Osteogenic potential of in vitro osteo-induced adipose-derived mesenchymal stem cells combined with platelet-rich plasma in an ectopic model

Cvetković, Vladimir J.; Najdanović, Jelena G.; Vukelić-Nikolić, Marija; Stojanović, Sanja; Najman, Stevo

doi:10.1007/s00264-015-2929-x

Cited by 30 publications

(24 citation statements)

References 23 publications

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“…The results of these studies, bolstered by the results of in vitro and animal studies, indicate that bone marrow derived MSCs carry little to no risk of malignant transformation, and that they are likely safe for use in human orthopaedic applications [4,7,[13][14][15]. However, no large scale investigations exist with long-term patient follow-up where all complications have been reported, adjudicated, and classified.…”

Section: Introductionmentioning

confidence: 93%

“…Because MSCs can differentiate into bone, cartilage, muscle, tendon, and ligament tissue and can use paracrine and other effects to elicit significant changes in injured tissues, their use for treating orthopaedic conditions holds significant promise [2][3][4][5]. In a clinical setting MSCs are typically harvested from bone marrow, then isolated and either re-injected or implanted in the same surgical procedure or culture expanded and then used clinically.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions

Centeno

Al‐Sayegh

Freeman

et al. 2016

International Orthopaedics (SICOT)

130

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Introduction The purpose of the present investigation is to report on detailed complications among a much larger group of 2372 orthopaedic patients treated with stem cell injections who were followed in a treatment registry for up to nine years. Methods All patients underwent an MSC-based, percutaneous injection treatment of an orthopaedic condition between December 2005 and September 2014 at one of 18 clinical facilities. Treated areas of the body included the knee, hip, ankle/foot, hand/wrist, elbow, shoulder, and spine. The patients were followed prospectively via enrollment in a treatment registry. Patients were followed prospectively at one, three, six and 12 months, and annually thereafter, using an electronic system, ClinCapture software.Results A total of 3012 procedures were performed on 2372 patients with follow-up period of 2.2 years. A total of 325 adverse events were reported. The majority were pain postprocedure (n = 93, 3.9 % of the study population) and pain due to progressive degenerative joint disease (n = 90, 3.8 % of the study population). Seven cases reported neoplasms, a lower rate than in the general population. The lowest rate of adverse events was observed among patients injected with BMC alone. Conclusion Lowest rate of adverse events was among those patients receiving BMC injections alone, but the higher rate of AEs for BMC plus adipose and cultured cells was readily explained by the nature of the therapy or the longer followup. There was no clinical evidence to suggest that treatment Electronic supplementary material The online version of this article

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions

Centeno

Al‐Sayegh

Freeman

et al. 2016

International Orthopaedics (SICOT)

130

View full text Add to dashboard Cite

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“…Therefore, a question regarding interactions between cells of fat origin and the CaP coating has arisen despite the poor (1 of 9 cases) EO features of hAMSCs not treated with recombinant BMP2 and subcutaneously implanted in immunodeficient mice on porous CaP ceramic particles [ 54 ]. At 8 weeks, weak vascularization and EO induced by mouse AMSCs immobilized on bone substitute material Bio-Oss were reported [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Costimulatory Effect of Rough Calcium Phosphate Coating and Blood Mononuclear Cells on Adipose-Derived Mesenchymal Stem Cells In Vitro as a Model of In Vivo Tissue Repair

