Outbreak of PER-1 and diversity of β-lactamases among ceftazidime-resistant Pseudomonas aeruginosa clinical isolates

Qing, Yun; Chen, Kun; Fang, Zhi-Li; Huang, Ying-Min; Zhang, Xuefei; Tian, Guo‐Bao; Huang, Xi

doi:10.1099/jmm.0.069427-0

Cited by 22 publications

(21 citation statements)

References 41 publications

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“…In this study, none of these β-lactamases were found to be enriched in the manure samples; however, several β-lactamases (the Per-1, Per-2, IMP-2 and IMP-5 groups in the arable soil, whereas Per-1, SFO-1, IMP-2 and IMP-5 groups in the pasture soil), which are common in clinical isolates (e.g. Docuquier et al 2003;Qing et al 2014), were observed to be highly enriched in manure-treated soils (Fig. 2), suggesting that the enrichment was likely due to the stimulation of soil indigenous bacteria harboring β-lactamases induced by manure application.…”

Section: Temporal Patterns Of Soil Args and Stimulation Of β-Lactam-rmentioning

confidence: 99%

Temporal changes of antibiotic-resistance genes and bacterial communities in two contrasting soils treated with cattle manure

Han

Shi

et al. 2015

FEMS Microbiology Ecology

111

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One sentence summary: The study indicates that even in the absence of selective pressure imposed by agricultural use of antibiotics, manure application could still strongly impact the abundance, diversity and mobility potential of a broad spectrum of soil ARGs. Editor: Kornelia Smalla ABSTRACTThe emerging environmental spread of antibiotic-resistance genes (ARGs) and their subsequent acquisition by clinically relevant microorganisms is a major threat to public health. Animal manure has been recognized as an important reservoir of ARGs; however, the dissemination of manure-derived ARGs and the impacts of manure application on the soil resistome remain obscure. Here, we conducted a microcosm study to assess the temporal succession of total bacteria and a broad spectrum of ARGs in two contrasting soils following manure application from cattle that had not been treated with antibiotics. High-capacity quantitative PCR detected 52 unique ARGs across all the samples, with β-lactamase as the most dominant ARG type. Several genes of soil indigenous bacteria conferring resistance to β-lactam, which could not be detected in manure, were found to be highly enriched in manure-treated soils, and the level of enrichment was maintained over the entire course of 140 days. The enriched β-lactam resistance genes had significantly positive relationships with the relative abundance of the integrase intI1 gene, suggesting an increasing mobility potential in manure-treated soils. The changes in ARG patterns were accompanied by a significant effect of cattle manure on the total bacterial community compositions. Our study indicates that even in the absence of selective pressure imposed by agricultural use of antibiotics, manure application could still strongly impact the abundance, diversity and mobility potential of a broad spectrum of soil ARGs. Our findings are important for reliable prediction of ARG behaviors in soil environment and development of appropriate strategies to minimize their dissemination.

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Section: Temporal Patterns Of Soil Args and Stimulation Of β-Lactam-rmentioning

confidence: 99%

Temporal changes of antibiotic-resistance genes and bacterial communities in two contrasting soils treated with cattle manure

Han

Shi

et al. 2015

FEMS Microbiology Ecology

111

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“…National surveys from Turkey then showed that PER-1-producing P. aeruginosa isolates are widespread in Turkey [2]. PER-1 has been reported in European countries with no geographical border with Turkey, such as Belgium [43], Italy [44], Spain [45], Poland [46], Hungary and Serbia [47] and Tunisia [48] as well as in Asian countries [49,50]. In addition, it was identified in Greece and Iran [51,52] (Table 1).…”

Section: Extended-spectrum ˇ-Lactamases (Esbls)mentioning

confidence: 99%

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii : Mechanisms and epidemiology

Potron

Poirel

Nordmann

2015

International Journal of Antimicrobial Agents

604

460

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Multidrug resistance is quite common among non-fermenting Gram-negative rods, in particular among clinically relevant species including Pseudomonas aeruginosa and Acinetobacter baumannii. These bacterial species, which are mainly nosocomial pathogens, possess a diversity of resistance mechanisms that may lead to multidrug or even pandrug resistance. Extended-spectrum ␤-lactamases (ESBLs) conferring resistance to broad-spectrum cephalosporins, carbapenemases conferring resistance to carbapenems, and 16S rRNA methylases conferring resistance to all clinically relevant aminoglycosides are the most important causes of concern. Concomitant resistance to fluoroquinolones, polymyxins (colistin) and tigecycline may lead to pandrug resistance. The most important mechanisms of resistance in P. aeruginosa and A. baumannii and their most recent dissemination worldwide are detailed here.

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“…Clinical interest in PER-1 has increased recently due to the detection of this enzyme in multiple Pseudomonas aeruginosa isolates in hospitals in Asia (188), in isolates of Acinetobacter baumannii in Kuwait (189), and in isolates of Providencia stuartii in Tunisia (190).…”

Section: Structure-function Relationships Of Class a Enzymesmentioning

confidence: 99%

A Structure-Based Classification of Class A β-Lactamases, a Broadly Diverse Family of Enzymes

Slama²,

et al. 2016

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SUMMARYFor medical biologists, sequencing has become a commonplace technique to support diagnosis. Rapid changes in this field have led to the generation of large amounts of data, which are not always correctly listed in databases. This is particularly true for data concerning class A β-lactamases, a group of key antibiotic resistance enzymes produced by bacteria. Many genomes have been reported to contain putative β-lactamase genes, which can be compared with representative types. We analyzed several hundred amino acid sequences of class A β-lactamase enzymes for phylogenic relationships, the presence of specific residues, and cluster patterns. A clear distinction was first made between dd-peptidases and class A enzymes based on a small number of residues (S70, K73, P107, 130SDN132, G144, E166, 234K/R, 235T/S, and 236G [Ambler numbering]). Other residues clearly separated two main branches, which we named subclasses A1 and A2. Various clusters were identified on the major branch (subclass A1) on the basis of signature residues associated with catalytic properties (e.g., limited-spectrum β-lactamases, extended-spectrum β-lactamases, and carbapenemases). For subclass A2 enzymes (e.g., CfxA, CIA-1, CME-1, PER-1, and VEB-1), 43 conserved residues were characterized, and several significant insertions were detected. This diversity in the amino acid sequences of β-lactamases must be taken into account to ensure that new enzymes are accurately identified. However, with the exception of PER types, this diversity is poorly represented in existing X-ray crystallographic data.

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Outbreak of PER-1 and diversity of β-lactamases among ceftazidime-resistant Pseudomonas aeruginosa clinical isolates

Cited by 22 publications

References 41 publications

Temporal changes of antibiotic-resistance genes and bacterial communities in two contrasting soils treated with cattle manure

Temporal changes of antibiotic-resistance genes and bacterial communities in two contrasting soils treated with cattle manure

Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii : Mechanisms and epidemiology

A Structure-Based Classification of Class A β-Lactamases, a Broadly Diverse Family of Enzymes

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