Osteostimulatory effect of bone grafts on fibroblast cultures

Abstract

Objective:We analyzed the morphological changes and alkaline phosphatase (ALP) level in fibroblast, which is indicative of their functional ability when cultured in three different commercially available graft materials with osseoconductive property.Materials and Methods:Fibroblasts obtained from fifth passage were seeded within three different bone substitutes (bovine hydroxyapatite [HA] [Osseo-graft®], β-tricalciumphosphate [RTR®], bovine HA [Bio-oss®]) and incubated under standard cell culture conditions. 10 samples in each group were evaluated for cell morphology and alkaline phosphates activity using scanning electron microscopy and spectrophotometric analysis on the 7th day of culture.Results:Fibroblast cultured with RTR® showed changes in morphology and increase in ALP activity when compared to fibroblast cultured with Osseo-graft® and Bio-oss®.Conclusion:Alkaline phosphatase activity was observed in fibroblasts when cultured with three types of commercially available bone grafts. ALP activity was highest when cultured with β-tricalcium phosphate graft material indicating its better bone regenerating capacity of this graft material.

Functional performance evaluation of an alginate/nano-cockle shell powder nanobiocomposite bone scaffold with BMP-2

“…The presence of BMP-2 in the scaffolds could have a chemotactic effect which leads to the increase in cellular infiltration. The ALP expression by fibroblasts was previously noted in a study by Fathima and Harish [18] in which the increase in ALP expression from fibroblasts grown on the tricalcium phosphate bone graft material indicated a bone stimulatory effect. Similar stimulatory effects can be observed in this study in which the expression of ALP was found to be decreasing as mineralization progressed by week 4.…”

“…In this study, we were unable to identify the presence of matured bone tissue or osteoblasts that are known factors for ALP expression. However, ALP enzymes that are known markers for developing bone cells are also expressed by fibroblasts [18,19]. Fibroblasts were the predominant cells that were observed through histological staining from both groups of scaffolds in this study.…”

Histological Bone-Healing Evaluation of Critical-Size Defects Filled with β-Tricalcium Phosphate in Rat Tibiae

“…To better evaluate the development and formation of calcifications in hard tissue, researchers can also use an alkaline phosphatase (ALP) marker, as its enzymatic activity is related to bone cell development [35,36]. A study by Fathima & Harish [36], which compared morphological changes and ALP levels in fibroblasts cultured with various commercially available graft materials, revealed that RTR ® induced significant morphological alterations and elevated ALP activity, signifying its superior bone-regeneration capacity.…”