Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Summer, Kate; Browne, Jessica; Hollanders, Matthijs; Benkendorff, Kirsten

doi:10.1016/j.bioflm.2022.100081

Cited by 18 publications

(9 citation statements)

References 105 publications

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“…These experiments revealed that the addition of 1% (v/v) DMSO and 0.1% (v/v) ethanol (equivalent to 17 mM) had a significant impact on AOM, resulting in >70% inhibition of AOM (Figure ). This was a surprising finding considering that DSMO is generally accepted as nontoxic below 10% (v/v) and biological effects of applied concentrations below 2% (v/v) are often unreported as assumed negligible . For comparison, a study on soil bacterial communities found only minor effects of DMSO in concentrations up to 5% (v/v) with a reduction in the growth rate of up to 20% .…”

Section: Resultsmentioning

confidence: 98%

“…This was a surprising finding considering that DSMO is generally accepted as nontoxic below 10% (v/v) and biological effects of applied concentrations below 2% (v/v) are often unreported as assumed negligible. 18 For comparison, a study on soil bacterial communities found only minor effects of DMSO in concentrations up to 5% (v/v) with a reduction in the growth rate of up to 20%. 19 Ethanol, even at low concentrations (0.63 mM), showed an almost complete inhibition of AOM (96 ± 1%) that did not recover within 96 h, which should be sufficient time for the ethanol to be consumed by the side community.…”

Section: Resultsmentioning

confidence: 99%

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Probing Denitrifying Anaerobic Methane Oxidation via Antimicrobial Intervention: Implications for Innovative Wastewater Management

Wissink,

Glodowska,

van der Kolk

et al. 2024

Environ. Sci. Technol.

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Methane emissions present a significant environmental challenge in both natural and engineered aquatic environments. Denitrifying anaerobic methane oxidation (N-DAMO) has the potential for application in wastewater treatment plants. However, our understanding of the N-DAMO process is primarily based on studies conducted on environmental samples or enrichment cultures using metagenomic approaches. To gain deeper insights into N-DAMO, we used antimicrobial compounds to study the function and physiology of 'Candidatus Methanoperedens nitroreducens' and 'Candidatus Methylomirabilis oxyfera' in N-DAMO enrichment cultures. We explored the effects of inhibitors and antibiotics and investigated the potential application of N-DAMO in wastewater contaminated with ammonium and heavy metals. Our results showed that 'Ca. M. nitroreducens' was susceptible to puromycin and 2-bromoethanesulfonate, while the novel methanogen inhibitor 3-nitrooxypropanol had no effect on N-DAMO. Furthermore, 'Ca. M. oxyfera' was shown to be susceptible to the particulate methane monooxygenase inhibitor 1,7-octadiyne and a bacteria-suppressing antibiotic cocktail. The N-DAMO activity was not affected by ammonium concentrations below 10 mM. Finally, the N-DAMO community appeared to be remarkably resistant to lead (Pb) but susceptible to nickel (Ni) and cadmium (Cd). This study provides insights into microbial functions in N-DAMO communities, facilitating further investigation of their application in methanogenic, nitrogen-polluted water systems.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Probing Denitrifying Anaerobic Methane Oxidation via Antimicrobial Intervention: Implications for Innovative Wastewater Management

Wissink,

Glodowska,

van der Kolk

et al. 2024

Environ. Sci. Technol.

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“…DMSO concentrations below 10 % (v/v) are generally considered non‐toxic [68] . A final concentration of 10 % DMSO was used as a negative (solvent) control in all simultaneous experiments [69] .…”

Section: Methodsmentioning

confidence: 99%

“…[67] DMSO concentrations below 10 % (v/v) are generally considered non-toxic. [68] A final concentration of 10 % DMSO was used as a negative (solvent) control in all simultaneous experiments. [69] However, 10 μg/plate sodium azide (TA97a), 40 μg/plate 9-AA (TA98), and 2.5 μg/plate NPDA (TA100) were used as positive controls.…”

Section: Mutagenicity and Antimutagenicity Assaysmentioning

confidence: 99%

In Vitro Genotoxic and Antigenotoxic Effects of Ten Novel Synthesized 4‐Thiazolidinone Derivatives

Alaylar,

Güllüce,

Turhan

et al. 2023

Chemistry & Biodiversity

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Heterocyclic compounds are found in a variety of drug molecules, and bioactive natural products. 4‐Thiazolidinones (4‐TZDs), which represent an important class of heterocyclic compounds, are of great interest today with their diverse bioactivities. In this study, ten novel 4‐TZD derivatives (C1–C10) were synthesized, characterized by spectroscopic techniques, and their genotoxic, and antigenotoxic properties were investigated in vitro using the Ames Salmonella/microsome mutagenicity assay in the concentration range of 0.2–1.0 mM/plate. The results revealed that none of the compounds were mutagenic on the three different Salmonella typhimurium strains up to the highest concentration tested. Furthermore, in our study, C1, C4, C6, and C9 showed significant, ranging from moderate to strong, antigenotoxic effects against mutagen‐induced DNA damage at relatively higher doses. Among these, C4 had the best potential to inhibit the number of revertant colonies induced by 9‐aminoacridine (9‐AA), with a maximum inhibition rate of 47.9 % for 1.0 mM/plate. As a result, preliminary knowledge about the safety of the use of ten novel synthesized 4‐TZD compounds likely to exhibit many bioactivities was obtained in this study. In addition, the significant in vitro antimutagenic activity of some derivatives increases the importance of studies for the development of new pharmacological agents for cancer prevention.

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“…Such minimum reporting information standards for experiments have included where sessile microbial communities exist on surfaces [ 16 ] and experiments that report spectrophotometric and fluorometric methods to assess biofilm formation [ 17 ]. In addition, recent papers have called for further work to standardise such methodology in biofilm research [ 18 , 19 ]. It is also widely acknowledged that vocabulary used to describe biofilms can be inconsistent.…”

Section: Introductionmentioning

confidence: 99%

Critical analysis of methods to determine growth, control and analysis of biofilms for potential non-submerged antibiofilm surfaces and coatings

Redfern,

Cunliffe,

Goeres

et al. 2024

Biofilm

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Out of control: The need for standardised solvent approaches and data reporting in antibiofilm assays incorporating dimethyl-sulfoxide (DMSO)

Cited by 18 publications

References 105 publications

Probing Denitrifying Anaerobic Methane Oxidation via Antimicrobial Intervention: Implications for Innovative Wastewater Management

Probing Denitrifying Anaerobic Methane Oxidation via Antimicrobial Intervention: Implications for Innovative Wastewater Management

In Vitro Genotoxic and Antigenotoxic Effects of Ten Novel Synthesized 4‐Thiazolidinone Derivatives

Critical analysis of methods to determine growth, control and analysis of biofilms for potential non-submerged antibiofilm surfaces and coatings

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