Ovarian Histology and Histopathology of Olive Barb, Puntius sarana Exposed to Endocrine Disrupting Chemical (17-Α Methyl Testosterone) in Laboratory Condition

Abstract

17-α methyl testosterone is a major endocrine disrupting chemical (EDCs), reported harmful effect on endocrine system of aquatic animals. To study the impact of 17-αMT on olive barb (Puntius sarana) gonad, sixty fishes were collected for in vitro experiment. Hormonal treatments were carried out 90 days with three distinct hormonal dosages (T1:40 mg/kg; T2:60 mg/kg and T3:90 mg/kg) mixed with pelleted feed and no hormone in control tank (C). Each 30 days intervals, histological analysis was performed to observe alterations in the gonad due to the effect of 17α-MT. Four gamete developmental stages of female P. sarana ovary were identified through microscopic examination named immature, previtellogenic, vitellogenic and mature stage. Mild to moderate histological abnormalities has been observed in ovary for different treatments. T1 showed apparently normal histology except some alterations like empty follicle (ef), lipid droplet (ld), vitelline envelop breakdown (veb), but pronounced alterations were detected at higher concentration T2 and T3, increasing with longer exposures for instance degenerated nucleus (dn), atretic follicle (af), thickened vitelline envelop (tve), residual oocyte (roc). This preliminary study revealed more profound responses to EDCs exposure in the olive barbs which could serve as a model bio-indicator in ecotoxicological study.

Investigation on parasitic infestation of Labeo rohita (Hamilton, 1822) in the south‐western region of Bangladesh

“…The protocol of histological slide preparation during the experiment was followed as outlined by Rahman et al. ( 2023 ), Zaman et al. ( 2023 ), and the images were taken by using Optika light microscope, Italy (B9).…”

“…The internal organ such as gill, kidney, liver, stomach and skin were taken carefully and perfectly preserved in 10% neutral buffer formalin for making histological slide preparations. The protocol of histological slide preparation during the experiment was followed as outlined by Rahman et al (2023), Zaman et al (2023), and the images were taken by using Optika light microscope, Italy (B9).…”

“…Bangladesh holds immense potential in its fisheries sector, encompassing both its inland waters and the territorial seas, which have a rich array of fish species. The country's fishery resources are characterized by a diverse spectrum, including 260 native freshwater species, 12 exotic fish species, 24 freshwater prawn species, 475 marine fish species and 36 marine shrimp species (Rahman et al, 2023;Mia et al, 2024). In our nation, fisheries resources contribute significantly to our diet, providing approximately 63% of the animal protein consumed (Ahmed, 2005).…”

Effects of Extreme-Ambient Temperatures in Silver Barb (Barbonymus gonionotus): Metabolic, Hemato-Biochemical Responses, Enzymatic Activity and Gill Histomorphology

“…Among the several species, one that stands out is the Silver barb, Barbonymus gonionotus, for its economic significance, commercial appeal, and widespread use as a food source [25]. Its great taste and high productivity have made it popular [26]. In addition, it adapts favorably to relatively low-cost and easy management techniques [27] and thus establishing it as a most suitable species for fish farmers.…”