Otolog Trombositten Zengin Plazma Üreme Mevsimi Dışında Koç Spermatozoalarının Kriyoprezervasyonu Üzerinde Olumlu Etkiye Sahiptir

ALCAY, Selim; Aktar, Ahmet; KOCA, Davut; KILIC, Mehmet Ali; AKKASOGLU, Mustafa; YILMAZ, Mehmet Melih; SAGIRKAYA, Hakan

doi:10.9775/kvfd.2021.26763

Cited by 3 publications

(2 citation statements)

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“…As in a previous study that was conducted by Alcay et al. ( 2022 ), equilibrated semen was placed into 0.25 mL straws, and they were cooled in liquid nitrogen vapour at 3°C/min from 5 to −8°C and at 25°C/min cooling rate using Nicool Plus PC (Air Liquide, Marne‐la‐Vallée Cedex 3). After that, they were cooled from −8 to −120°C.…”

Section: Methodsmentioning

confidence: 99%

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The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

Aktar,

Toker,

Koca

et al. 2024

Veterinary Medicine & Sci

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ObjectivesThis study aimed to examine the effects of supplementation of vitamin D to the egg‐yolk extender on characteristics of frozen–thawed ram semen.MethodsSemen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D: 0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/mL (VITD 100), and then they were frozen. Sperm motility parameters, plasma membrane functional integrity, acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential of the groups were evaluated after sperm thawing.ResultsTotal motility and progressive motility were higher in VITD 50 than in all other groups (p < 0.05). Higher sperm straightness, linearity, and wooble were higher in VITD 50 than in the control group (p < 0.05). A similar pattern of VITD 50 was observed for plasma membrane integrity and mitochondrial membrane potential (p > 0.05).ConclusionsIn the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/mL vitamin D in the extender provided more effective protection than the other doses.

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Section: Methodsmentioning

confidence: 99%

“…Acrosomal integrities of sperm cells were evaluated by the FITC‐conjugated Pisum sativum agglutinin (FITC‐PSA) method (Alcay et al., 2022 ); sperm sample of 10 µL was added to 100 µL of PBS and centrifuged. The supernatant was discarded, and then 100 µL of PBS was added and centrifuged again.…”

Section: Methodsmentioning

confidence: 99%

The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

Aktar,

Toker,

Koca

et al. 2024

Veterinary Medicine & Sci

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The effect of supplementation of vitamin D to the egg-yolk extender on cryopreservation of ram semen

aktar,

Toker,

Koca

et al. 2023

Preprint

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This study aimed to examine the effects of supplementation of vitamin D to the egg-yolk extender on characteristics of frozen-thawed ram semen. Semen samples obtained from adult rams were pooled and divided into five equal volumes. It was reconstituted with extenders containing different concentrations of vitamin D (0 (control), 12.5 (VITD 12.5), 25 (VITD 25), 50 (VITD 50), and 100 ng/ml (VITD 100)) and then they were frozen. Sperm motility, plasmamembrane functionalintegrity, acrosomal integrity, DNA fragmentation and mitochondrial membrane potential of the groups were determined. Among motility values after thawing, the VITD50 group was found to be superior to the other groups (P < 0.05). When the plasma membrane integrity was evaluated, VITD25 and VITD50 groups were found better than the other groups (P < 0.05). Acrosomal integrity, DNA fragmentation, and mitochondrial membrane potential rates were found better in VITD25, VITD50 and VITD100 groups when compared to all other groups, but no significant difference was found among all groups (P > 0.05). In the study, it was observed that adding vitamin D to the extender had a beneficial effect on ram spermatological parameters. In addition, it was concluded that the use of the 50 ng/ml vitamin D in the extender provided more effective protection than the other doses.

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Role of Platelet-Rich Plasma in the Management of Non-obstructive Azoospermia

Alharbi

2024

Cureus

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Non-obstructive azoospermia (NOA) is a common cause of infertility in males, which is characterized by the absence of sperm in the ejaculate, resulting from impaired spermatogenesis. The primary therapeutic approaches for the management of NOA include testicular sperm extraction, varicocelectomy in case of clinical varicoceles, and hormonal manipulation. While traditional treatments are found to have a limited role in the management of NOA, recent studies have explored the potential of platelet-rich plasma (PRP) as a safer and promising therapeutic option. Autologous PRP preparations are derived from the patient's blood and comprise growth factors and cytokines, promoting tissue repair and regeneration. PRP has a wide range of applications in the medical field, including managing infertility in males and females. This literature review aimed to evaluate the existing evidence on the efficacy and safety of PRP in the management of NOA. After a thorough review of relevant data from observational and experimental studies, the findings of this study suggested that PRP may positively influence spermatogenesis, sperm quality, DNA integrity, and sperm retrieval during assisted reproductive procedures. Further research is needed to establish the optimal PRP preparation, administration method, and long-term benefits. The newer studies shall include a diverse patient population and employ long-term follow-up to assess the durability of any positive effects of PRP treatment. The growing body of evidence regarding the therapeutic potential for NOA in humans offers greater opportunities for men seeking fertility treatment, informing clinical practice, and optimizing the use of PRP.

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Otolog Trombositten Zengin Plazma Üreme Mevsimi Dışında Koç Spermatozoalarının Kriyoprezervasyonu Üzerinde Olumlu Etkiye Sahiptir

Cited by 3 publications

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The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

The effects of supplementation of vitamin D to the egg‐yolk extender on cryopreservation of ram semen

The effect of supplementation of vitamin D to the egg-yolk extender on cryopreservation of ram semen

Role of Platelet-Rich Plasma in the Management of Non-obstructive Azoospermia

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