Oval cell proliferation in cirrhosis in rats. An experimental study

Abstract

This study demonstrates that oval cells develop and proliferate in a model of experimental liver fibrosis without pretreatment with a known hepatocytic inhibitor. However, further research is warranted in order to identify the exact molecular mechanisms involved in this process.

The complement component C3a fragment is a potential biomarker for hepatitis C virus-related hepatocellular carcinoma

“…The C3a fragment may also be elevated during hepatocyte regeneration following liver damage [38], and early diagnosis of small HCC tumors may be difficult with one marker alone. Therefore, the false-positive rates for HCC must be carefully considered [39][40][41]. Also, a combination of markers, including AFP, DCP, and the C3a fragment, in the serum should be verified to improve the diagnostic rate.…”

EGFR, HER‐2 and COX‐2 levels in colorectal cancer

“…Immunohistochemistry was performed as previously reported 15 with the following primary antibodies: anti‐EGFR (clone 31G7; Zymed, San Francisco, CA, USA; dilution 1:150), monoclonal IgG2a anti‐ERBB‐2 (F‐11; Santa Cruz, Santa Cruz, CA, USA; dilution 1:300) and polyclonal IgG anti‐COX‐2 (C‐20; Santa Cruz; dilution 1:300) and a standard streptavidin–biotin–peroxidase technique (Multilink kit; Biogenex, San Ramon, CA, USA). Diaminobenzidine (Sigma Fast DAB tablets, D‐4293; St Louis, MO, USA) was used as a chromogen.…”

“…First‐strand cDNA was synthesized from a constant quantity (1.6 μg) of total RNA as previously described 17 . The primers for EGFR , HER‐2 , COX‐2 and TATA box‐binding protein (TBP; used as a control of amplification gene) and the Taqman probes for EGFR and HER‐2 were designed as previously reported 15 and are shown in Table 1. Primers were synthesized by the Foundation for Research and Technology‐Hellas (Crete, Greece) and probes by DNA Technology A/S (Aarhus, Denmark).…”

Pantoprazole attenuates tumorigenesis via inhibition of exosomal secretion in a rat model of hepatic precancerous lesion induced by diethylnitrosamine and 2‐acetamidofluorene

“…The model of liver regeneration actually proposed is based on a complex cellular trafficking and is mediated by the release of several pleiotropic cytokines, leading to the final expansion of the oval cell department. In particular, the liver injury may trigger an immune response with the recruitment of inflammatory cells …”