 64 , 65 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 ].…”