<p class="abstract"><strong>Background:</strong> The term otomycosis is used to describe fungal infection of ear i.e. external auditory canal, eardrum. This condition appears as a primary infection or develops along with external bacterial infections as a results of antibiotic therapy. This study was designed to determine the clinical symptoms and fungal flora of otomycosis cases.</p><p class="abstract"><strong>Methods:</strong> A total 100 (53% males, 47% females) clinically diagnosed otomycosis cases between 06-70 years were included. The external auditory meatus debris was collected by sterile swabs and sent to the microbial laboratory investigations. </p><p class="abstract"><strong>Results:</strong> Highest prevalence was seen in the age group 21-30 years. Itching in the ear was commonest symptom in 89%, followed by ear ache. Positive fungal growth was seen in 94% cases. <em>Aspergillus</em> was the common isolate in 63% cases, followed by <em>Aspergillus niger</em> with <em>Candida albicans</em> (10%), <em>penicillium</em> in 4% cases and <em>Candida</em> in 3% cases. Coagulase negative <em>Staphylococcus </em>was seen in 32% cases, followed by <em>Pseudomonas aeruginosa</em> (12%), <em>Staphylococcus aureus</em> (16%), <em>E. coli</em> (11%).</p><p><strong>Conclusions:</strong> The genus most commonly involved in otomycosis is <em>Aspergillus</em> followed by <em>Candida</em><strong>. </strong>Clinical improvement was seen in 91% of cases and most of the cases responded satisfactorily with topical clotrimazole. </p>