2023
DOI: 10.1002/ece3.10038
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Otterly delicious: Spatiotemporal variation in the diet of a recovering population of Eurasian otters (Lutra lutra) revealed throughDNAmetabarcoding and morphological analysis of prey remains

Abstract: Eurasian otters are apex predators of freshwater ecosystems and a recovering species across much of their European range; investigating the dietary variation of this predator over time and space, therefore, provides opportunities to identify changes in freshwater trophic interactions and factors influencing the conservation of otter populations. Here we sampled feces from 300 dead otters across England and Wales between 2007 and 2016, conducting both morphological analyses of prey remains and dietary DNA metab… Show more

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Cited by 7 publications
(5 citation statements)
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“…DNA barcoding with species‐specific COI primers has been used to detect rare crayfishes via eDNA (e.g., Cowart et al., 2018 ; Trujillo‐Gonzalez et al., 2021 ). While this is likely to continue being reliable for species detection, provided primer design was done with adequate reference sequences, we urge caution when using “universal” invertebrate COI primers such as those used in metabarcoding studies (e.g., Drake et al., 2023 ). Our results suggest that unreliable results are likely when simply implementing BLAST searches and adopting a sequence similarity threshold for molecular taxonomic identification that has not been vetted for the genus at hand.…”
Section: Discussionmentioning
confidence: 99%
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“…DNA barcoding with species‐specific COI primers has been used to detect rare crayfishes via eDNA (e.g., Cowart et al., 2018 ; Trujillo‐Gonzalez et al., 2021 ). While this is likely to continue being reliable for species detection, provided primer design was done with adequate reference sequences, we urge caution when using “universal” invertebrate COI primers such as those used in metabarcoding studies (e.g., Drake et al., 2023 ). Our results suggest that unreliable results are likely when simply implementing BLAST searches and adopting a sequence similarity threshold for molecular taxonomic identification that has not been vetted for the genus at hand.…”
Section: Discussionmentioning
confidence: 99%
“…Crayfish species descriptions and delimitation analyses have also used COI barcodes (e.g., Amador et al., 2021 ; Perkins et al., 2023 ). Targeted assessments using eDNA for both rare (e.g., Boyd et al., 2020 ; Quebedeaux et al., 2023 ) and invasive (e.g., Dougherty et al., 2016 ; Geerts et al., 2018 ) species have been successful applications of DNA barcoding, and in the context of crayfish, as have metabarcoding studies (e.g., Drake et al., 2023 ; Kataoka et al., 2022 ). Despite the relatively common use of DNA barcoding in crayfish studies, to date, an overall assessment of local and global barcoding gaps across multiple genera has not been conducted.…”
Section: Introductionmentioning
confidence: 99%
“…DNA metabarcoding, which combines high‐throughput sequencing (HTS) with DNA barcoding, allows for the processing of a greater number of samples and the recognition of multiple taxa in a shorter amount of time (Drake et al., 2023; Kuang et al., 2023). Because of its advantages, DNA metabarcoding has been used in dietary studies of a variety of animals, including sharks (Olin et al., 2023), Lutra lutra (Drake et al., 2023), birds (Schumm et al., 2023) and others. Accordingly, DNA metabarcoding of faecal samples has been used to characterize the plant diet of primates.…”
Section: Introductionmentioning
confidence: 99%
“…DNA metabarcoding, which combines high-throughput sequencing (HTS) with DNA barcoding, allows for the processing of a greater number of samples and the recognition of multiple taxa in a shorter amount of time (Drake et al, 2023;Kuang et al, 2023).…”
Section: Introductionmentioning
confidence: 99%
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