Osteological development and abnormalities of the vertebral column and caudal skeleton in larval and juvenile stages of hatchery-reared Senegal sole (Solea senegalensis)

Gavaia, Paulo J.; Dinis, María Teresa; Cancela, M. Leonor

doi:10.1016/s0044-8486(02)00167-9

Cited by 138 publications

(203 citation statements)

References 34 publications

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“…Moreover, vertebral deformities may also occur later in ontogeny (i.e. during the ongrowing period), at which point they are generally induced by mechanical overloads (Kranenbarg et al 2005) or by the curvature of the vertebral axis (Gorman et al 2010), or a combination of both (Gavaia et al 2002, Cardeira et al 2012. Irrespective of the cause of vertebral anomalies, they can compromise the biological performance of fish.…”

Section: Discussionmentioning

confidence: 99%

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Ortiz-Delgado¹,

Fernández²,

Sarasquete³

et al. 2014

Aquat. Biol.

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During the last decades, the finfish larviculture industry has considerably improved its rearing methods under intensive conditions, but larval quality remains one of the main problems for the proper sustainable success of this productive sector (Kou moundouros 2010, Boglione et al. 2013a). The quality of farmed fish is directly related to the quality of the fry, and depends on both organoleptic and morphological characteristics that should be as similar as possible to that of wild fish, which is considered to be the ABSTRACT: This study provides a comprehensive description of the tissue organization of nondeformed and deformed opercula and vertebrae from gilthead sea bream Sparus aurata juveniles by means of histological, histochemical and immunohistochemical approaches. Two types of opercular anomalies are described: the folding of the opercle and subopercle into the gill chamber, starting at the upper corner of the branchial cleft and extending down to its lower third; and the partial lack of the operculum (opercle, subopercle, interopercle and preopercle underdeveloped) with a regression of the loose edge extending down to its lower third. Histological observations revealed a rare type of bone remodelling process in the opercular structure, which consisted of the coalescence of contacting bone tissues (presumably from the preopercle and opercle), resulting in skeletal tissue with a trabecular aspect filled by a single-cell epithelium of cubic osteoblastic-like cells. Differences in collagen fiber thickness and its 3-dimensional arrangement between normal and deformed opercula were also found. Lordotic vertebrae were characterized by the formation of fibrous cartilage in the haemal and/or neural sides, indicating that a metaplastic shift occurred during the process of lordosis. Another major histomorphological change found in lordotic vertebrae was the complete loss of notochordal sheath integrity. Histological alterations were coupled with an imbalance of cell death and cell proliferation processes in lordotic vertebrae as well as that of bone formation/resorption, and extracellular matrix deposition activity differences which might have resulted from the remodelling process occurring in lordotic vertebrae. Altogether, these results provide an increase in our basic knowledge of bone disorders that contribute to our understanding of the mechanisms by which these skeletal anomalies appear in this fish species and which hamper its production efficiency.

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Section: Discussionmentioning

confidence: 99%

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Ortiz-Delgado¹,

Fernández²,

Sarasquete³

et al. 2014

Aquat. Biol.

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“…Vertebral deformities were a slight deformation of the centra with a low frequency (7.7%) compared to other hatchery-reared species where larval deformities of 24% to 44% have been reported (10,19,20). Boglione et al (16) reported that only 4% of wild caught S. aurata shows body deformations, in contrast to the very high values observed in hatchery-reared larvae (21).…”

Section: Discussionmentioning

confidence: 89%

“…Besides, they were observed called as scoliosis, lordosis and kyphosis, and also observed Z, V and ˄ type curve of vertebra (6,8). To this date, although larval phase was evaluated until juvenile stage (2,3,9,10), recent studies were focused on skeletogenesis mainly depend on bone and cartilage developmental stages with notochordal ontogeny (11,12). However, it is well recorded that factors effecting skeletal development in larval fish are malnutrition and/or sudden changes in biotical and abiotical factors under culture conditions (8,11,12).…”

Section: Introductionmentioning

confidence: 99%

Skeletal deformities of cultured sharpsnout seabream (Diplodus puntazzo) larvae during early life development

ÇOBAN

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, skeletal deformities were investigated during early life stages in sharpsnout seabream, Diplodus puntazzo, larvae during larval development period. Whole larval period (1-42 days after hatching, DAH) was examined in three stages (preflexion, flexion and postflexion) based on notochord curve. Totally, 1210 specimens were examined during the experiment but 689 deformities were recorded in 256 larvae. Also, deformity rate was calculated as 21.2% at the end of the experiment. In terms of notochord flexion at stage-specific, no differences were found between preflexion (19.7%) and postflexion (15.8%) stages (p>0.05) while there was a significant difference during flexion stage (32.8%) in comparison with other groups (p<0.05). Correspondence analysis (CA) was performed in order to describe of skeletal deformities mainly depend on notochord flexion. According to CA analysis, it was determined that deformities were closely related with both flexion stages quantitatively and also postflexion stages qualitatively.Keywords: D. puntazzo, larval development, skeletal deformities, notochord flexion. Kültür koşullarındaki sivriburun karagöz (Diplodus puntazzo) larvalarında erken dönem iskelet deformasyonlarıÖzet: Bu çalışmada, yetiştiriciliği yapılan sivriburun karagöz (Diplodus puntazzo) larvalarının erken hayat döngüsü içerisinde iskelet sisteminde meydana gelen deformasyonlar incelenmiştir. Deneme boyunca larval dönem (1-42 gün) notokorda da meydana gelen değişimlere göre üç bölüme ayrılmıştır (bükülme öncesi, bükülme sırası ve bükülme sonrası). Deneme boyunca toplamda 1210 larva incelenmiş ve 256 deforme larvada 689 iskelet deformasyonu tespit edilmiştir. Deneme sonunda deformasyon oranı %21,2 hesaplanmıştır. Larval dönemdeki safhalara göre deformasyon oranları bükülme öncesi (%19,7) ile bükülme sonrası (%15,8) önemsiz bulunurken (p>0,05), bükülme sırası (%32,8) evresi önemlilik arz etmiştir (p<0,05). Notokordaya göre iskelet deformasyonları Korrespond Analiz yöntemi kullanılarak yapılmıştır. Bu analiz yöntemine göre iskelet deformasyonları safhalar arasında hem niteliksel olarak farklılıkları ortaya koymuş hem de bükülme sonrası dönemde niceliksel ayrımı belirtmiştir. Anahtar sözcükler: D. puntazzo, larval gelişim, iskelet deformasyonu, notokorda bükülmesi.

