Ototoxicity caused by topical administration of gentamicin versus tobramycin in rabbits

Oğhan, Fatih; Apuhan, Tayfun; Yılmaz, Fatma

doi:10.1016/j.ijporl.2011.04.005

Cited by 11 publications

(10 citation statements)

References 14 publications

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“…However, in developing countries, aminoglycosides are more likely to result in ototoxic damage secondary to widespread use without physician monitoring and, infrequent audiologic evaluation (Oghan et al 2011). Social factors such as stress and poor nutritional status may also predispose patients to aminoglycoside ototoxicity and increase drug-induced hearing damage particularly in developing nations (Forge and Schacht 2000).…”

Section: Discussionmentioning

confidence: 99%

d -Methionine reduces tobramycin-induced ototoxicity without antimicrobial interference in animal models

Fox

Cooper

Speil

et al. 2016

Journal of Cystic Fibrosis

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Background Tobramycin is a critical cystic fibrosis treatment however it causes ototoxicity. This study tested D-methionine protection from tobramycin-induced ototoxicity and potential antimicrobial interference. Methods Auditory brainstem responses (ABR) and outer hair cell (OHC) quantifications measured protection in guinea pigs treated with tobramycin and a range of D-methionine doses. In vitro antimicrobial interference studies tested inhibition and post antibiotic effect assays. In vivo antimicrobial interference studies tested normal and neutropenic E. coli murine survival and intraperitoneal lavage bacterial counts. Results D-methionine conferred significant ABR threshold shift reductions. OHC protection was less robust but significant at 20 kHz in the 420 mg/kg/day group. In vitro studies did not detect D-methionine-induced antimicrobial interference. In vivo studies did not detect D-methionine-induced interference in normal or neutropenic mice. Conclusions D-methionine protects from tobramycin-induced ototoxicity without antimicrobial interference. The study results suggest D-met as a potential otoprotectant from clinical tobramycin use in cystic fibrosis patients.

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Section: Discussionmentioning

confidence: 99%

d -Methionine reduces tobramycin-induced ototoxicity without antimicrobial interference in animal models

Fox

Cooper

Speil

et al. 2016

Journal of Cystic Fibrosis

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“…Under certain physiological and pathological conditions, however, its systemic use is ototoxic and nephrotoxic, and thus it cannot be administered to young or dehydrated animals and to patients with renal insufficiency. In the companion animal practice, gentamicin is often administered topically in the form of suspensions, creams, ointments or ear drops, if the tympanic membrane is still intact (Parravicini et al, 1982;Oghan et al, 2011). Direct contact with the inner ear at high local concentrations can result in severe irreversible ototoxic effects (El Bakri et al, 1998).…”

Section: Abstract: Gentamicin Dmso Ipec-j2 Permeationmentioning

confidence: 99%

Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

Gyetvai

Jerzsele

Pászti-Gere

et al. 2015

Acta Veterinaria Hungarica

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Gentamicin is an aminoglycoside antibiotic widely used in combination with dimethyl sulphoxide (DMSO) in topical drug formulations. It is not known, however, whether DMSO can enhance the permeation of gentamicin through biological membranes, leading to oto-and nephrotoxic side effects. A simple and reliable high-performance liquid chromatographic (HPLC) method was applied for the quantitative determination of gentamicin collected from the apical and basolateral compartments of the porcine intestinal epithelial cell line IPEC-J2 cell monolayer using fluorometric derivatisation of the analyte with fluorenylmethyloxycarbonyl chloride (FMOC) prior to chromatographic run in the presence and absence of 1% DMSO. The lack of change in transepithelial electrical resistance (TER) demonstrated that gentamicin and 1% DMSO did not affect IPEC-J2 cell monolayer integrity via the disruption of cell membranes. Chromatographic data also ascertained that gentamicin penetration across the cell monolayer even in the presence of 1% DMSO was negligible at 6 h after the beginning of apical gentamicin administration. This study further indicates that the addition of this organic solvent does not increase the incidence of toxic effects related to gentamicin permeation.

