Osteogenic Potential of Decellularized Tooth Matrix

Иванов, А. А.; Latyshev, A. V.; Буторина, Н. Н.; Domoratskaya, E. I.; Данилова, Т. В.; Попова, О. П.

doi:10.1007/s10517-020-04920-8

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…The decellularization of the PDL strips and tooth particles was carried out by sequential incubation with a 1% SDS solution (Sigma-Aldrich, St. Louis, MO, USA), a 1% Triton X-100 solution (Sigma-Aldrich, USA), and a DNase solution (20 µg/mL; Sigma-Aldrich, USA) in 4.2 mM MgCl 2 (Sigma-Aldrich, USA) [35]. Further, the samples were incubated in DMEM culture medium (Gibco, Thermo Fisher Scientific, USA) with antibiotics (300 U/mL penicillin, 300 µg/mL streptomycin, and 75 µg/mL amphotericin B) (Gibco, USA) and stored at −70 • C. All treatment steps were applied to samples at room temperature with constant gentle agitation of the samples in an orbital shaker (Corning™ LSE™, USA).…”

Section: Decellularization Of Pdl Strips and Tooth Particlesmentioning

confidence: 99%

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel

Ivanov,

Kuznetsova,

Popova

et al. 2023

Cells

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Regeneration of periodontal tissues requires an integrated approach to the restoration of the periodontal ligament, cementum, and alveolar bone surrounding the teeth. Current strategies in endogenous regenerative dentistry widely use biomaterials, in particular the decellularized extracellular matrix (dECM), to facilitate the recruitment of populations of resident cells into damaged tissues and stimulate their proliferation and differentiation. The purpose of our study was to evaluate the effect of the exogenous components of the extracellular matrix (hyaluronic acid, laminin, fibronectin) on the differentiation of periodontal ligament stem cells (PDLSCs) cultured with dECM (combinations of decellularized tooth matrices and periodontal ligament) in a 3D collagen I hydrogel. The immunohistochemical expression of various markers in PDLSCs was assessed quantitatively and semi-quantitatively on paraffin sections. The results showed that PDLSCs cultured under these conditions for 14 days exhibited phenotypic characteristics consistent with osteoblast-like and odontoblast-like cells. This potential has been demonstrated by the expression of osteogenic differentiation markers (OC, OPN, ALP) and odontogenic markers (DSPP). This phenomenon corresponds to the in vivo state of the periodontal ligament, in which cells at the interface between bone and cementum tend to differentiate into osteoblasts or cementoblasts. The addition of fibronectin to the dECM most effectively induces the differentiation of PDLSCs into osteoblast-like and odontoblast-like cells under 3D culture conditions. Therefore, this bioengineered construct has a high potential for future use in periodontal tissue regeneration.

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Section: Decellularization Of Pdl Strips and Tooth Particlesmentioning

confidence: 99%

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel

Ivanov,

Kuznetsova,

Popova

et al. 2023

Cells

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“…The bone tissue engineering strategy by decellularizing xenogeneic or allogeneic bone to construct extracellular matrix (ECM)-derived graft material has provided an alternative promising treatment. ECM provides a supportive three-dimensional microenvironment network for cell adhesion, proliferation, and differentiation. , The decellularized bone matrix (DCB) constructed by removing the cellular and immunogenic components in the ECM of bone in vitro by physical, chemical, and enzymatic methods retains the structural characteristics and represents the native bone-specific microenvironment of ECM. , The DCB is similar with natural bone tissue in biochemical components, biophysical characteristics such as pore size, porosity, and mechanics. These physical and chemical properties can not only provide a mechanically stable microenvironment but also regulate the fate of cells by affecting cell adhesion, migration, proliferation, and differentiation .…”

Section: Introductionmentioning

confidence: 99%

“…6,7 The decellularized bone matrix (DCB) constructed by removing the cellular and immunogenic components in the ECM of bone in vitro by physical, chemical, and enzymatic methods retains the structural characteristics and represents the native bonespecific microenvironment of ECM. 8,9 The DCB is similar with natural bone tissue in biochemical components, biophysical characteristics such as pore size, porosity, and mechanics. These physical and chemical properties can not only provide a mechanically stable microenvironment but also regulate the fate of cells by affecting cell adhesion, migration, proliferation, and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Decellularized Bone Matrix Promotes Bone Regeneration by Releasing Osteogenic Peptides

Cui

Wang

Tian

et al. 2023

ACS Biomater. Sci. Eng.

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The decellularized bone matrix (DCB) provides a promising bone substitute for the treatment of bone defects because of its similar biochemical, biophysical, and mechanical properties to normal bone tissue. However, the decellularized procedure also greatly reduced its osteogenic induction activity. In this study, peptides derived from the knuckle epitope of bone morphogenetic protein-2 were incorporated into the thermosensitive hydrogel poloxamer 407, and the peptide-loaded hydrogel was then filled into the pores of DCB to construct a functionalized scaffold with enhanced osteogenesis. In vitro studies have shown that the functionalized DCB scaffold possessed appropriate mechanical properties and biocompatibility and exhibited a sustained release profile of osteogenic peptide. These performances critically facilitated cell proliferation and cell spreading of bone marrow mesenchymal stem cells and upregulated the expression of osteogenic-related genes by activating the Smad/Runx2 signaling pathway, thereby promoting osteogenic differentiation and extracellular matrix mineralization. Further in vivo studies demonstrated that the functionalized DCB scaffold accelerated the repair of critical radial defects in rabbits without inducing excessive graft-related inflammatory responses. These results suggest a clinically meaningful strategy for the treatment of large segmental bone defects, and the prepared osteogenic peptide modified composite DCB scaffold has great application potential for bone regeneration.

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