Outbreaks of Peste Des Petits Ruminants (PPR) in Goats in Punjab, India

jan, Maha; Filia, Gursimran; Bal, Madhusmita; Kaur, Gurpreet; Sharma, Savitri; Dantotia, A.; Singh, CB

doi:10.20546/ijcmas.2017.608.447

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…For diagnosis of PPRV infection, non-invasive field samples taken from live infected animals like ocular discharges, nasal secretions, mouth and gum erosions samples, rectal mucosae, feacal samples and anticoagulant-treated blood provide a valuable source of viral RNA, are easily obtained, easily transported to the laboratory and are ideal for endemic countries since mortality rates are still low and postmortem tissue samples may be not available yet. While lymph nodes (mesenteric and bronchial), spleen, brain, lungs, skin and intestinal mucosae obtained from necropsied animals are recommended [38,39]. Controversial results obtained by many authors, ŞEVİK [39] obtained positive results in all samples of liver, lung, lymph nodes and spleen using conventional and real time RT-PCR targeting the F and N gene, respectively, and he concluded that these samples can equally be used for viral RNAdetection using these assays.…”

Section: Discussionmentioning

confidence: 99%

Virological Diagnoses of Pesti des petitis Ruminants Virus in Sheep in Giza Governorate

Ali

Hosny²,

Mahmoud

2021

NIDOC-ASRT

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P ESTE des petits ruminants (PPR) is an intense and extremely contagious disease with high mortality in small ruminants resulting inconsiderable socio-economic impact in developing countries. Our study was established during the routine follow up in El-Giza governorate during 2019. A total number of 45 swabs, 40 sera samples and 15 post-mortem epithelial tissues were collected from sheep showing clinical signs suggestive for PPR.PPRVdetection was investigated using serological, pathological and RT-PCRdiagnosis tests.Serologically, PPRV antigens detection were 86%, 93.3%, 66.63% and 73.3% in the nasal, ocular, faecal swabs and different tissues samples, respectively. While, PPRV antibodies detection were 95% in the collected sera samples.Histopathologically, the lung showed severe bronchiectasis and syncytium formation with high accumulation of inflammatory cells. The liver exhibited necrosis with severe portal and central hypertension filled with inflammatory cells.The kidneys revealed severe atrophied glomeruli with destructed renal glomerular capillaries, hemorrhage in the glomeruli and renal tubules with thrombosis of blood vessels. Spleen revealed proliferation of central arterioles with periarteriolar proliferation of connective tissues and hypercellular red pulp with hemorrhage. The mouth commissures suffering from nodules and ulceration which exhibited microscopically multiple vesicles in the epidermis and dissociation of the epidermal and dermal layers filled with inflammatory cells and eosinophils with syncytium formation. A 448 bp band by RT-PCRwas obtained from all nasal and ocular samples, while 2 lung samples were negative for this test.Therefore, the results proved the presence of the disease in El-Giza governorate.

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Section: Discussionmentioning

confidence: 99%

Virological Diagnoses of Pesti des petitis Ruminants Virus in Sheep in Giza Governorate

Ali

Hosny²,

Mahmoud

2021

NIDOC-ASRT

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Seroprevalence of peste des petits ruminants and bluetongue in goat population of Meghalaya, India

et al. 2018

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Aim:This study aimed to determine the seroprevalence of peste des petits ruminants (PPR) and bluetongue (BT) in goats’ population in the state of Meghalaya of Northeast India.Materials and Methods:The serosurveillance study was done from the random sampling (n=598) of blood collected from five districts (Ri-Bhoi, East Khasi Hills, West Khasi Hills, Jaintia Hills and West Garo Hills) of Meghalaya. The presence of antibodies against PPR and BT in the samples was detected by indirect enzyme-linked immunosorbent assay (ELISA) method for PPR and competitive ELISA for BT.Results:The results showed the overall seropositivity of PPR and BT at 7.19% and 60.20%, respectively. West Garo Hills recorded the highest seroprevalence of both PPR (9.81%) and BT (68%) and 3.6% of the samples tested positive for both PPR and BT.Conclusion:The random survey results indicating the presence of PPR and BT have specific implication in epidemiological perspectives since it highlights the prevalence under natural situations, where the subclinical, inapparent, or non-lethal or recovery of infection was suspected in unvaccinated animals. It also warrants further studies to suggest appropriate control measures to prevent the spread of infection.

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Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

et al. 2020

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Background and Aim: Peste des petits ruminants (PPR) is a contagious, World Organization for Animal Health notifiable, economically important, transboundary morbilliviral disease of sheep and goats. Studying seroprevalence of PPR from different geographical areas under varying agro-climatic conditions may help in formulating effective and appropriate disease control strategies under the ongoing national PPR control program. The present cross-sectional study describes the prevalence of PPR virus antibodies in sheep and goats in the various epidemiological units in different states (Haryana, Himachal Pradesh [HP], Jammu and Kashmir [J&K], Punjab, Uttarakhand [UK], and Uttar Pradesh [UP]) of the northern region of India. Materials and Methods: A total of 5843 serum samples (sheep [n=2463] and goats [n=3380]) were collected by stratified random sampling method from 322 epidemiological units in the studied region during 2017-2018 and tested for PPR virus (PPRV) antibodies by competitive ELISA. Results: The results revealed that an overall seroprevalence of 44.05% (2574/5843) with 57.32%, 55.22%, 65.69%, 37.09%, 32.73%, and 29.35% prevalence of PPRV antibodies in small ruminants in Haryana, Punjab, UP, HP, J&K, and UK states, respectively. Further, Chi-squared test revealed an association of PPRV antibodies in goats (χ2=252.28, p<0.01) and sheep (χ2=192.12, p<0.01) across different states in the region. Conclusion: The seroprevalence in majority of the epidemiological units (n=130) in sheep and goats in the studied region had <30%. This necessitates comprehensive, rigorous, continuous vaccination and active surveillance programs for few more years to achieve the desired 70% seroprevalence level of PPRV antibodies in population and to make the northern region of India, as PPR free zone.

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Outbreaks of Peste Des Petits Ruminants (PPR) in Goats in Punjab, India

Cited by 3 publications

References 8 publications

Virological Diagnoses of Pesti des petitis Ruminants Virus in Sheep in Giza Governorate

Virological Diagnoses of Pesti des petitis Ruminants Virus in Sheep in Giza Governorate

Seroprevalence of peste des petits ruminants and bluetongue in goat population of Meghalaya, India

Seroprevalence study of peste des petits ruminants in sheep and goats in the northern region of India

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