Outdoor Temperature, Age, Sex, Body Mass Index, and Diabetic Status Determine the Prevalence, Mass, and Glucose-Uptake Activity of 18F-FDG-Detected BAT in Humans

Ouellet, V.; Routhier-Labadie, Annick; Bellemare, William; Lakhal‐Chaieb, Lajmi; Turcotte, Éric; Carpentier, André C.; Richard, Denis

doi:10.1210/jc.2010-0989

Cited by 496 publications

(476 citation statements)

References 26 publications

Supporting

Mentioning

437

Contrasting

Unclassified

Order By: Relevance

“…In humans, several studies have recently shown negative correlations between BAT activity and adiposity [3,12,13] and age [14]. A negative association between BAT activity and diabetic status, independent of BMI and age, has also been reported in a retrospective study [15]. In addition, an impaired thermogenic response to mild cold has been found in diabetic women compared with obese women [16], suggesting a role for insulin resistance in mediating these impairments.…”

Section: Introductionmentioning

confidence: 79%

Glucose uptake in human brown adipose tissue is impaired upon fasting-induced insulin resistance

et al. 2014

View full text Add to dashboard Cite

Aims/hypothesis Human brown adipose tissue (BAT) has recently emerged as a potential target in the treatment of type 2 diabetes, owing to its capacity to actively clear glucose from the circulation-at least upon cold exposure. The effects of insulin resistance on the capacity of human BAT to take up glucose are unknown. Prolonged fasting is known to induce insulin resistance in peripheral tissues in order to spare glucose for the brain. Methods We studied the effect of fasting-induced insulin resistance on the capacity of BAT to take up glucose during cold exposure as well as on cold-stimulated thermogenesis. BAT glucose uptake was assessed by means of cold-stimulated dynamic 2-deoxy-2-[ 18 F]fluoro-D-glucose positron emission tomography/computed tomography ([ 18 F]FDG-PET/CT) imaging.Results We show that a 54 h fasting period markedly decreases both cold-induced BAT glucose uptake and nonshivering thermogenesis (NST) during cold stimulation. In vivo molecular imaging and modelling revealed that the reduction of glucose uptake in BAT was due to impaired cellular glucose uptake and not due to decreased supply. Interestingly, decreased BAT glucose uptake upon fasting was related to a decrease in core temperature during cold exposure, pointing towards a role for BAT in maintaining normothermia in humans. Conclusions/interpretation Cold-stimulated glucose uptake in BAT is strongly reduced upon prolonged fasting. When cold-stimulated glucose uptake in BAT is also reduced under other insulin-resistant states, such as diabetes, cold-induced activation of BAT may not be a valid way to improve glucose clearance by BAT under such conditions. Trial registration: www.trialregister.nl NTR3523 Funding: This work was supported by the EU FP7 project DIABAT (HEALTH-F2-2011-278373

show abstract

Section: Introductionmentioning

confidence: 79%

Glucose uptake in human brown adipose tissue is impaired upon fasting-induced insulin resistance

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Cypess et al used the same threshold [16]. However, Vosselman et al used a threshold of 1.5 g/ml [17] and van Marken Lichtenbelt et al reported that they used a 'set threshold' [8], whereas Carey et al and Ouellet et al considered an SUV >1.0 g/ml as BAT [18,19]. The lower the chosen threshold, the higher the observed volume of BAT will be.…”

Section: Discussionmentioning

confidence: 99%

Cold-induced activity of brown adipose tissue in young lean men of South-Asian and European origin

