Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics

Li, Zhi Tao; Zhang, Rui Ling; Bi, Xiaogang; Xu, Li; Fan, Min; Xie, Dan; Xian, Ying; Wang, Ying; Li, Xiao Jie; Wu, Zhong Dao; Zhang, Kou Xing

doi:10.1016/j.micpath.2015.03.009

Cited by 45 publications

(31 citation statements)

References 37 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One study supports the role of OMVs in A. baumannii pathogenesis. An A. baumannii strain that produces abundant OMVs with more virulence factors induces a stronger innate immune response and is more cytotoxic compared with those of a strain producing fewer OMVs (Li Z. T. et al, 2015). …”

Section: Acinetobacter Baumannii Virulence Factors and Pathogenesismentioning

confidence: 99%

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Lee

Park

et al. 2017

Front. Cell. Infect. Microbiol.

751

585

View full text Add to dashboard Cite

Acinetobacter baumannii is undoubtedly one of the most successful pathogens responsible for hospital-acquired nosocomial infections in the modern healthcare system. Due to the prevalence of infections and outbreaks caused by multi-drug resistant A. baumannii, few antibiotics are effective for treating infections caused by this pathogen. To overcome this problem, knowledge of the pathogenesis and antibiotic resistance mechanisms of A. baumannii is important. In this review, we summarize current studies on the virulence factors that contribute to A. baumannii pathogenesis, including porins, capsular polysaccharides, lipopolysaccharides, phospholipases, outer membrane vesicles, metal acquisition systems, and protein secretion systems. Mechanisms of antibiotic resistance of this organism, including acquirement of β-lactamases, up-regulation of multidrug efflux pumps, modification of aminoglycosides, permeability defects, and alteration of target sites, are also discussed. Lastly, novel prospective treatment options for infections caused by multi-drug resistant A. baumannii are summarized.

show abstract

Section: Acinetobacter Baumannii Virulence Factors and Pathogenesismentioning

confidence: 99%

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Lee

Park

et al. 2017

Front. Cell. Infect. Microbiol.

751

585

View full text Add to dashboard Cite

show abstract

“…Several studies on OMVs in Acinetobacter have suggested that they have many functions, including roles in horizontal gene transfer, antibiotic resistance, and virulence. A wide variety of cargo types have been identified in OMVs from different Acinetobacter strains, including virulence proteins, antibiotic resistance determinants, and DNA (96)(97)(98)(99)(100)(101)(102). An important virulence factor of A. baumannii, OmpA, has also been found to be associated with OMVs, and OMVs have been suggested to act as a mechanism for delivery for this protein to host cells (103).…”

Section: Protein Secretionmentioning

confidence: 99%

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

2016

View full text Add to dashboard Cite

The genus Acinetobacter encompasses multiple nosocomial opportunistic pathogens that are of increasing worldwide relevance because of their ability to survive exposure to various antimicrobial and sterilization agents. Among these, Acinetobacter baumannii, Acinetobacter nosocomialis, and Acinetobacter pittii are the most frequently isolated in hospitals around the world. Despite the growing incidence of multidrug-resistant Acinetobacter spp., little is known about the factors that contribute to pathogenesis. New strategies for treating and managing infections caused by multidrug-resistant Acinetobacter strains are urgently needed, and this requires a detailed understanding of the pathobiology of these organisms. In recent years, some virulence factors important for Acinetobacter colonization have started to emerge. In this review, we focus on several recently described virulence factors that act at the bacterial surface level, such as the capsule, O-linked protein glycosylation, and adhesins. Furthermore, we describe the current knowledge regarding the type II and type VI secretion systems present in these strains.

show abstract

“…Multiple studies have characterized the proteomes of A. baumannii OMVs, collectively identifying hundreds of proteins associated with or in OMVs [85, 87-89]. As mentioned above, multiple sub-types of membrane vesicles are known to be secreted by A. baumannii ; however, for the proteomic studies published, the majority of OMVs were prepared from bacteria grown to mid- to late log phase and were expected to contain prototypical OMVs (20-200nm with no inner membrane).…”

Section: Outer Membrane Vesicles and Other Secreted Proteinsmentioning

confidence: 99%

“…Naturally, a preponderance of proteins known to associate with the outer membrane were localized to OMVs, including outer membrane protein H (OmpH) and W (OmpW), outer membrane protein/protective antigen OMA87, a 33-36 kDa outer membrane protein, and multiple putative outer membrane proteins [88]. Periplasmic, inner membrane, and cytosolic proteins were also identified in or associated with OMVs, bringing in to question whether some of these components are artifacts of dying cells or actively shepherded into OMVs [88, 89]. As such, a more comprehensive understanding on the molecular mechanisms of OMV biogenesis will help to unravel whether this is an active or passive process for Acinetobacter .…”

Section: Outer Membrane Vesicles and Other Secreted Proteinsmentioning

confidence: 99%

The Secrets of Acinetobacter Secretion

Weber

Kinsella

Harding

et al. 2017

Trends in Microbiology

View full text Add to dashboard Cite

Infections caused by the bacterial pathogen Acinetobacter baumannii are a mounting concern for healthcare practitioners as widespread antibiotic resistance continues to limit therapeutic treatment options. The biological processes used by A. baumannii to cause disease are not well-defined, but recent research has indicated that secreted proteins may play a major role. A variety of mechanisms have now been shown to contribute to protein secretion by A. baumannii and other pathogenic species of Acinetobacter, including a type II and type VI secretion system, autotransporter, and outer membrane vesicles. In this review, we summarize the current knowledge of secretion systems in Acinetobacter species, and highlight their unique aspects that contribute to the pathogenicity and persistence of these emerging pathogens.

show abstract

Outer membrane vesicles isolated from two clinical Acinetobacter baumannii strains exhibit different toxicity and proteome characteristics

Cited by 45 publications

References 37 publications

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options

Pathogenic Acinetobacter: from the Cell Surface to Infinity and Beyond

The Secrets of Acinetobacter Secretion

Contact Info

Product

Resources

About