et al. 2020

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Calcium phosphate (CaP) materials do not always induce ectopic vascularization and bone formation; the reasons remain unclear, and there are active discussions of potential roles for post-implantation hematoma, circulating immune and stem cells, and pericytes, but studies on adipose-derived stem cells (AMSCs) in this context are lacking. The rough (average surface roughness Ra = 2–5 µm) scaffold-like CaP coating deposited on pure titanium plates by the microarc oxidation method was used to investigate its subcutaneous vascularization in CBA/CaLac mice and in vitro effect on cellular and molecular crosstalk between human blood mononuclear cells (hBMNCs) and AMSCs (hAMSCs). Postoperative hematoma development on the CaP surface lasting 1–3 weeks may play a key role in the microvessel elongation and invasion into the CaP relief at the end of the 3rd week of injury and BMNC migration required for enhanced wound healing in mice. Satisfactory osteogenic and chondrogenic differentiation but poor adipogenic differentiation of hAMSCs on the rough CaP surface were detected in vitro by differential cell staining. The fractions of CD73+ (62%), CD90+ (0.24%), and CD105+ (0.41%) BMNCs may be a source of autologous circulating stem/progenitor cells for the subcutis reparation, but allogenic hBMNC participation is mainly related to the effects of CD4+ T cells co-stimulated with CaP coating on the in vitro recruitment of hAMSCs, their secretion of angiogenic and osteomodulatory molecules, and the increase in osteogenic features within the period of in vivo vascularization. Cellular and molecular crosstalk between BMNCs and AMSCs is a model of effective subcutis repair. Rough CaP surface enhanced angio- and osteogenic signaling between cells. We believe that preconditioning and/or co-transplantation of hAMSCs with hBMNCs may broaden their potential in applications related to post-implantation tissue repair and bone bioengineering caused by microarc CaP coating.

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“…Particularly, materials based on hydroxyapatite and β-tricalcium phosphate are suitable for BTE [42]. In addition, materials based on bone mineral matrix are frequently used [43][44][45][46]. Also, regulatory factors are not less important components in BTE, and at the first place, they should induce and support osteogenic differentiation, adhesion, and proliferation of implanted cells.…”

Section: Bone Substitutes and Regulatory Factorsmentioning

confidence: 99%

“…Finally, there is a growing interest about synergistic effects of ADSCs and PRP for bone regeneration. Many studies reported promising osteogenic potential of ADSCs and PRP combination [46,55,56,59] and stimulative potential of PRP that improves osteogenesis in combination with cells [51,60]. In addition, Fernandes and Yang [49] reviewed and summarized some recently published data which implies that adipose-derived stem cells obtained from human, mouse, rat and rabbit in combination with PRP in in vitro and/or in vivo conditions are related with outcomes which are of benefit for BTE [49].…”

Section: Bone Substitutes and Regulatory Factorsmentioning

confidence: 99%

Application of Adipose-Derived Stem Cells in Treatment of Bone Tissue Defects

Najman¹,

Najdanović²,

Cvetković³

2021

Clinical Implementation of Bone Regeneration and Maintenance

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Despite excellent self-regeneration capacity of bone tissue, there are some large bone defects that cannot be healed spontaneously. Numerous literature data in the field of cell-based bone tissue engineering showed that adipose-derived stem cells (ADSCs) after isolation could be subsequently applied in a one-step approach for treatment of bone defect, without previous in vitro expansion and osteoinduction. However, standard approaches usually involve in vitro expansion and osteoinduction of ADSCs as an additional preparation step before its final application. Bioreactors are also used for the preparation of ADSC-based graft prior application. The commonly used approaches are reviewed, and their outcomes, advantages, disadvantages, as well as their potential for successful application in the treatment of bone defects are discussed. Difficulty in spontaneous healing of bone defects is very often due to poor vascularization. To overcome this problem, numerous methods in bone tissue engineering (BTE) were developed. We focused on freshly isolated stromal vascular fraction (SVF) cells and ADSCs in vitro induced into endothelial cells (ECs) as cells with vasculogenic capacity for the further application in bone defect treatment. We have reviewed orthotopic and ectopic models in BTE that include the application of SVFs or ADSCs in vitro induced into ECs, with special reference to co-cultivation.

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Osteogenic potential of in vitro osteo-induced adipose-derived mesenchymal stem cells combined with platelet-rich plasma in an ectopic model

Abstract: The combination of in vitro osteo-induced ADSCs and PRP on BMM carrier represents a promising approach for bone regeneration.

Cited by 30 publications

References 23 publications

A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions

A multi-center analysis of adverse events among two thousand, three hundred and seventy two adult patients undergoing adult autologous stem cell therapy for orthopaedic conditions

Costimulatory Effect of Rough Calcium Phosphate Coating and Blood Mononuclear Cells on Adipose-Derived Mesenchymal Stem Cells In Vitro as a Model of In Vivo Tissue Repair

Application of Adipose-Derived Stem Cells in Treatment of Bone Tissue Defects

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