Osteomyelitis, Oxidative Stress and Related Biomarkers

Massaccesi, Luca; Galliera, Emanuela; Pellegrini, Antonio; Banfi, Giuseppe; Romanelli, Massimiliano Marco Corsi

doi:10.3390/antiox11061061

Cited by 12 publications

(6 citation statements)

References 120 publications

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“…Mitochondrial dysfunction and apoptosis are also involved in BCO in broilers ( Ferver et al, 2021 ). Although there is no direct evidence linking oxidative stress and BCO pathogenesis, higher levels of oxidative stress in response to local infection have been reported in human patients with chronic osteomyelitis ( Massaccesi et al, 2022 ). The pathology of osteomyelitis is characterized by localized inflammation, bone mineral loss, and structural damage, which share similarities with broiler BCO ( Grbic et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Tompkins¹,

Liu²,

Kim³

2023

Front. Physiol.

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The effects of hydrogen peroxide (H2O2) on the osteogenic differentiation of primary chicken mesenchymal stem cells (MSCs) were investigated. MSCs were subjected to an osteogenic program and exposed to various concentrations of H2O2 for 14 days. Results showed that high concentrations of H2O2 (200 and 400 nM) significantly increased pro-apoptotic marker CASP8 expression and impaired osteogenic differentiation, as indicated by decreased mRNA expression levels of osteogenesis-related genes and reduced in vitro mineralization. In contrast, long-term H2O2 exposure promoted basal expression of adipogenic markers at the expense of osteogenesis in MSCs during osteogenic differentiation, and increased intracellular reactive oxygen species (ROS) production, as well as altered antioxidant enzyme gene expression. These findings suggest that long-term H2O2-induced ROS production impairs osteogenic differentiation in chicken MSCs under an osteogenic program.

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Section: Discussionmentioning

confidence: 99%

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Tompkins¹,

Liu²,

Kim³

2023

Front. Physiol.

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“…Osteomyelitis exhibits elevated levels of oxidative stress biomarkers. [15][16][17] Therefore, the levels of antioxidant enzymes namely CAT, SOD, GSH, and GPx were studied. Genistein increased the antioxidant enzymes by almost 40%-50%.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and anti‐inflammatory effects of genistein in Staphylococcus aureus induced osteomyelitis in rats

Guo

2023

J Biochem & Molecular Tox

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Methicillin-resistant Staphylococcus aureus (MRSA) is a highly infectious Grampositive pathogen known to cause severe diseases such as endocarditis, food poisoning, pneumonia, osteomyelitis, and septicemia. MRSA is a major public health issue. Among these, osteomyelitis is inflammation of the bone caused by the invasion of the bacterial pathogen in the bones. Its prominent symptoms include fever, pain, and redness of bones. In the case of children, it affects the long bones of arms and legs, whereas in the case of adults it affects the hip, feet, and spine.Bacterial osteomyelitis can trigger pathological remodeling of bones and hence causes substantial morbidity and mortality. The present study aims to evaluate the isoflavone genistein's (5,7-dihydroxy-3-(4-hydroxyphenyl)−4H-1-benzopyran-4one,4′,5,7 trihydroxyisoflavone) antimicrobial and anti-inflammatory effects against osteomyelitis induced by MRSA in male Wistar rats. Classification of the animals was into the following: sham (Group I), osteomyelitis (Group II, control), genistein (25 mg/ kg body weight, Group III), and genistein (50 mg/kg body weight, Group IV). The rats did not receive any treatment for 4 weeks after bacterial inoculation. Genistein was then administered twice daily for 2 weeks. Bacterial growth, mean body weight bone infection status, and side effects of genistein treatment were assessed. Furthermore, lipid peroxidation, superoxide dismutase, glutathione (GSH) peroxidase, catalase, reduced GSH, tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 were also determined. Two days after treatment, it was found that genistein significantly suppressed bacterial growth and reduced serum pro-inflammatory cytokines TNF-α and IL-6. Therefore, the study suggests that genistein could be a promising lead against MRSA-induced osteomyelitis.

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“…Besides, excess ROS has ability to activate NFκB and p38 MAPK signaling pathways to promoting M1 macrophage activation and up-regulate the expression of pro-inflammatory chemokines/cytokines, thereby aggravating the inflammatory response [25,26]. These activities could induce osteoclastogenesis; oppositely, the progression of osteoblastogenesis is inhibited [27,28]. To date, a variety of strategies targeting ROS have been proposed to modulate the pathological microenvironment for achieving effectively tissue repair.…”

Section: Introductionmentioning

confidence: 99%

A multifunctional composite hydrogel that sequentially modulates the process of bone healing and guides the repair of bone defects

Lu,

Wang,

Zou

et al. 2024

Biomed. Mater.

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Calcium carbonate (CaCO3), which exhibits excellent biocompatibility and bioactivity, is a well-established bone filling material for bone defects. Here, we synthesized CaCO3 microspheres (CMs) to use as an intelligent carrier to load bone morphogenetic protein-2 (BMP-2). Subsequently, drug-loaded CMs and catalase (CAT) were added to methacrylated gelatin (GelMA) hydrogels to prepare a composite hydrogel for differential release of the drugs. CAT inside hydrogels was released with a fast rate to eliminate H2O2 and generate oxygen. Constant BMP-2 release from CMs induced rapid osteogenesis. Results in vitro indicated that the composite hydrogels efficiently reduced the level of intracellular reactive oxygen species (ROS), preventing cells from being injured by oxidative stress, promoting cell survival and proliferation, and enhancing osteogenesis. Furthermore, animal experiments demonstrated that the composite hydrogels were able to inhibit the inflammatory response, regulate macrophage polarization, and facilitate the healing of bone defects. These findings indicate that a multi-pronged strategy is greatly expected to promote the bone healing by modulating pathological microenvironments.

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Osteomyelitis, Oxidative Stress and Related Biomarkers

Cited by 12 publications

References 120 publications

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Impact of exogenous hydrogen peroxide on osteogenic differentiation of broiler chicken compact bones derived mesenchymal stem cells

Antibacterial and anti‐inflammatory effects of genistein in Staphylococcus aureus induced osteomyelitis in rats

A multifunctional composite hydrogel that sequentially modulates the process of bone healing and guides the repair of bone defects

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