OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice

Li, Zhiming; Zhang, Yan; Zhang, Xinzong; Cao, Congcong; Luo, Xiaomin; Gui, Yaoting; Tang, Yunge; Yuan, Shuiqiao

doi:10.1186/s12958-021-00779-0

Cited by 4 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serbp1 KO mice were generated and produced by zygote pronuclear microinjection using CRISPR/Cas9 genome editing as our previously described method [ 34 ]. Briefly, 2 single guide RNAs (sgRNAs) (target exon 1 and 3) and Cas9 mRNAs were mixed and injected into C57BL/6 zygotes for generating founder mice.…”

Section: Methodsmentioning

confidence: 99%

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

Wang¹,

Wang²,

Gan³

et al. 2023

Research

Self Cite

View full text Add to dashboard Cite

Stress granules (SGs) are membraneless cytoplasmic condensates that dynamically assemble in response to various stressors and reversibly disassemble after stimulus removal; however, the mechanisms underlying SG dynamics and their physiological roles in germ cell development are elusive. Here, we show that SERBP1 (SERPINE1 mRNA binding protein 1) is a universal SG component and conserved regulator of SG clearance in somatic and male germ cells. SERBP1 interacts with the SG core component G3BP1 and 26S proteasome proteins PSMD10 and PSMA3 and recruits them to SGs. In the absence of SERBP1, reduced 20S proteasome activity, mislocalized valosin containing protein (VCP) and Fas associated factor family member 2 (FAF2), and diminished K63-linked polyubiquitination of G3BP1 during the SG recovery period were observed. Interestingly, the depletion of SERBP1 in testicular cells in vivo causes increased germ cell apoptosis upon scrotal heat stress. Accordingly, we propose that a SERBP1-mediated mechanism regulates 26S proteasome activity and G3BP1 ubiquitination to facilitate SG clearance in both somatic and germ cell lines.

show abstract

Section: Methodsmentioning

confidence: 99%

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

Wang¹,

Wang²,

Gan³

et al. 2023

Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Adad2 and Rnf17 mutant mice were generated produced by zygote pronuclear microinjection using CRISPR/Cas9 genome editing as previously described ( Li et al, 2021 ). Two single guide RNAs targeting exon 1 and Cas9 mRNAs were mixed and injected into C57BL/6 zygotes, followed by implanted into pseudo-pregnant C57BL/6 females.…”

Section: Methodsmentioning

confidence: 99%

ADAD2 interacts with RNF17 in P-bodies to repress the Ping-pong cycle in pachytene piRNA biogenesis

Xiong

Yin

Gui

et al. 2023

Journal of Cell Biology

Self Cite

View full text Add to dashboard Cite

Pachytene piRNA biogenesis is a hallmark of the germline, distinct from another wave of pre-pachytene piRNA biogenesis with regard to the lack of a secondary amplification process known as the Ping-pong cycle. However, the underlying molecular mechanism and the venue for the suppression of the Ping-pong cycle remain elusive. Here, we showed that a testis-specific protein, ADAD2, interacts with a TDRD family member protein RNF17 and is associated with P-bodies. Importantly, ADAD2 directs RNF17 to repress Ping-pong activity in pachytene piRNA biogenesis. The P-body localization of RNF17 requires the intrinsically disordered domain of ADAD2. Deletion of Adad2 or Rnf17 causes the mislocalization of each other and subsequent Ping-pong activity derepression, secondary piRNAs overproduced, and disruption of P-body integrity at the meiotic stage, thereby leading to spermatogenesis arrested at the round spermatid stage. Collectively, by identifying the ADAD2-dependent mechanism, our study reveals a novel function of P-bodies in suppressing Ping-pong activity in pachytene piRNA biogenesis.

show abstract

“…https://doi.org/10.1186/s12958-021-00779-0 Following publication of the original article [1], the authors reported an error in Fig. 1.…”

Section: Correction To: Reprod Biol Endocrinol 19 95 (2021)mentioning

confidence: 99%

OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice

Cited by 4 publications

References 22 publications

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage

ADAD2 interacts with RNF17 in P-bodies to repress the Ping-pong cycle in pachytene piRNA biogenesis

Correction to: OTOGL, a gelforming mucin protein, is nonessential for male germ cell development and spermatogenesis in mice

Contact Info

Product

Resources

About