Osteonecrosis in stroke-prone spontaneously hypertensive rats: effect of glucocorticoid

Murata, Masakazu; Kumagai, Kenji; Miyata, Noriaki; Osaki, Makoto; Shindo, Hiroyuki

doi:10.1007/s00776-007-1129-y

Cited by 52 publications

(32 citation statements)

References 15 publications

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“…Nowadays, there are several alternative mechanisms responsible for glucocorticoid (GC)-associated osteonecrosis of femoral head (ONFH) such as fat embolisation [1], intramedullary pressure changes [2], modified artery constriction [3,4], circulatory impairment [5], coagulation disorders [6], and cell apoptosis and dysfunction [7][8][9]. Among them, bone marrow mesenchymal stem cell (MSC) adipogenesis and apoptosis is a principal mechanism involved in the onset and progression of this disease.…”

Section: Introductionmentioning

confidence: 99%

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

et al. 2014

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Section: Introductionmentioning

confidence: 99%

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

et al. 2014

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“…But also the direct evidence that chronic use of methylprednisolone may increase ROS damage15. In addition, it has been hypothesized that osteonecrosis is produced by the ischemic change accompanying the compartment pressure load in the marrow, where degeneration and necrosis may occur simultaneously with oxidative stress16. Besides, antioxidants such as Coenzyme Q10 (CoQ10)17 and hydrogen-rich saline18, have already been proved to be therapeutic for steroid-induced osteonecrosis in rats.…”

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confidence: 99%

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species

Deng

Niu

Zhou

et al. 2017

Sci Rep

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Reducing oxidative stress (ROS) have been demonstrated effective for steroid-induced osteonecrosis of the femoral head (steroid-induced ONFH). Selenium (Se) plays an important role in suppressing oxidative stress and has huge potential in ONFH treatments. However the Se has a narrow margin between beneficial and toxic effects which make it hard for therapy use in vivo. In order to make the deficiency up, a control release of Se (Se@SiO2) were realized by nanotechnology modification. Porous Se@SiO2 nanocomposites have favorable biocompatibility and can reduced the ROS damage effectively. In vitro, the cck-8 analysis, terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) stain and flow cytometry analysis showed rare negative influence by porous Se@SiO2 nanocomposites but significantly protective effect against H2O2 by reducing ROS level (detected by DCFH-DA). In vivo, the biosafety of porous Se@SiO2 nanocomposites were confirmed by the serum biochemistry, the ROS level in serum were significantly reduced and the curative effect were confirmed by Micro CT scan, serum Elisa assay (inflammatory factors), Western blotting (quantitative measurement of ONFH) and HE staining. It is expected that the porous Se@SiO2 nanocomposites may prevent steroid-induced ONFH by reducing oxidative stress.

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“…There are many mechanisms of GC-related ONFH such as disruption of the balance between osteogenic and adipogenic differentiation [7], fat embolization [8], circulatory impairment [9], cell apoptosis and dysfunction [10], intramedullary pressure changes [11], coagulation disorders [12], and modified artery constriction [13]. Among them, disruption of the balance between osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is a principal mechanism involved in the onset and progression of this disease.…”

Section: Introductionmentioning

confidence: 99%

Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats

Huang

Cheng

Cao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Glucocorticoid (GC)-related osteonecrosis of the femoral head (ONFH) is a common complication following administration of steroids to treat many diseases. Our previous study demonstrated that icariin (ICA) might have a beneficial effect on the bone marrow mesenchymal stem cells (BMSCs) of patients with steroid-associated osteonecrosis. In this study, we investigated the underlying mechanisms of ICA associated with the potential enhancement of osteogenesis and anti-adipogenesis in GC-related ONFH. Methods: In vitro cell proliferation was evaluated by CCK-8 assay. Alizarin red S and alkaline phosphatase (ALP) activity were used to measure osteogenic differentiation, while adipogenic differentiation was revealed by oil red O staining and TG content assay. The expression level of osteogenesis-associated genes and PPARγ was evaluated by RT-qPCR, western blotting and immunofluorescence. A total of 30 female SD rats were randomly separated into three groups: a control group, a methylprednisolone (MPS) group and a MPS + ICA group. Serum ALP and TG (triglyceride), micro-CT scanning, histological and immunohistochemical analyses were performed in the animal model. Results: In the in vitro study, ICA promoted proliferation, improved osteogenic differentiation and suppressed adipogenic differentiation of BMSCs treated with MPS. The group treated with MPS and 10^-6 M ICA expressed higher levels of Runx2, ALP, bone morphogenetic protein (BMP) 2, and OC and lower expression of PPARγ than the MPS group. In the in vivo study, ICA prevented bone loss in a rat model of GC-related ONFH as shown by micro-CT scanning, histological and immunohistochemical analyses. Conclusions: ICA is an effective compound for promoting bone repair and preventing or delaying the progression of GC-associated ONFH in rats. This effect can be explained by its ability to improve the balance between adipogenesis and osteogenesis, indicating that ICA is an effective candidate for management of GC-associated ONFH.

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Osteonecrosis in stroke-prone spontaneously hypertensive rats: effect of glucocorticoid

Cited by 52 publications

References 15 publications

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

Treatment of steroid-induced osteonecrosis of the femoral head using porous Se@SiO2 nanocomposites to suppress reactive oxygen species

Icariin Protects against Glucocorticoid-Induced Osteonecrosis of the Femoral Head in Rats

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