Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia

Karacan, Meriç; Alwaeely, Faiz A; Erkan, Serdar; Cebi, Z.; Berberoglugil, M.; Batukan, Melike; Ulug, M.; Arvas, Ayşe; Çamlibel, T.

doi:10.1016/j.fertnstert.2013.06.031

Cited by 63 publications

(62 citation statements)

References 38 publications

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“…Histopathology has also been shown to be the only reliable predictor of successful TESE Plas et al 1999]. Recent studies reported that, although testicular histopathology is the strongest predictor of sperm retrieval in men with NOA, testicular histopathology does not have any influence on outcome of microdissection TESE for ICSI [Abdel Raheem et al 2013;Karacan et al 2013]. However, TESE performed via an open biopsy is an effective and reliable technique for retrieving spermatozoa, with generally successful spermatozoa recovery rates in patients with NOA [Wood et al 2002;Schwarzer et al 2003;Giorgetti et al 2005].…”

Section: Discussionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were assessed. No statistically significant differences were observed in any of the other measured parameters between the three subgroups of NOA. No significant differences in laboratory outcomes were observed between spermatozoa from fresh and frozen testicular spermatozoa; however, statistically significant differences were observed in the pregnancy and implantation rates between groups (p50.05). The outcomes of using spermatozoa retrieved from fresh testicular tissue in each of the three subgroups were also compared; although clinical outcomes showed low results, no significant differences were observed between the three subgroups. Similarly, no significant differences were observed in spermatozoa retrieved from frozen testicular tissue. Once spermatozoa have been successfully obtained, acceptable laboratory outcomes can be achieved for NOA, whether or not the spermatozoa are cryopreserved. However, satisfactory clinical outcomes may be more difficult to achieve as the results showed in each group of fresh and frozen testicular spermatozoa. Therefore, achieving acceptable clinical pregnancy results and efficient cryopreservation of testicular spermatozoa should be considered in patients with NOA.

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Section: Discussionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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“…Fertilization and pregnancy rates were similar between fresh and frozen thawed sperm in patients with OA and NOA [Karacan et al 2013;Nicopoullos et al 2004]. Thus, it is useful to cryopreserve surgically retrieved sperm samples in patients with OA/NOA as the outcomes are only affected by the type of azoospermia and not by the cryopreservation process itself [Schwarzer et al 2003].…”

Section: Storage Methods For Small Samplesmentioning

confidence: 99%

Effect of sperm storage and selection techniques on sperm parameters

Sharma

Kattoor

Ghulmiyyah

et al. 2014

Systems Biology in Reproductive Medicine

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Sperm cryopreservation preserves the fertility of cancer patients undergoing chemotherapy, ensures sperm are available at the time of oocyte retrieval in assisted reproductive technology (ART) procedures and avoids the need for repeated sperm extraction surgeries in azoospermic patients. Conventional methods of cryopreservation involve storage in liquid nitrogen (LN 2 ), which causes a significant decline in sperm parameters such as motility and viability and results in DNA damage. Newer methods of sperm cryopreservation such as the LN 2 vapor method, vitrification, and experimental methods such as lyophilization, have significant advantages over the conventional methods in terms of cost effectiveness and ease of use. Density gradient centrifugation (DGC), swim up, and magnetic assisted cell sorting (MACS) can be used prior to or post-cryopreservation to improve post-thaw sperm quality. Cryopreservation in special carriers such as cryoloops and empty zona prevents the loss of small numbers of sperm during cryopreservation. This article will discuss these sperm preservation and selection techniques.

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“…In previous studies any difference between fertilization, and clinical pregnancy rates was bnot reported when m-TESE, and ICSI performed with testicular spermatozoa used at the same day or 24 hours after incubation. [18,19] Therefore in our study outcomes of ICSI performed with testicular spermatozoa retrieved one day apart were evaluated in the same group.…”

Section: Discussionmentioning

confidence: 99%

Results of intracytoplasmic sperm injection performed with sperm retrieved by microscopic testicular sperm extraction in azoospermic patients

Erdem¹,

Karacan²,

Cebi³

et al. 2018

Turkish Journal of Urology

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Objective: The absence of any sperm in the ejaculate is called azoospermia and it is detected in 1% of males and 10-15% of those with infertility complaints. Azoospermia may be due to obstructive (OA) and non-obstructive (NOA) causes. Today, healthy pregnancies can be achieved in azoospermic patients by intracytoplasmic sperm injection (ICSI) performed using sperm retrieved from microscopic testicular sperm extraction (m-TESE). In this study, we examined the sperm retrieval rates with m-TESE in azoospermic patients, the results of ICSI in OA and NOA patients with sperm and the underlying testicular pathologies in patients without sperm. Material and methods:Patients who underwent m-TESE at IVF unit of our hospital between January 2005 and April 2017 were retrospectively reviewed. A total of 342 azoospermic patients (117 OA and 225 NOA cases) with regular follow-up were included in the study. In these cases, sperm retrieval and clinical pregnancy rates after ICSI were compared.Results: Mean duration of infertility was 28.2±7.8 months in the OA group, 34.2±5.4 years in patients, 30.3±2.9 years in spouses. In the NOA group, the mean duration of infertility was 30.3±6.5 months, the mean age of the patients was 35.3±3.4, and the mean age of the spouses was 30.6±3.3 years. In 68.9% of the cases, the therapeutic indication was male factor alone, while 31.1% had female factor infertility. In the OA group, 68.9% of the cases had only male factor infertility, while 31.1% of them had also female factor infertility. In the NOA group, 65.4% of the cases had only male factor infertility, and female factor was found in 34.6% of the cases. OA patients had a mean serum FSH level of 11.7±3.7 mIU/mL and mean testicular volume of 12.5±2.6 mL, NOA patients had a mean serum FSH level of 13.7±5.4 mIU/mL and mean testicular volume of 9.8±3.4 mL. In the m-TESE procedure, motile sperm was found in all of the OA patients and in 52.4% (118/225) of the NOA patients. Clinical pregnancy rate in the OA group was 29.9% (35/117) and live birth rate was 25.6% (30/117). In the NOA group, the clinical pregnancy rate was 27.1% (32/118) and the live birth rate was 23.7% (27/118). Histopathologic evaluation was made in 107 cases in the NOA group with no testicular sperm, revealing that 59 cases with germ-cell aplasia (sertoli-cell only syndrome), 42 cases with maturation arrest, and 6 cases with hypospermatogenesis. Postoperative hematoma developed in 3 of m-TESE cases and subsided with conservative treatment. Conclusion:If motile sperm is retrieved with m-TESE application in azoospermic patients, pregnancy resulting in one live birth in about 4 couples who undergo ICSI application can be achieved. In the presence of motile sperm, live birth rates are similar between OA and NOA case with very low complication rates.

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Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia

Cited by 63 publications

References 38 publications

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effect of sperm storage and selection techniques on sperm parameters

Results of intracytoplasmic sperm injection performed with sperm retrieved by microscopic testicular sperm extraction in azoospermic patients

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