Outstations of the golgi complex are present in the processes of cultured rat oligodendrocytes

Vries, H. de; Schrage, C; Hoekstra, K.; Kok, Jan; Haar, Marjan E. van der; Kalicharan, D.; Liem, Rsb; Copray, J.C.V.M.; Hoekstra, Dick

doi:10.1002/jnr.490360311

Cited by 24 publications

(16 citation statements)

References 15 publications

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“…In contrast, and consistent with the presence of minus-end distal microtubules, Golgi elements are present in the processes of oligodendrocytes in culture (De Vries et al, 1993), suggesting a role for cytoplasmic dynein in their translocation (Kreis, 1990). The Golgi apparatus is involved in the synthesis and processing of a number of myelin constituents (De Vries et al, 1993), including proteolipid protein (Colman et al, 1982) and myelin-associated glycoprotein (Trapp et al, 1989). Thus the presence of Golgi elements in oligodendrocyte processes allows for the synthesis of myelin components in close proximity to the site of assembly of the myelin sheath.…”

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confidence: 56%

“…This may explain the exclusion of Golgi elements from axons, because these contain uniformly plus-end distal microtubules (Baas et al, 1988). In contrast, and consistent with the presence of minus-end distal microtubules, Golgi elements are present in the processes of oligodendrocytes in culture (De Vries et al, 1993), suggesting a role for cytoplasmic dynein in their translocation (Kreis, 1990). The Golgi apparatus is involved in the synthesis and processing of a number of myelin constituents (De Vries et al, 1993), including proteolipid protein (Colman et al, 1982) and myelin-associated glycoprotein (Trapp et al, 1989).…”

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confidence: 85%

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Microtubule Organization and Stability in the Oligodendrocyte

Lunn¹,

Baas

Duncan³

1997

J. Neurosci.

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The oligodendrocyte is the glial cell responsible for the formation and maintenance of CNS myelin. Because the development of neuronal morphology is known to depend on the presence of highly organized microtubule arrays, it may be hypothesized that the properties of microtubules influence the form and function of oligodendrocytes. The goals of the present study were to define the physical attributes of microtubules in oligodendrocytes maintained in vitro. The results of electron and confocal microscopy indicate that microtubules are present throughout the cell bodies and large and small processes of oligodendrocytes and are rarely associated with discrete microtubule-organizing centers. A modified "hooking" protocol demonstrated that the polarity orientation of microtubules is uniformly plus-end distal in small oligodendrocyte processes, compared with a nonuniform, predominantly plus-end distal orientation in large processes. Oligodendrocytes were exposed to the microtubule-depolymerizing drug nocodazole to examine microtubule stability in these cells. The results suggest that oligodendrocyte microtubules can be resolved into at least three distinct microtubule populations that differ in their kinetics of depolymerization in the presence of nocodazole. These findings suggest that the properties of the oligodendrocyte microtubule array reflect the functions of the different regions of this highly specialized cell.

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confidence: 56%

mentioning

confidence: 85%

Microtubule Organization and Stability in the Oligodendrocyte

Lunn¹,

Baas

Duncan³

1997

J. Neurosci.

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“…OLGs were isolated from neonate rat spinal cord and cultured as described earlier (De Vries et al, 1993).…”

Section: Materials and Methods Isolation And Culturing Of Olgmentioning

confidence: 99%

“…The cell body is only faintly stained, which suggests that little translation occurs at this site, although it cannot entirely be excluded that minor amounts of newly synthesized protein are rapidly processed after translation, into the processes. On the other hand, PLP, which is synthesized on endoplasmic reticulum (ER) polysomes (Nussbaum and Roussel, 1983), is initially abundant in the perinuclear Golgi and, at 8 DIC, in Golgi structures near and in the primary processes, which have been identified and defined as socalled ''outstations'' of the Golgi system (De Vries et al, 1993). These structures appear to be specific for mature OLG.…”

Section: Localization Of the Major Myelin Proteins In Cultured Olgmentioning

confidence: 96%

Differential and cell development-dependent localization of myelin mRNAs in oligodendrocytes

et al. 1997

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“…OLGs were isolated from neonate rat spinal cord and cultured as described by de Vries et al (1993). In brief, OLG-enriched glia cells were isolated from the spinal cords of Wistar rats (6-to 8-d old).…”

Section: Isolation and Culture Of Neonatal Rat Olgsmentioning

confidence: 99%

An Apical-Type Trafficking Pathway Is Present in Cultured Oligodendrocytes but the Sphingolipid-enriched Myelin Membrane Is the Target of a Basolateral-Type Pathway

Vries

Schrage

Hoekstra

1998

MBoC

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Myelin sheets originate from distinct areas at the oligodendrocyte (OLG) plasma membrane and, as opposed to the latter, myelin membranes are relatively enriched in glycosphingolipids and cholesterol. The OLG plasma membrane can therefore be considered to consist of different membrane domains, as in polarized cells; the myelin sheet is reminiscent of an apical membrane domain and the OLG plasma membrane resembles the basolateral membrane. To reveal the potentially polarized membrane nature of OLG, the trafficking and sorting of two typical markers for apical and basolateral membranes, the viral proteins influenza virus-hemagglutinin (HA) and vesicular stomatitis virus-G protein (VSVG), respectively, were examined. We demonstrate that in OLG, HA and VSVG are differently sorted, which presumably occurs upon their trafficking through the Golgi. HA can be recovered in a Triton X-100-insoluble fraction, indicating an apical raft type of trafficking, whereas VSVG was only present in a Triton X-100-soluble fraction, consistent with its basolateral sorting. Hence, both an apical and a basolateral sorting mechanism appear to operate in OLG. Surprisingly, however, VSVG was found within the myelin sheets surrounding the cells, whereas HA was excluded from this domain. Therefore, despite its raft-like transport, HA does not reach a membrane that shows features typical of an apical membrane. This finding indicates either the uniqueness of the myelin membrane or the requirement of additional regulatory factors, absent in OLG, for apical delivery. These remarkable results emphasize that polarity and regulation of membrane transport in cultured OLG display features that are quite different from those in polarized cells.

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Outstations of the golgi complex are present in the processes of cultured rat oligodendrocytes

Cited by 24 publications

References 15 publications

Microtubule Organization and Stability in the Oligodendrocyte

Microtubule Organization and Stability in the Oligodendrocyte

Differential and cell development-dependent localization of myelin mRNAs in oligodendrocytes

An Apical-Type Trafficking Pathway Is Present in Cultured Oligodendrocytes but the Sphingolipid-enriched Myelin Membrane Is the Target of a Basolateral-Type Pathway

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