Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

Apinun, Jirun; Honsawek, Sittisak; Kuptniratsaikul, Somsak; Jamkratoke, Jutarat; Kanokpanont, Sorada

doi:10.1515/abm-2019-0030

Cited by 9 publications

(11 citation statements)

References 26 publications

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“…Scaffolds based on silk fibroin and silk fibroin with 0.05% and 0.01% addition of collagen with a surfactant as a substance to gelation were studied by Apinun et al [ 36 ]. Collagen combined in the hydrogels yielded a positive effect on matrix formation and proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Collagen combined in the hydrogels yielded a positive effect on matrix formation and proliferation. However, the authors said that there have to be more studies in order to better understand how to optimize the system as a three-dimensional material and cell carrier in bone tissue regeneration [ 36 ]. On the other hand, according to Zeng et al [ 37 ], materials composed form silk fibroin and collagen in 40/60 and in 60/40 weight ratio were good biomaterials for bone tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

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Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

et al. 2021

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Blending of different biopolymers, e.g., collagen, chitosan, silk fibroin and cross-linking modifications of these mixtures can lead to new materials with improved physico-chemical properties, compared to single-component scaffolds. Three-dimensional scaffolds based on three-component mixtures of silk fibroin, collagen and chitosan, chemically cross-linked, were prepared and their physico-chemical and biological properties were evaluated. A mixture of EDC (N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) was used as a cross-linking agent. FTIR was used to observe the position of the peaks characteristic for collagen, chitosan and silk fibroin. The following properties depending on the scaffold structure were studied: swelling behavior, liquid uptake, moisture content, porosity, density, and mechanical parameters. Scanning Electron Microscopy imaging was performed. Additionally, the biological properties of these materials were assessed, by metabolic activity assay. The results showed that the three-component mixtures, cross-linked by EDC/NHS and prepared by lyophilization method, presented porous structures. They were characterized by a high swelling degree. The composition of scaffolds has an influence on mechanical properties. All of the studied materials were cytocompatible with MG-63 osteoblast-like cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

et al. 2021

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“…To promote bone regeneration, Apinun et al [ 109 ] obtained silk fibroin and silk fibroin with 0.05% and 0.01% addition of collagen with a surfactant as a substance to gelation [ 109 ]. Collagen combined in the hydrogels yielded a positive effect on proliferation and matrix formation.…”

Section: Blendingmentioning

confidence: 99%

“…Collagen combined in the hydrogels yielded a positive effect on proliferation and matrix formation. However, authors expected more, and they suggested further studies to better understand and optimize the system as a scaffold and cell carrier in bone tissue regeneration [ 109 ]. On the other hand, materials-based silk fibroin/collagen 40/60 and 60/40 were good materials for bone tissue engineering in the research of Zeng et al [ 110 ].…”

Section: Blendingmentioning

confidence: 99%

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Grabska-Zielińska

Sionkowska

2021

Materials

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This review supplies a report on fresh advances in the field of silk fibroin (SF) biopolymer and its blends with biopolymers as new biomaterials. The review also includes a subsection about silk fibroin mixtures with synthetic polymers. Silk fibroin is commonly used to receive biomaterials. However, the materials based on pure polymer present low mechanical parameters, and high enzymatic degradation rate. These properties can be problematic for tissue engineering applications. An increased interest in two- and three-component mixtures and chemically cross-linked materials has been observed due to their improved physico-chemical properties. These materials can be attractive and desirable for both academic, and, industrial attention because they expose improvements in properties required in the biomedical field. The structure, forms, methods of preparation, and some physico-chemical properties of silk fibroin are discussed in this review. Detailed examples are also given from scientific reports and practical experiments. The most common biopolymers: collagen (Coll), chitosan (CTS), alginate (AL), and hyaluronic acid (HA) are discussed as components of silk fibroin-based mixtures. Examples of binary and ternary mixtures, composites with the addition of magnetic particles, hydroxyapatite or titanium dioxide are also included and given. Additionally, the advantages and disadvantages of chemical, physical, and enzymatic cross-linking were demonstrated.

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“…[ 4 ] Moreover, cell encapsulation has been used in the field of tissue regeneration. [ 6 ] In this case, the matrix acts as a scaffold which allows the encapsulated cells to proliferate and differentiate in the capsules. [ 2 ]…”

Section: Introductionmentioning

confidence: 99%

Biofabrication and Characterization of Alginate Dialdehyde‐Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application

Reakasame

Jin

Zheng

et al. 2020

Macromolecular Bioscience

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cells are used. [2] This technique enables cell transplantation into the host body without using immunosuppressant drugs [1] and simultaneously avoiding cells to disperse from the targeted site. [5] Microand macroencapsulation approaches have been explored in cell-based therapy applications to treat many diseases, especially, diabetes. [4] Moreover, cell encapsulation has been used in the field of tissue regeneration. [6] In this case, the matrix acts as a scaffold which allows the encapsulated cells to proliferate and differentiate in the capsules. [2] Biomaterials that have been widely used for cell encapsulation are hydrogels [7,8] because they can provide a highly hydrated environment which is suitable for cell and tissue growth. [9] Alginatebased hydrogels have been widely used in tissue engineering (TE) because of their biocompatible character. [10] However, alginate hydrogels have some limitations including a slow degradability and poor cell adhesion capability. [11] Degradability of alginate can be improved, for example, by the oxidation of alginate leading to alginate dialdehyde (ADA). [10] Moreover, combination of specific proteins with alginate based hydrogels can improve the cell-matrix interaction of the hydrogel. [12] Gelatin (GEL) is the most popular protein which has been incorporated into ADA based hydrogels for TE applications forming ADA-GEL hydrogels. [10] Because gelatin contains a cellular binding motif, namely arginineglycine-aspartic acid sequence, ADA-GEL can improve the adhesion, differentiation, and proliferation of cells. [13] Gelatin can covalently crosslink with ADA through the Schiff base formation between the amino groups of lysine and hydroxylysine of gelatin and the aldehyde groups of ADA. [14,15] The extrusion method is the most common microencapsulation technique which needs only simple equipment for the fabrication process. With this approach, cell-laden microcapsules are produced by extruding the hydrogel precursor containing cells through a small needle to generate droplets of the mixture into an appropriate hardening bath which will harden the hydrogel precursor. Because ADA based hydrogels can be easily crosslinked using CaCl 2 solution, this approach has been used efficiently for the fabrication of ADA-GEL based microcapsules. However, the diameter of the microcapsules fabricated by this method is strongly related to the properties of the hydrogel precursor. [2] The The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated. The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively. Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0...

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Osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells encapsulated in Thai silk fibroin/collagen hydrogel: a pilot study in vitro

Cited by 9 publications

References 26 publications

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

How to Improve Physico-Chemical Properties of Silk Fibroin Materials for Biomedical Applications?—Blending and Cross-Linking of Silk Fibroin—A Review

Biofabrication and Characterization of Alginate Dialdehyde‐Gelatin Microcapsules Incorporating Bioactive Glass for Cell Delivery Application

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