et al. 2013

View full text Add to dashboard Cite

Aims/hypothesis South Asians have a disproportionately high risk of developing abdominal obesity, insulin resistance and type 2 diabetes. Brown adipose tissue (BAT) has been identified as a possible target to fight obesity and protect against metabolic disturbance. We explored whether lower BAT activity in South Asians compared with Europids may contribute to the high risk of metabolic disturbance. Methods We studied 20 healthy men (ten Europids/ten South Asians, BMI 19-25 kg/m 2 , age 18-32 years). Following 2 h of cold exposure (16-18°C) after an overnight fast, 18 F-fluorodeoxyglucose ( 18 F-FDG) positronemission tomography-computed tomography (CT) and 123 I-metaiodobenzylguanidine ( 123 I-MIBG) single-photon emission computed tomography-CT were performed to visualise metabolic BAT activity and sympathetic stimulation of BAT. Metabolic BAT activity was defined as maximal standardised uptake value (SUV max ) of 18 F-FDG, and sympathetic stimulation of BAT as semiquantitative uptake value (SQUV) of 123 I-MIBG. We performed hyperinsulinaemic-euglycaemic clamps to assess insulin sensitivity. Spearman's correlations for SUV max of 18 F-FDG and both SQUV of 123 I-MIBG and insulin sensitivity were determined. Results The median (interquartile range) SUV max of 18 F-FDG in South Asians (7.5 [2.2-10.6] g/ml) was not different from the median SUV max obtained in Europids (4.5 [2.2-8.4] g/ml; p=0.59). There was no correlation between BAT activity and insulin sensitivity. Correlations between SQUV of 123 I-MIBG and SUV max of 18 F-FDG were positive, both in the total population (ρ=0.80, p<0.001) and after stratification by ethnicity (Europids, ρ =0.65, p = 0.04; South Asians, ρ=0.83, p=0.01). Conclusions/interpretation This is the first study to prospectively investigate ethnic differences in metabolic BAT activity during cold exposure. We did not find differences in BAT activity between South Asians and Europids. Therefore, it seems unlikely that BAT plays an important role in the development of unfavourable metabolic profiles in South Asians.

show abstract

“…Since previous studies have indicated that BAT activity varies seasonally [4,25], we conducted trials for each individual within a 14 day period to avoid confounding effects of intra-individual seasonal variation. The mean daily maximum temperature for the month prior to trials did not differ between groups (lean 20.5±1.6°C; obese 20.6±1.6°C [mean ± SEM]) and there were no differences between the maximum temperature on the day before (lean group: placebo 23.1±2.1°C, ephedrine 19.4±1.3°C; obese group: placebo 21.4±2.1°C, ephedrine 20.2±2.4°C) or the minimum temperature on the night before (lean group: placebo 13.0±1.7°C, ephedrine 9.5±1.5°C; obese group: placebo 11.2±1.3°C, ephedrine 10.4±1.8°C) trials within or between groups.…”

Section: Methodsmentioning

confidence: 99%

Ephedrine activates brown adipose tissue in lean but not obese humans

et al. 2012

View full text Add to dashboard Cite

Aims/hypothesis Brown adipose tissue (BAT) activation increases energy consumption and may help in the treatment of obesity. Cold exposure is the main physiological stimulus for BAT thermogenesis and the sympathetic nervous system, which innervates BAT, is essential in this process. However, cold-induced BAT activation is impaired in obese humans. To explore the therapeutic potential of BAT, it is essential to determine whether pharmacological agents can activate BAT. Methods We aimed to determine whether BAT can be activated in lean and obese humans after acute administration of an orally bioavailable sympathomimetic. In a randomised, double-blinded, crossover trial, we administered 2.5 mg/kg of oral ephedrine to nine lean (BMI 22±1 kg/m 2 ) and nine obese (BMI 36±1 kg/m 2 ) young men. On a separate day, a placebo was administered to the same participants. BAT activity was assessed by measuring glucose uptake with [ 18 F]fluorodeoxyglucose and positron emission tomography-computed tomography imaging.

show abstract

Outdoor Temperature, Age, Sex, Body Mass Index, and Diabetic Status Determine the Prevalence, Mass, and Glucose-Uptake Activity of 18F-FDG-Detected BAT in Humans

Abstract: The present study identifies outdoor temperature, age, sex, BMI, and diabetes status as determinants of the prevalence, mass, and glucose-uptake activity of (18)F-FDG-detected BAT.

Cited by 496 publications

References 26 publications

Glucose uptake in human brown adipose tissue is impaired upon fasting-induced insulin resistance

Glucose uptake in human brown adipose tissue is impaired upon fasting-induced insulin resistance

Cold-induced activity of brown adipose tissue in young lean men of South-Asian and European origin

Ephedrine activates brown adipose tissue in lean but not obese humans

Contact Info

Product

Resources

About