Outstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model

Mansilla, Eduardo; Spretz, Ruben; Larsen, Gustavo; Núñez, Luis; Drago, Hugo; Sturla, F.; Marín, Gustavo Horacio; Roque, Gustavo; Mártire, Karina; Aquino, Vanina Díaz; Bossi, Silvia; Gardiner, C.; Lamonega, R.; Lauzada, N.; Cordone, J.; Raimondi, J. Clemente; Tau, José María; Biasi, N.R.; Marini, J.E.; Patel, Amit N.; Ichim, Thomas E.; Riordan, Neil H; Maceira, Alberto

doi:10.1016/j.transproceed.2010.09.132

Cited by 30 publications

(17 citation statements)

References 15 publications

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“…For example, artificial skin substitutes have been shown to be more effective when stem cells are incorporated into these membranes. The quality of burn wound healing improves (Leonardi et al , 2012), reducing scar formation and re-establishing the skin appendages (Mansilla et al , 2010; Tamai et al , 2011; Huang and Burd, 2012). The treatment of a chronic static diabetic ulcer has been improved by using the patient's bone marrow (BM) mesenchymal stem cells (MSCs) in combination with autologous skin fibroblasts embedded on a biodegradable collagen membrane (Coladerm®) (Vojtassák et al , 2006).…”

Section: Introductionmentioning

confidence: 99%

Pharmacological Mobilization of Endogenous Stem Cells Significantly Promotes Skin Regeneration after Full-Thickness Excision: The Synergistic Activity of AMD3100 and Tacrolimus

Lin

Wesson

Maeda

et al. 2014

Journal of Investigative Dermatology

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Stem cell therapy has shown promise in treating a variety of pathologies including skin wounds, but practical applications remain elusive. Here we demonstrate that endogenous stem cell mobilization produced by AMD3100 and low-dose Tacrolimus is able to reduce by 25% the time of complete healing of full-thickness wounds created by surgical excision. Equally important, healing was accompanied by reduced scar and regeneration of hair follicles. Searching for mechanisms, we found that AMD3100 combined with low-dose Tacrolimus mobilized increased number of lineage-negative c-Kit+, CD34+ and CD133+ stem cells. Low-dose Tacrolimus also increased the number of SDF-1 bearing macrophages in the wounds sites amplifying the “pull” of mobilized stem cells into the wound. Lineage tracing demonstrated the critical role of CD133 stem cells in enhanced capillary and hair follicle neogenesis contributing to more rapid and perfect healing. Our findings offer a significant therapeutic approach to wound healing and tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Pharmacological Mobilization of Endogenous Stem Cells Significantly Promotes Skin Regeneration after Full-Thickness Excision: The Synergistic Activity of AMD3100 and Tacrolimus

Lin

Wesson

Maeda

et al. 2014

Journal of Investigative Dermatology

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show abstract

“…A noteworthy fact is that hair follicles and other skin appendages developed despite the severity and deepness of the burn. Even burned muscles and ribs seemed to have undergone a regenerative process by the end of the study . These results demonstrated that MSC‐integrated tissue‐engineering skin had the potential for skin regeneration.…”

Section: Application Of Msc‐based Therapy For Nonhealing Wounds—todaymentioning

confidence: 71%

Mesenchymal stem cell–based therapy for nonhealing wounds: today and tomorrow

Zhao

Hao

et al. 2015

Wound Repair Regeneration

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Although advancements have been made with traditional therapies, the treatment of chronic nonhealing wounds still remains a tough challenge. In the past two decades, mesenchymal stem cell (MSC)-based therapy has emerged as a promising therapeutic strategy for nonhealing wounds because of their characteristics including self-renewal and a multidirectional differentiation ability and their easy collection and weak immunogenicity. There is a growing body of basic scientific studies that shed light on the functional mechanism of MSCs in modulating nonhealing wounds. Furthermore, critical advances have been achieved using MSC-based therapy in preclinical animal models as well as in clinics trials. In this present review, we summarize the mechanisms of MSCs and highlight the important preclinical and clinical trials of MSC therapy for nonhealing wounds. In particular, the combination of MSCs transplantation and tissue-engineered skin is addressed as a new strategy to optimize the delivery efficiency and therapeutic potential. Additionally, the current drawbacks of MSC therapy and the potential to further optimize the use of MSCs are implied.

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“…Xue et al [42] have transplanted the MSCs onto solubilized basement membrane (Matrigel) on the burn wound. Mansilla et al [56] have used acellular dermal matrix which present a scaffold that stem cells can adhere. Local administration of the MSCs with appropriate carriers let the cells remain viable and migrate to the burn wound will increase chance of engraftment of MSCs [57].…”

Section: Delivery Methods Of Mscs In Burn Wound Studymentioning

confidence: 99%

Experimental stem cell therapies on burn wound: Do source, dose, timing and method matter?

Öztürk

Karagöz

2015

Burns

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Outstanding Survival and Regeneration Process by the Use of Intelligent Acellular Dermal Matrices and Mesenchymal Stem Cells in a Burn Pig Model

Cited by 30 publications

References 15 publications

Pharmacological Mobilization of Endogenous Stem Cells Significantly Promotes Skin Regeneration after Full-Thickness Excision: The Synergistic Activity of AMD3100 and Tacrolimus

Pharmacological Mobilization of Endogenous Stem Cells Significantly Promotes Skin Regeneration after Full-Thickness Excision: The Synergistic Activity of AMD3100 and Tacrolimus

Mesenchymal stem cell–based therapy for nonhealing wounds: today and tomorrow

Experimental stem cell therapies on burn wound: Do source, dose, timing and method matter?

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