Osteogenic differentiation of umbilical cord and adipose derived stem cells onto highly porous 45S5 Bioglass^®‐based scaffolds

Abstract

In the context of bone tissue engineering (BTE), combinations of bioactive scaffolds with living cells are investigated to optimally yield functional bone tissue for implantation purposes. Bioactive glasses are a class of highly bioactive, inorganic materials with broad application potential in BTE strategies. The aim of this study was to evaluate bioactive glass (45S5 Bioglass(®)) samples of composition: 45 SiO2, 24.5 CaO, 24.5 Na2O, and 6 P2O5 (in wt%) as scaffold materials for mesenchymal stem cells (MSC). Pore architecture of the scaffolds as well as cell behavior in the three-dimensional environment was evaluated by several methods. Investigations concerned the osteogenic cell attachment, growth and differentiation of adipose tissue derived MSC (adMSC) compared with MSC from human full term umbilical cord tissues (ucMSC) on porous Bioglass(®)-based scaffolds over a cultivation period of 5 weeks. Differences in lineage-specific osteogenic differentiation of adMSC and ucMSC on Bioglass(®) samples were demonstrated. The investigation led to positive results in terms of cell attachment, proliferation, and differentiation of MSC onto Bioglass(®)-based scaffolds confirming the relevance of these matrices for BTE applications.

Bioactive glass containing 90% SiO 2 in hard tissue engineering: An in vitro and in vivo characterization study

“…In accordance with our in vivo data, other studies have already shown successful results of BG grafting (Detsch et al, ; Wu et al, ; Xynos et al, ; Zhang et al, ) and with similar resorbable characteristics (Nommeots‐Nomm et al, ). As far as we know, this is the first time that BGs have been grafted using the tibia defect model comparing six different experimental times, which clearly shows the native physiology of the tibia bone.…”

Superior biofunctionality of dental implant fixtures uniformly coated with durable bioglass films by magnetron sputtering

“…Here, we aimed at obtaining data about cytotoxicity of the RF-MS BG coatings with respect to stem cells (extracted from human dental pulp). Nowadays, numerous researchers are inclined to search for biomaterial surfaces that would induce stem cells differentiation into osteoproductive cells [62][63][64], considering this feature as prerequisite for osseointegration and rapid healing. Although this may seem at a first glance like as a good thing, it may be disastrous on a long term, since all stem cells from the implant vicinity would tend to differentiate quickly to osteoblasts and promote bone formation, in the meantime conducting to an exhaustion of the stem cell pool.…”

Strategies to engineer tendon/ligament-to-bone interface: Biomaterials, cells and growth factors

“…Commonly used biomaterials for bone include calcium phosphate ceramics (in particular hydroxyhapatite (HA) [28][29][30] and β-tricalciumphosphate (β-TCP) [28,[31][32][33]), bioactive glasses (mainly silicate-based 45S5 Bioglass ® [34,35]), natural polymers (such as alginate [36], silk [10,29,37] and collagen [38][39][40][41][42]) and synthetic polymers (particularly poly(ɛ-caprolactone) (PCL) [31,32,[43][44][45], poly-L-lactic acid (PLLA) [33], polyglycolic acid (PGA) [45] and polylactid-co-glycolid acid (PLGA) [46,47]) (table 2).…”