Outbreak of betanodavirus infection in tilapia, <i>Oreochromis niloticus</i> (L.), in fresh water

Bigarré, Laurent; Cabon, Jean-Yves; Baud, M.; Heimann, Marianne; Body, A; Lieffrig, François; Castric, J.

doi:10.1111/j.1365-2761.2009.01037.x

Cited by 94 publications

(70 citation statements)

References 17 publications

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“…In 2007, a betanodavirus was detected by RT-PCR in Nile tilapia larvae following a mass mortality event at a western European fish farm (Bigarré et al 2009), andEyngor et al (2014) isolated a novel RNA virus (tilapia lake virus) from wild tilapia and confirmed its pathogenicity via experimental infection in Nile tilapia.…”

Section: Introductionmentioning

confidence: 99%

Megalocytivirus infection in cultured Nile tilapia Oreochromis niloticus

Subramaniam

Gotesman²,

Smith³

et al. 2016

Dis. Aquat. Org.

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Megalocytiviruses, such as infectious spleen and kidney necrosis virus (ISKNV), induce lethal systemic diseases in both ornamental and food fish species. In this study, we investigated an epizootic affecting Nile tilapia Oreochromis niloticus cultured in the US Midwest. Diseased fish displayed lethargy, gill pallor, and distension of the coelomic cavity due to ascites. Histopathological examination revealed a severe systemic abundance of intravascular megalocytes that were especially prominent in the gills, kidney, spleen, liver, and intestinal submucosa. Transmission electron microscopic examination revealed abundant intracytoplasmic polygonal virions consistent with iridovirus infection. Comparison of the full-length major capsid protein nucleotide sequences from a recent outbreak with a remarkably similar case that occurred at the same facility many years earlier revealed that both epizootics were caused by ISKNV. A comparison of this case with previous reports suggests that ISKNV may represent a greater threat to tilapia aquaculture than previously realized. KEY WORDS: Iridoviridae · Megalocytivirus · Infectious spleen and kidney necrosis virus · Aquaculture · Ornamental fish Resale or republication not permitted without written consent of the publisherDis Aquat Org 119: [253][254][255][256][257][258] 2016 Although tilapia are considered relatively resistant to disease, they are susceptible to common aquatic pathogens (e.g. viruses, bacteria, fungi, water molds, parasites) when reared intensively. The primary microbial pathogens affecting cultivated tilapia include Gram-negative bacteria (Aeromonas hydrophila, Flavobacterium columnare, and Francisella noatunensis), Gram-positive bacteria (Streptococcus spp.), and water molds such as members of the family Saprolegniaceae (e.g. Achyla and Saprolgenia spp.; Plumb & Hanson 2010). Despite the global culture of tilapia, few viruses have been described from tilapia, and none has proven to be a serious threat to the industry (El-Sayed 2006).The first RNA virus discovered in tilapia was an aquabirnavirus isolated from apparently healthy Mozambique tilapia O. mossambicus cultured in Lukang, Taiwan (Hedrick et al. 1983). In 2007, a betanodavirus was detected by RT-PCR in Nile tilapia larvae following a mass mortality event at a western European fish farm (Bigarré et al. 2009), andEyngor et al. (2014) isolated a novel RNA virus (tilapia lake virus) from wild tilapia and confirmed its pathogenicity via experimental infection in Nile tilapia.The first DNA viruses discovered in tilapia were iridoviruses. Although the family Iridoviridae is composed of 5 genera, only members of the genera Megalocytivirus, Lymphocystivirus, and Ranavirus infect fish (Zhang & Gui 2015). Paperna (1973) detected lymphocystis virus in tilapine and haplochromine cichlids from the Rift Valley Lakes Kitangiri and Victoria. A severe (100% mortality) epizootic among Mozambique tilapia fry held in an Australian aquatic disease laboratory was tentatively attributed to a ranavirus, Bohle ir...

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Section: Introductionmentioning

confidence: 99%

Megalocytivirus infection in cultured Nile tilapia Oreochromis niloticus

Subramaniam

Gotesman²,

Smith³

et al. 2016

Dis. Aquat. Org.

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“…Common ectoparasites and the few bacterial pathogens of tilapines are well controlled by pharmacotherapy. Few viral diseases have been reported for tilapia, and these are of limited impact (7)(8)(9).…”

mentioning

confidence: 99%

Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

Tsofack

Zamostiano

Watted³

et al. 2017

J Clin Microbiol

131

115

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Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ponds in Israel and Ecuador. We have identified the etiological agent of these mass die-offs as a novel orthomyxo-like virus and named it tilapia lake virus (TiLV). Here, we provide the conditions for efficient isolation, culturing, and quantification of the virus, including the use of susceptible fish cell lines. Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish organs. This assay revealed, for the first time to our knowledge, the presence of TiLV in diseased Colombian tilapia, indicating a wider distribution of this emerging pathogen and stressing the risk that TiLV poses for the global tilapia industry. Overall, the described procedures should provide the tilapia aquaculture industry with important tools for the detection and containment of this pathogen.

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“…Betanoda virus ini menyerang larva ikan nila ukuran 0-7 setelah menetas (post-hatching larvae) dengan gejala adanya kerusakan jaringan pada bagian otak berupa vakuolasi (Bigarre et al, 2009 Jurnal Riset Akuakultur, 13 (1), 2018, 85-92 …”

Section: Bp Bpunclassified

STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

Koesharyani¹,

Gardenia

Widowati

et al. 2018

Jurnal Riset Akuakultur

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ABSTRAKIkan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1). KATA KUNCI: nila; TiLV; seminested RT-PCR ABSTRACT:Case study of tilapia lake virus (TiLV) infection on nila (Oreochromis niloticus

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Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water

Cited by 94 publications

References 17 publications

Megalocytivirus infection in cultured Nile tilapia Oreochromis niloticus

Megalocytivirus infection in cultured Nile tilapia Oreochromis niloticus

Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

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