Ovarian follicular fluid eicosanoid concentrations during the pre-ovulatory period in humans

Priddy, A.R.; Killick, S.R.; Elstein, Max; Morris, Jacqueline K.; Sullivan, M.; Patel, L.; Elder, M. G.

doi:10.1016/0090-6980(89)90082-8

Cited by 17 publications

(4 citation statements)

References 9 publications

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“…Subsequent observations regarding mammalian ovulation have shown increased ovarian and follicular lipoxygenase activity [16], increased LT-induced collagenolytic activity [17][18][19], inhibition of ovulation in perfused rabbit ovaries attributed to reduced LTB 4 production [20], increased ovulation number in perfused rabbit ovaries attributed to increased PG production [21], and increased vascular permeability [22]. in human follicular fluid [23] and active lipoxygenase enzymes in human granulosa cells in women undergoing in vitro fertilization [4].…”

Section: Resultsmentioning

confidence: 99%

The Lipoxygenase Inhibitor, Nordihydroguaiaretic Acid, Inhibits Ovulation and Reduces Leukotriene and Prostaglandin Levels in the Rat Ovary1

Mikuni¹,

Yoshida²,

Hellberg³

et al. 1998

Biology of Reproduction

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Eicosanoids, the active metabolites of arachidonic acid, are grouped into cyclooxygenase products (prostaglandins [PGs] and thromboxanes) and lipoxygenase products (leukotrienes [LTs] and lipoxins). Numerous studies suggest a role for the lipoxygenase system in ovulation. The aim of this study was to further characterize the effects of lipoxygenase inhibition and the interactions of the lipoxygenase and cyclooxygenase systems in the rat ovary during ovulation. The lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), was administered in vivo and in the isolated perfused rat ovary to determine its effect on ovulation rate. The in vivo study confirmed the inhibitory effect of NDGA, and in the perfusion experiments, NDGA caused a dose-dependent reduction in the ovulation rate. To further define the interaction between the lipoxygenase and cyclooxygenase systems, a second set of perfusions was performed with NDGA (10 microM) and the combination of NDGA (10 microM) plus a nonselective cyclooxygenase inhibitor, indomethacin (10 microM). NDGA significantly reduced the number of ovulations compared to that in controls. The ovulation rate for the combination of NDGA+indomethacin was also significantly lower than in controls but not different from that in the NDGA-treated group. Steroidogenesis was decreased only in the NDGA+indomethacin perfusions. Ovarian tissue PGE2 and PGF2alpha levels in the NDGA-treated ovaries were significantly suppressed compared to those in controls. Almost a complete block of PGE2 and PGF2alpha was seen in the NDGA+indomethacin group. LTB4 levels in the 10-h-perfused ovarian tissues were significantly decreased by NDGA compared to those in control tissues. Furthermore, LTB4 (3 microg added twice) completely reversed the inhibitory effect of 0.1 microM NDGA on ovulation rate and partially reversed the effect of 10 microM NDGA in the perfusion model. These results demonstrate that the products of the lipoxygenase pathway, especially LTB4, are important in the process of ovulation in this cyclically ovulating species. The interconnected lipoxygenase and cyclooxygenase pathways may optimize ovulation and facilitate steroidogenesis.

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Section: Resultsmentioning

confidence: 99%

The Lipoxygenase Inhibitor, Nordihydroguaiaretic Acid, Inhibits Ovulation and Reduces Leukotriene and Prostaglandin Levels in the Rat Ovary1

Mikuni¹,

Yoshida²,

Hellberg³

et al. 1998

Biology of Reproduction

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“…The estimated concentrations of selected PGs in HFF across all samples are shown in Table 1. PGE2 and PGF2α concentration varied widely in a small subset of patients, perhaps due to temporal regulation during oocyte maturation [6, 37] or an underlying inflammatory condition (see Discussion). In MRM mode, we did not consistently detect 6-keto -PGF1α, PGA1, PGA2, PGF3α, or PGD2 in most patient’s HFF (see Discussion).…”

Section: Resultsmentioning

confidence: 99%

Comprehensive profiling of prostaglandins in human ovarian follicular fluid using mass spectrometry

Pier

Edmonds

Wilson

et al. 2018

Prostaglandins & Other Lipid Mediators

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Prostaglandins are formed by enzymatic and non-enzymatic mechanisms. They have been detected in human ovarian follicular fluid (HFF), a medium rich in growth factors and nutrients important for oocyte growth and fertility. However, the comprehensive identification of HFF prostaglandins has not been addressed. Here we use hybrid triple quadrupole time-of-flight and triple quadrupole mass spectrometers to comprehensively analyze prostaglandins in HFF. We identified PGE1, PGE2, PGF2α, and other prostaglandins synthesized via prostaglandin-endoperoxide synthase (i.e. Cox) cascades. We also identified specific PGF2α isomers (F2-isoprostanes) and PGF3α analogs whose structures are inconsistent with Cox-dependent formation. A prospective cohort pilot study of infertility patient subtypes revealed two potential associations. F2-isoprostanes are decreased in the diminished ovarian reserve subtype and elevated PGF2α may be associated with decreased live birth. Other than PGF2α, only body mass index >25kg/m correlated with poor in vitro fertilization outcome. Our studies suggest that HFF contains prostaglandins formed from at least two mechanisms, which may correlate with distinct clinical parameters.

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“…Earlier studies have identified lipoxygenase activity (6) and measurable leukotriene levels within the preovulatory dominant follicle, in unstimulated women undergoing sterilization (17), suggesting the possible involvement of lipoxygenase products of arachidonic acid in the mechanism of ovulation. In the present study, we have demonstrated that in preovulatory FFs obtained from stimulated women undergoing IVF, there are significant concentrations of LT B4, a leukotriene which has a degranulating effect and increases vascular permeability in vivo (18).…”

Section: Discussionmentioning

confidence: 99%

Cytokines in the human ovary: Presence in follicular fluid and correlation with leukotriene B4

Bili

Tarlatzis

Daniilidis

et al. 1998

J Assist Reprod Genet

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Ovarian follicular fluid eicosanoid concentrations during the pre-ovulatory period in humans

Cited by 17 publications

References 9 publications

The Lipoxygenase Inhibitor, Nordihydroguaiaretic Acid, Inhibits Ovulation and Reduces Leukotriene and Prostaglandin Levels in the Rat Ovary1

The Lipoxygenase Inhibitor, Nordihydroguaiaretic Acid, Inhibits Ovulation and Reduces Leukotriene and Prostaglandin Levels in the Rat Ovary1

Comprehensive profiling of prostaglandins in human ovarian follicular fluid using mass spectrometry

Cytokines in the human ovary: Presence in follicular fluid and correlation with leukotriene B4

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