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“…After almost eight decades in clinical use, although the ototoxic effects of aminoglycosides in animals and humans are well documented and also high occurrence risk of another serious complication such as nephrotoxicity, this class of drugs seems still likely to remain an important component of medical treatment options due to: 1) the effectiveness at the treatment of gramnegative aerobic bacterial infections, 2) being an alternative medication among the rare options of treatment in tuberculosis, 3) beneficial effects in Meniere's disease and 4) relatively low cost [1][2][3][4][5][6]. Aminoglycoside antibiotics may injure the outer hair cells at the basal turn of the cochlea and cause sensorineural hearing loss.…”

Section: Introductionmentioning

confidence: 99%

“…Aminoglycoside antibiotics may injure the outer hair cells at the basal turn of the cochlea and cause sensorineural hearing loss. In more severe cases, the damage may progress to inner hair cells and supporting cells [4][5][6]. Therefore, it is important to identify the development mechanism and subsequently prevention methods against to vestibulotoxicity and cochleotoxicity.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Hyperbaric Oxygen Therapy Against Streptomycin Ototoxicity

Bakır¹,

Özbay²,

Kınış³

et al. 2014

Ann Clin Anal Med

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ÖzetAmaç: Hiperbarik oksijen (HBO), önemli antioksidan aktivitesi nedeniyle çe-şitli hastalıkların tedavisinde giderek artan oranda kullanılmakta olan bir adjuvan tedavi metodudur. Bu deneysel çalışmada streptomisine bağlı ototoksisitenin önlenmesinde HBO tedavisinin etkinliğinin araştırılması amaçlan-mıştır. Gereç ve Yöntem: Yirmi sekiz adet erişkin Wistar albino rat randomize olarak 4 gruba ayrıldı: Kontrol (n=7), streptomisin (n=7), HBO (n=7) ve streptomisin+HBO (n=7). HBO uygulanan sıçanlar basınçlı tank içine yerleş-tirildi ve yedi günlük bir süre içinde günde 60 dakika boyunca 2.5 mutlak atmosfer basınçta % 100 oksijen almaları sağlandı. Sıçanların işitme düzeyle-ri HBO uygulaması başlamadan önce ve son gün distortion product otoakustik emisyon (DPOAE) ile test edildi. 7. günde son DPOAE ölçümleri takiben genel anestezi altında tüm gruplardaki hayvanlar sakrifiye edildi. DPOAE amplitüdlerindeki farklılıklar belirlenerek işitme sonuçları istatistiksel olarak analiz edildi. Ayrıca, her sıçanın kokleası hematoksilen ve eozin (H&E) ile histopatolojik olarak ışık mikroskobu altında değerlendirildi. Bulgular: Kontrol ve HBO grubunda histopatolojik değerlendirmede koklear tüylü hücrelerin ço-ğunlukla korunduğu gözlendi. DPOAE sonuçlarında ise tedavi öncesi ve sonrası arasında anlamlı bir fark saptanmadı (p>0.05). Öte yandan, streptomisin ve streptomisin+HBO uygulanan sıçanlarda hem tüylü hücrelerde hemde işitme fonksiyonlarında kayıp saptandı (p<0.05). Streptomisin+HBO grubunun DPgram sonuçları, streptomisin grubunun DPgram sonuçları ile karşılaş-tırıldığında anlamlılık yoktu (p>0.05). Tartışma: Antioksidan özellikli HBO'nun koklear tüylü hücreler üzerinde zararlı bir etkisi gözlenmemiştir. Öte yandan, sıçanlarda oluşturulan streptomisine bağlı koklear hasara karşı da koruyucu bir etkisi olmadığı sonucuna varılmıştır. Anahtar KelimelerAminoglikozid; Streptomisin; Ototoksisite; İşitme Kaybı; Hiperbarik Oksijen Abstract Aim: Hyperbaric oxygen (HBO) is an important adjuvant therapy and being increasingly used in the treatment of various disorders because of having an important antioxidant activity. This experimental study was designed to determine the possible protective effect of HBO therapy on streptomycininduced ototoxicity. Material and Method: Twenty-eight adult Wistar albino rats were divided into four groups: Streptomycin (n=7), saline (n=7), HBO (n=7), and streptomycin plus HBO (n=7). The HBO administered rats were placed into a large pressure chamber and received 100% oxygene at 2.5 atmosphere absolute for 60 minutes per day in a period of seven days. Rats were tested with DPOAE (Distortion Product Otoacoustic Emissions) in the beginning and the end of study. The animals in all groups were sacrificed under general anesthesia on the seventh day. Biopsy specimens from inner ear were stored for histopathologic examination with hematoxylin and eosin (H&E) under light microscopy. Results: Outer hair cells shown by light microscopic images were mostly preserved in control and HBO group. DPOAE measurements reveale...

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Ototoxicity caused by topical administration of gentamicin versus tobramycin in rabbits

Cited by 11 publications

References 14 publications

d -Methionine reduces tobramycin-induced ototoxicity without antimicrobial interference in animal models

d -Methionine reduces tobramycin-induced ototoxicity without antimicrobial interference in animal models

Gentamicin sulphate permeation through porcine intestinal epithelial cell monolayer

The Effects of Hyperbaric Oxygen Therapy Against Streptomycin Ototoxicity

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