Ovarian hormones influence immune response to Staphylococcus aureus infection

Souza, Cláudio Lima; Oliveira, Hellenn Cardoso; Júnior, Manoel Neres Santos; Silva, Mariângela de Oliveira; Coqueiro, Igor Lopes; Silva, Icaro Bonyek Santos Da; Campos, Guilherme Barreto; Silva, Robson Amaro Augusto da; Soares, Telma de Jesus; Oliveira, Márcio Vasconcelos; Timenetsky, Jorge; Marques, Lucas Miranda

doi:10.1016/j.bjid.2020.10.004

Cited by 4 publications

(6 citation statements)

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“…The incision was then sutured after the resection of both testes. For sham mice, the testes were visualised but not removed [ 24 ]. The sham surgery was performed as the control [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cells were isolated from the peritoneal lavage and cultured in RPMI 1640 medium (Gibco™) supplemented with 10% foetal bovine serum (FBS) (Sigma Aldrich) and ciprofloxacin (20 µg/mL) (Sigma Aldrich). The MPM cell suspension (2 × 10 5 /mL) was seeded in 24-well plates [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…MPMs from male sham, male OQX, and female mice were cultured with 10 μL of the inoculum of S. aureus (10 8 CFU) for 6 h at 37 °C in 5% CO 2 and 95% humidified air [ 24 ]. Part of the cells were pre-treated with 100 μL of 10 –2 M DHT for 24 h at 37 °C before inoculation with S. aureus or addition of 10 μL of sterile saline solution.…”

Section: Methodsmentioning

confidence: 99%

“…After the evaluation of viability and adequate confluence (2 × 10 5 /mL), the cells were plated in 24-well polystyrene plates and incubated for 24 h at 37 °C with 5% CO 2 before inoculation with S. aureus [ 28 ]. This procedure allowed us to obtain a homogeneous population of HPBMs, which appeared as adherent cells [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

“…HPBMs were grown using 10 μl of S. aureus inoculum (CFU 10 8 ) for 6 h in 5% CO 2 and 95% humidified air at 37 °C [ 24 ]. Additional cells in triplicate were pre-treated with 100 μl DHT (10 –2 M) for 24 h at 37 °C before stimulation with S. aureus or 10 μl of sterile saline solution.…”

Section: Methodsmentioning

confidence: 99%

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Effects of 5α-dihydrotestosterone on the modulation of monocyte/macrophage response to Staphylococcus aureus: an in vitro study

et al. 2023

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Background Staphylococcus aureus (S. aureus) is a pathogen responsible for a wide range of clinical manifestations and potentially fatal conditions. There is a paucity of information on the influence of androgens in the immune response to S. aureus infection. In this study, we evaluated the influence of the hormone 5α-dihydrotestosterone (DHT) on mouse peritoneal macrophages (MPMs) and human peripheral blood monocytes (HPBMs) induced by S. aureus. Methods An in vitro model of MPMs from BALB/c sham males, orchiectomised (OQX) males, and females was used. Cells were inoculated with 10 μL of S. aureus, phage-type 80 or sterile saline (control) for 6 h. The MPMs of OQX males and females were pre-treated with 100 μL of 10–2 M DHT for 24 h before inoculation with S. aureus. The concentration of the cytokines TNF-α, IL-1α, IL-6, IL-8, and IL-10; total nitrites (NO−2); and hydrogen peroxide (H2O2) were measured in the supernatant of MPM cultures. In addition, the toll-like receptor 2 (TLR2) and nuclear factor kappa B (NF-kB) genes that are involved in immune responses were analysed. For the in vitro model of HPBMs, nine men and nine women of childbearing age were selected and HPBMs were isolated from samples of the volunteers’ peripheral blood. In women, blood was collected during the periovulatory period. The HPBMs were inoculated with S. aureus for 6 h and the supernatant was collected for the analysis of cytokines TNF-α, IL-6, IL-12; and GM-CSF, NO−2, and H2O2. The HPBMs were then removed for the analysis of 84 genes involved in the host’s response to bacterial infections by RT-PCR array. GraphPad was used for statistical analysis with a p value < 0.05. Results Our data demonstrated that MPMs from sham males inoculated with S. aureus displayed higher concentrations of inflammatory cytokines and lower concentrations of IL-10, NO−2, and H2O2 when compared with MPMs from OQX males and females. A similar result was observed in the HPBMs of men when compared with those of women. Previous treatment with DHT in women HPBMs increased the production of pro-inflammatory cytokines and decreased the levels of IL-10, NO−2, and H2O2. The analysis of gene expression showed that DHT increased the activity of the TLR2 and NF-kB pathways in both MPMs and HPBMs. Conclusions We found that DHT acts as an inflammatory modulator in the monocyte/macrophage response induced by S. aureus and females exhibit a better immune defence response against this pathogen.

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“…The incision was then sutured after the resection of both testes. For sham mice, the testes were visualised but not removed [ 24 ]. The sham surgery was performed as the control [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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