Osteoprogenitor response to defined topographies with nanoscale depths

Dalby, Matthew J.; McCloy, David; Robertson, Mark; Wilkinson, C. D. W.; Oreffo, Richard O.C.

doi:10.1016/j.biomaterials.2005.08.028

Cited by 303 publications

(225 citation statements)

References 45 publications

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“…[37][38][39] Researchers have also demonstrated the ability of nanometric biomaterial surfaces to successfully modulate the adhesion and differentiation of osteoprogenitor cells down the osteoblastic pathway. 40 Finally, the use of nanoscale implants has been shown to reduce local fibroblast activity, which may prevent incorporation into host bone and implant failure. 41 The nanofiber scaffold component of TrioMatrix 1 is created from individual strands of porcine skin collagen, also referred to as ''E-Matrix'' in a prior study.…”

Section: Discussionmentioning

confidence: 99%

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Hsu¹,

Polavarapu²,

Riaz³

et al. 2011

Journal Orthopaedic Research

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The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) in spine fusion has led to concerns regarding a potential accompanying inflammatory response. This study evaluates a combination therapy (TrioMatrix 1 ; Pioneer Surgical, Inc., Marquette, MI) comprised of a demineralized bone matrix (DBM), hydroxyapatite, and a nanofiber-based collagen scaffold in a rodent spine fusion model. Thirty-six athymic rats that underwent a posterolateral intertransverse spinal fusion were randomly assigned to 1 of 5 treatment groups: absorbable collagen sponge alone (ACS, negative control), 10 mg rhBMP-2 on ACS (positive control), TrioMatrix 1 , Grafton 1 (Osteotech, Inc., Eatontown, NJ), and DBX 1 (Synthes, Inc., West Chester, PA). Both TrioMatrix 1 and rhBMP-2-treated animals demonstrated 100% fusion rates as graded by manual palpation scores 8 weeks after implantation. This rate was significantly greater than those of the ACS, Grafton 1 , and DBX 1 groups. Notably, the use of TrioMatrix 1 as evaluated by microCT quantification led to a greater fusion mass volume when compared to all other groups, including the rhBMP-2 group. T2-weighted axial MRI images of the fusion bed demonstrated a significant host response associated with a large fluid collection with the use of rhBMP-2; this response was significantly reduced with the use of TrioMatrix 1 . Our results therefore demonstrate that a nanocomposite therapy represents a promising, cost-effective bone graft substitute that could be useful in spine fusions where BMP-2 is contraindicated.

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Section: Discussionmentioning

confidence: 99%

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Hsu¹,

Polavarapu²,

Riaz³

et al. 2011

Journal Orthopaedic Research

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“…7A) have an increased amount of stress fibers and a greater number of focal adhesions compared to a planar surface (control) and exhibit increased osteoblastic functionality. 147 Nanopits with different randomness (Fig. 7B) change the ability of human MSCs to form mature adhesion spots and stimulate the cells to produce bone mineral components in vitro in the absence of osteogenic supplements.…”

Section: Stem Cell Differentiationmentioning

confidence: 99%

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Miyoshi

Adachi

2014

Tissue Engineering Part B: Reviews

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“…Most studies have examined the osteogenic potential of implant surfaces in vitro by using immature osteoblast or osteoblast cell lines (Dalby et al, 2006a;Dalby et al, 2006b;Le Guéhennec et al, 2008a;Le Guéhennec et al, 2008b). However, the fi rst cells to colonise the surface after implantation, are mesenchymal stem cells (MSCs).…”

Section: Introductionmentioning

confidence: 99%

“…In vitro experiments have shown that cells can respond to their micro-environment (Curtis and Varde, 1964;Engler et al, 2006). Other studies have demonstrated that nanotopography infl uenced cell behaviour (Curtis et al, 2001;Stevens and George, 2005;Dalby et al, 2007a;Dalby et al, 2007b). Surface topography induced mechanical stress in the cytoskeleton 85 www.ecmjournal.org S Lavenus et al Mesenchymal stem cells on titanium nanopores that controls gene expression and thus differentiation (Watson, 1991;Kilian et al, 2010;Olivares-Navarrete et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Adhesion and osteogenic differentiation of human mesenchymal stem cells on titanium nanopores

Lavenus

Berreur²,

Trichet³

et al. 2011

eCM

120

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Titanium implants are widely used in orthopaedic and dental surgery. Surface properties play a major role in cell and tissue interactions. The adhesion and differentiation of mesenchymal stem cells were studied as a function of nanostructures. Titanium surfaces with nanopores 30, 150 and 300 nm in diameter were prepared by physical vapour deposition. PCR arrays indicated that the expression of integrins was modulated by the nanopore size. Human Mesenchymal Stem Cells (hMSCs) exhibited more branched cell morphology on Ti30 than on other surfaces. Ti30 and Ti150 induced osteoblastic differentiation while Ti300 had a limited effect. Overall, nanopores of 30 nm may promote early osteoblastic differentiation and, consequently, rapid osseointegration of titanium implants. Keywords

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Osteoprogenitor response to defined topographies with nanoscale depths

Cited by 303 publications

References 45 publications

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

Adhesion and osteogenic differentiation of human mesenchymal stem cells on titanium nanopores

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