Outbreak of Keratomycosis Attributable to Fusarium solani in the French West Indies

Donnio, A; Nuoi, Delphine N.’Guyen Van; Catanèse, M.; Desbois, N.; Ayeboua, L.; Merle, H.

doi:10.1016/j.ajo.2006.09.021

Cited by 33 publications

(18 citation statements)

References 3 publications

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“…The fact that the six most common STs found in drains, representing 70% of the fusaria recovered, are the same six most common STs responsible for human infections strongly supports the hypothesis that plumbing surface biofilms serve as reservoirs for human pathogenic fusaria (2,3,8,32). However, while we hypothesize that STs of opportunistic fusaria are very common in our indoor environment, we are quick to point out that Fusarium infections are relatively rare, even among the severely immunocompromised and immunosuppressed (31,35,48 (13,15,41). It is noteworthy that five of the six most common STs we isolated from sinks represented 54% of the 67 isolates analyzed from infected corneas (i.e., FSSC 1-a, n ϭ 13; FSSC 1-b, n ϭ 3; FSSC 2-d, n ϭ 9; FOSC 33, n ϭ 10; and F. dimerum a, n ϭ 24).…”

Section: Discussionsupporting

confidence: 62%

Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains

et al. 2011

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It has been proposed that plumbing systems might serve as a significant environmental reservoir of human-pathogenic isolates of Fusarium. We tested this hypothesis by performing the first extensive multilocus sequence typing (MLST) survey of plumbing drain-associated Fusarium isolates and comparing the diversity observed to the known diversity of clinical Fusarium isolates. We sampled 471 drains, mostly in bathroom sinks, from 131 buildings in the United States using a swabbing method. We found that 66% of sinks and 80% of buildings surveyed yielded at least one Fusarium culture. A total of 297 isolates of Fusarium collected were subjected to MLST to identify the phylogenetic species and sequence types (STs) of these isolates. Our survey revealed that the six most common STs in sinks were identical to the six most frequently associated with human infections. We speculate that the most prevalent STs, by virtue of their ability to form and grow in biofilms, are well adapted to plumbing systems. Six major Fusarium STs were frequently isolated from plumbing drains within a broad geographic area and were identical to STs frequently associated with human infections.

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Section: Discussionsupporting

confidence: 62%

Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains

et al. 2011

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“…Between November 2005 and May 2006, 10 of 12 keratitis cases in the Caribbean region were associated with the use of RML [40]. Five of the patients had corneal lesions culture positive for F. solani and the one patient who had suffered vegetative trauma to the cornea required penetrating keratoplasty.…”

Section: Outbreak: 2005 á 2006mentioning

confidence: 99%

“…Lens solution bottles tested (n 05) were all culture negative. Prior to January 2004, spanning the years 1969Á1992, less than 10 cases of contact lens associated mycotic keratitis had been reported from the Bascom Palmer Eye Institute in Miami, Florida [22] The above cases of Bernal et al [41], Alfonso et al [22], and Donnio et al [40] were represented in the epidemiological study by Chang et al [23].…”

Section: Outbreak: 2005 á 2006mentioning

confidence: 99%

Fusariumkeratitis and contact lens wear: facts and speculations

et al. 2008

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Over the past several decades mycotic keratitis has been considered a rare sequel to hydrogel contact lens wear. In 2005--2006 an upswing in the incidence of Fusarium keratitis was associated with a disproportionate use of one multipurpose contact lens solution (MPS, ReNu with MoistureLoc, Bausch & Lomb, Rochester, NY). The MPS, as manufactured and marketed, was sterile and met regulatory guidelines for antimicrobial activity. A multivariant interaction of poor hygienic practices and the contact lens paraphernalia were associated with a mostly selective contamination in or on the lens storage case by members of the F. solani/F. oxysporum species complexes from the environment of the user. A decline of the anti-fusaria properties of the MPS in the lens case appeared related to its dissociation from drying, or dilution and the potential for sorption of antimicrobial solution components (e.g., alexidine) to various hydrogel lenses. These factors and capacities of the fusaria for rapid amplification by microcycle conidiation, production of dormant resistant cells, and potential for attachment and penetration of hydrogel lenses, were linked to the occasional selective fungal survival and growth during storage of the lens in MPS. Lack of a manual rubbing-cleaning step in the MPS disinfection process was considered a risk factor for keratitis.

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“…Recently several investigations, including those by the Singapore Health Ministry and the U.S. Centers for Disease Control and Prevention (CDC) and in the French West Indies, reported outbreaks of Fusarium keratitis associated with contact lens wear (16,18,29). Although fungal keratitis is generally associated with trauma and prior application of corticosteroids (6), patients in these outbreaks had no history of recent ocular trauma (16).…”

mentioning

confidence: 99%

FusariumandCandida albicansBiofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions

Imamura¹,

Chandra²,

Mukherjee³

et al. 2008

Antimicrob Agents Chemother

199

134

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Fungal keratitis is commonly caused by Fusarium species and less commonly by Candida species. Recent outbreaks of Fusarium keratitis were associated with contact lens wear and with ReNu with MoistureLoc contact lens care solution, and biofilm formation on contact lens/lens cases was proposed to play a role in this outbreak. However, no in vitro model for contact lens-associated fungal biofilm has been developed. In this study, we developed and characterized in vitro models of biofilm formation on various soft contact lenses using three species of Fusarium and Candida albicans. The contact lenses tested were etafilcon A, galyfilcon A, lotrafilcon A, balafilcon A, alphafilcon A, and polymacon. Our results showed that clinical isolates of Fusarium and C. albicans formed biofilms on all types of lenses tested and that the biofilm architecture varied with the lens type. Moreover, differences in hyphal content and architecture were found between the biofilms formed by these fungi. We also found that two recently isolated keratitis-associated fusaria formed robust biofilms, while the reference ATCC 36031 strain (recommended by the International Organization for Standardization guidelines for testing of disinfectants) failed to form biofilm. Furthermore, using the developed in vitro biofilm model, we showed that phylogenetically diverse planktonic fusaria and Candida were susceptible to MoistureLoc and MultiPlus. However, Fusarium biofilms exhibited reduced susceptibility against these solutions in a species-and time-dependent manner. This in vitro model should provide a better understanding of the biology and pathogenesis of lens-related fungal keratitis.

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Outbreak of Keratomycosis Attributable to Fusarium solani in the French West Indies

Cited by 33 publications

References 3 publications

Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains

Widespread Occurrence of Diverse Human Pathogenic Types of the Fungus Fusarium Detected in Plumbing Drains

Fusariumkeratitis and contact lens wear: facts and speculations

FusariumandCandida albicansBiofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions

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