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“…The reasons for this include lack of full control over spawning, poor fry quality and high mortality rates during the weaning stage (Cañavate and Fernández-Díaz 1999;Anguís and Cañavate 2005), all leading to juvenile scarcity for stocking purposes. This problem is compounded by a high incidence of skeletal malformations in post-larvae and juveniles (Gavaia et al 2002) and of disease outbursts affecting all ontogenetic stages caused by multiple pathogenic agents (Zorrilla et al 2003). Furthermore, optimization of production has not been possible due to high heterogeneity in growth rates within cultured stocks that result in high body size variance at harvest (Dinis et al 1999, Flos et al 2001.…”

Section: Introductionmentioning

confidence: 99%

Loss of genetic variability in a hatchery strain of Senegalese sole (<i>Solea senegalensis</i>) revealed by sequence data of the mitochondrial DNA control region and microsatellite markers

Sánchez¹,

Viñas²,

Bremer³

et al. 2012

Sci. Mar.

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SUMMARY: Comparisons of the levels of genetic variation within and between a hatchery F 1 (FAR, n=116) of Senegalese sole, Solea senegalensis, and its wild donor population (ATL, n=26), both native to the SW Atlantic coast of the Iberian peninsula, as well as between the wild donor population and a wild western Mediterranean sample (MED, n=18), were carried out by characterizing 412 base pairs of the nucleotide sequence of the mitochondrial DNA control region I, and six polymorphic microsatellite loci. FAR showed a substantial loss of genetic variability (haplotypic diversity, h=0.49±0.066; nucleotide diversity, p=0.006±0.004; private allelic richness, pAg=0.28) to its donor population ATL (h=0.69±0.114; p=0.009±0.006; pAg=1.21). Pairwise F ST values of microsatellite data were highly significant (P<0.0001) between FAR and ATL (0.053) and FAR and MED (0.055). The comparison of wild samples revealed higher values of genetic variability in MED than in ATL, but only with mtDNA CR-I sequence data (h=0.948±0.033; p=0.030±0.016). However, pairwise F ST and F ST values between ATL and MED were highly significant (P<0.0001) with mtDNA CR-I (0.228) and with microsatellite data (0.095), respectively. While loss of genetic variability in FAR could be associated with the sampling error when the broodstock was established, the results of parental and sibship inference suggest that most of these losses can be attributed to a high variance in reproductive success among members of the broodstock, particularly among females.Keywords: Solea senegalensis, genetic variability, mitochondrial DNA Control Region, microsatellite loci, broodstock management, variance in reproductive success, flatfish. RESUMEN: Pérdida de variabilidad genética en una población de cultivo de lenguado senegalés (Solea SenegalenSiS) revelada mediante datos de secuencia de la región control del ADN mitocondrial y de marcadores microsatélite. -Se compararon los niveles de variabilidad genética de la F 1 de una población de cultivo (FAR, n=116) de lenguado senegalés, Solea senegalensis, y de una muestra de su población de origen (ATL, n=26), ambas provenientes del SO de la península ibérica (Atlántico), así como entre esta última y una muestra de individuos salvajes del Mediterráneo occidental (MED, n=18), mediante la caracterización de una secuencia de 412 pares de bases de la Región Control-I del ADN mitocondrial, y de seis loci microsatélite. FAR experimentó una reducción sustancial de variabilidad genética (diversidad haplotípica, h=0.49±0.066; diversidad nucleotídica, p=0.006±0.004; riqueza de alelos privados, pAg=0.28) respecto a su población original ATL (h=0.69±0.114; p=0.009±0.006; pAg=1.21). Los valores de F ST entre poblaciones, calculados a partir del análisis de los microsatélites, fueron altamente significativos (p<0.0001) para FAR y ATL (0.053), y para FAR y MED (0.055). La comparación de las dos muestras salvajes mostró mayores niveles de variabilidad genética en MED que en ATL, pero únicamente en marcadores mitocondriales (h=0.948±0.033; p=0....

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Osteological development and abnormalities of the vertebral column and caudal skeleton in larval and juvenile stages of hatchery-reared Senegal sole (Solea senegalensis)

Cited by 138 publications

References 34 publications

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Skeletal deformities of cultured sharpsnout seabream (Diplodus puntazzo) larvae during early life development

Loss of genetic variability in a hatchery strain of Senegalese sole (<i>Solea senegalensis</i>) revealed by sequence data of the mitochondrial DNA control region and microsatellite markers

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