Outdoor pilot-scale production of Botryococcus braunii in panel reactors

Bazaes, J.; Sepúlveda, Claudia; Acién, F.G.; Morales, Juan; Gonzales, Leonel; Rivas, Mariella; Riquelme, Carlos

doi:10.1007/s10811-012-9787-3

Cited by 37 publications

(22 citation statements)

References 20 publications

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“…Microalgae Botryococcus braunii race A was supplied by Antofagasta University (Chile), where it was grown in pilot scale batch reactors (Bazaes et al, 2012).…”

Section: Microalgaementioning

confidence: 99%

Anaerobic co-digestion of lipid-spent microalgae with waste activated sludge and glycerol in batch mode

Neumann

Torres-Aravena

Fermoso

et al. 2015

International Biodeterioration & Biodegradation

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Anaerobic co-digestion of lipid-spent Botryococcus braunii (LSBB) with waste activated sludge (WAS) and glycerol was studied. Different co-digestion mixtures were assessed in biochemical methane potential tests, performed at mesophilic temperature (35ºC). The experimental methane yields obtained were compared to theoretical values calculated from the methane individual yields. No significant increase in BMP was observed when mixing these substrates. A first-order exponential kinetic model was used to obtain the apparent kinetic constant of the processes assayed. The kinetic constant value of the mixture 25%WAS-75%LSBB was 116%, 16% and 43% higher than for WAS, LSBB and glycerol alone, respectively.2 Keywords: Lipid spent microalgae; Waste activated sludge; Glycerol; Codigestion; Biomethane. INTRODUCTIONBiodiesel production from microalgae has been regarded as an important advance in the development of biofuels (Scott et al., 2010). However, sustainable biodiesel production from microalgae still faces several limitations. One of them is the energy requirement of the operations involved in the production of biodiesel itself, such as mixing, pumping, biomass harvesting, drying, and lipid extraction. The result is that, when traditional processes and technologies are considered, microalgae based biodiesel presents low or even negative energy yields (Scott et al., 2010). This situation can be addressed, at least partially, by anaerobic digestion of the lipid-spent microalgae. Produced biogas can be used as a source of energy, and released nutrients can be recycled for its use during the cultivation of microalgae (Ehimen et al., 2011;Torres et al., 2013). The study carried out by Lardon et al. (2009) indicates that 1.23 MJ of energy could be recovered from lipid-spent microalgae, per MJ of produced biodiesel. Indeed, Campbell et al. (2011) determined that production of biodiesel and biogas would represent a competitive fuel production scheme.Of the wide variety of available species of microalgae, Botryococcus braunii presents great potential for the production of biofuels, especially biodiesel. This is mainly due to the capacity of this strain to accumulate lipids and hydrocarbons, under appropriate culture conditions. Moreover, an adequate profile of triglycerides for transesterification to biodiesel have been observed, comprising mainly palmitic, oleic 3 and linoleic acids (Sydney et al., 2011;Ashokkumar and Rengasamy, 2012).Botryococcus braunii is also characterized by the high presence of extracellular hydrocarbons, mainly terpenes (Wolf, 1983;Metzger and Largeau, 2005 improve operational parameters such as C/N ratio, or to dilute inhibitors. As a result, a higher process performance can be achieved. Furthermore, co-digestion allows the treatment of different residues using the same installations. Indeed, co-digestion have been proposed as a promising approach for improving the methane yield and overall digestion performance (Fernández-Rodríguez et al., 2014). In this scenario, glycerol may be considere...

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“…Microalgae Botryococcus braunii race A was supplied by Antofagasta University (Chile), where it was grown in pilot scale batch reactors (Bazaes et al, 2012).…”

Section: Microalgaementioning

confidence: 99%

Anaerobic co-digestion of lipid-spent microalgae with waste activated sludge and glycerol in batch mode

Neumann

Torres-Aravena

Fermoso

et al. 2015

International Biodeterioration & Biodegradation

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“…This lipid content was much higher than most of the records in the outdoor cultures, indicating a lipid content of 15.6% for Botryococcus braunii (Bazaes et al, 2012), 19-32% for Chlorella sp (Moheimani, 2012(Moheimani, , 2013, 34.7% for N. oculata and N. salina (Quinn et al, 2012), less than 30% for S. acutus (Doria et al, 2011), and 24-30% for Tetraselmis suecica (Moheimani, 2013). In addition, the lipid productivity averaged at 28.1 mg…”

Section: Abundansmentioning

confidence: 74%

Selection of microalgae for biodiesel production in a scalable outdoor photobioreactor in north China

Xia

Song

et al. 2014

Bioresource Technology

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“…We tested the microalgal biomass of Botryococcus braunii UTEX572 cultured under outdoor batch conditions in UMA2 medium in 0.4-m 3 photobioreactors as described by Bazaes et al (22). Nannochloropsis gaditana CCMP527 was subjected to continuous culture at a concentration of approximately 2 g liter Ϫ1 in F/2 medium (23) for 48 h at 30°C and 125 rpm.…”

Section: Methodsmentioning

confidence: 99%

Use of Cellulolytic Marine Bacteria for Enzymatic Pretreatment in Microalgal Biogas Production

Muñoz

Hidalgo

Zapata

et al. 2014

Appl Environ Microbiol

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101

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In this study, we designed and evaluated a microalgal pretreatment method using cellulolytic bacteria that naturally degrades microalgae in their native habitat. Bacterial strains were isolated from each of two mollusk species in a medium containing 1% carboxymethyl cellulose agar. We selected nine bacterial strains that had endoglucanase activity: five strains from Mytilus chilensis, a Chilean mussel, and four strains from Mesodesma donacium, a clam found in the Southern Pacific. These strains were identified phylogenetically as belonging to the genera Aeromonas, Pseudomonas, Chryseobacterium, and Raoultella. The cellulase-producing capacities of these strains were characterized, and the degradation of cell walls in Botryococcus braunii and Nannochloropsis gaditana was tested with "whole-cell" cellulolytic experiments. Aeromonas bivalvium MA2, Raoultella ornithinolytica MA5, and Aeromonas salmonicida MC25 degraded B. braunii, and R. ornithinolytica MC3 and MA5 degraded N. gaditana. In addition, N. gaditana was pretreated with R. ornithinolytica strains MC3 and MA5 and was then subjected to an anaerobic digestion process, which increased the yield of methane by 140.32% and 158.68%, respectively, over that from nonpretreated microalgae. Therefore, a "whole-cell" cellulolytic pretreatment can increase the performance and efficiency of biogas production.

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Outdoor pilot-scale production of Botryococcus braunii in panel reactors

Cited by 37 publications

References 20 publications

Anaerobic co-digestion of lipid-spent microalgae with waste activated sludge and glycerol in batch mode

Anaerobic co-digestion of lipid-spent microalgae with waste activated sludge and glycerol in batch mode

Selection of microalgae for biodiesel production in a scalable outdoor photobioreactor in north China

Use of Cellulolytic Marine Bacteria for Enzymatic Pretreatment in Microalgal Biogas Production

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