Ovarian signalling pathways regulated by leptin during the ovulatory process

Yorio, María Paula Di; Bilbao, María Guillermina; Biagini-Majorel, Ana María; Faletti, A.

doi:10.1530/rep-13-0257

Cited by 14 publications

(3 citation statements)

References 59 publications

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“…However, direct effects of leptin, examined using superovulation models, on ovulation are also confusing with studies showing both reduced and increased number of ovulations in the rat (Almog et al 2001, Ricci et al 2006. This may be because of the number and dosage of leptin used in the treatments (Di Yorio et al 2013). Thus, an alternative approach to study leptin signalling in granulosa cells would be to investigate the importance of Lepr expression in granulosa cells.…”

Section: Introductionmentioning

confidence: 99%

Role of leptin receptors in granulosa cells during ovulation

et al. 2014

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Leptin is an important hormone influencing reproductive function. However, the mechanisms underpinning the role of leptin in the regulation of reproduction remain to be completely deciphered. In this study, our objective is to understand the mechanisms regulating the expression of leptin receptor (Lepr) and its role in ovarian granulosa cells during ovulation. First, granulosa cells were collected from superovulated mice to profile mRNA expression of Lepr isoforms (LeprA and LeprB) throughout follicular development. Expression of LeprA and LeprB was dramatically induced in the granulosa cells of ovulating follicles at 4 h after human chorionic gonadotropin (hCG) treatment. Relative abundance of both mRNA and protein of CCAAT/enhancer-binding protein b (Cebpb) increased in granulosa cells from 1 to 7 h post-hCG. Furthermore, chromatin immunoprecipitation assay confirmed the recruitment of Cebpb to Lepr promoter. Thus, hCG-induced transcription of Lepr appears to be regulated by Cebpb, which led us to hypothesise that Lepr may play a role during ovulation. To test this hypothesis, we used a recently developed pegylated superactive mouse leptin antagonist (PEG-SMLA) to inhibit Lepr signalling during ovulation. I.p. administration of PEG-SMLA (10 mg/g) to superovulated mice reduced ovulation rate by 65% compared with control treatment. Although the maturation stage of the ovulated oocytes remained unaltered, ovulation genes Ptgs2 and Has2 were downregulated in PEG-SMLA-treated mice compared with control mice. These results demonstrate that Lepr is dramatically induced in the granulosa cells of ovulating follicles and this induction of Lepr expression requires the transcription factor Cebpb. Lepr plays a critical role in the process of ovulation by regulating, at least in part, the expression of the important genes involved in the preovulatory maturation of follicles.

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Section: Introductionmentioning

confidence: 99%

Role of leptin receptors in granulosa cells during ovulation

et al. 2014

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“…Leptin is an adipocytokine produced by the visceral adipose tissue that influences gonadal function through modulating steroidogenesis in ovarian granulosa cells. Leptin has a critical role in the control of ovulation, development of a dominant follicle and oocyte maturation [12,13].…”

Section: Discussionmentioning

confidence: 99%

Serum Leptin and Adiposity Indicators in Infertile Females of Reproductive Age Group

Senghor

Shivashekar

Vinodhini

2018

Asian J Pharm Clin Res

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Objectives: This study was designed to evaluate the interrelationship of adiposity indicators such as body mass index (BMI), waist circumference (WC), hip circumference, waist–hip ratio and waist height ratio, body adiposity index (BAI) in obese and non-obese female individuals unable to conceive. And also to correlate serum leptin levels with adiposity indicators in infertile females of reproductive age group.Materials and Methods: Infertile females of reproductive age group were classified as obese and non-obese individuals based on BMI and waist–hip ratio. Serum leptin levels were estimated and correlated with adiposity indicators using Pearson’s correlation statistical analysis.Results: The mean age and duration of infertility were high in obese than the non-obese infertile females. Leptin showed significant positive correlation with WC, waist hip ratio (WHR), and waist/height ratio than the routinely done BMI which had low positive correlation. BAI had mild positive correlation with leptin.Conclusion: WC and WHR are simple convenient non-invasive tools that can differentiate the type of obesity. Adiposity indicators guide the physician to identify the infertile female individuals who require preliminary steps toward weight reduction before starting the right line of therapy to correct their hormonal imbalance.

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“…Nevertheless, some studies were unable to explain the mechanisms of higher doses of leptin supplementation (50 and 100 ng/mL) in improving the nuclear maturation rate of the oocytes. Kamalamma et al(2015) and Yorio et al (2013) state that leptin supplementation at a low dose (10 ng/mL) can activate STAT3 and ERK1 (MAPK) while at higher doses, 300-500 ng/mL, only STAT3 was activated. The results of this study are in line with some previous studies that leptin supplementation at a dose of 100 ng/mL does not increase the maturation rate of bovine oocytes (Jia et al,.…”

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confidence: 99%

Efficacy of Leptin Supplementation on Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

Riyuska

Karja

Setiadi

2019

Trop. Anim. Sci. J.

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The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. Fertilization was conducted in the oocytes supplemented with 10 ng/mL and control using 5×10 6 mL -1 spermatozoa. At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. The results showed that the percentage of oocytes reaching metaphase II (MII) stage significantly increased in the oocytes supplemented with leptin at a dose of 10 ng/mL (P<0.05) compared to those supplemented at doses of 50, 100, and 0 ng/mL (93.7±5.9% vs 78.8±4.4%; 72.0±2.6%; 82.1±9.9%). However, fertilization rate of the oocytes supplemented with leptin at a dose of 10 ng/mL and control were similar (72.1±5.5% vs 79.2±7.0%). The data indicated that leptin could improve maturation rate in lower concentration. However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate.

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Ovarian signalling pathways regulated by leptin during the ovulatory process

Cited by 14 publications

References 59 publications

Role of leptin receptors in granulosa cells during ovulation

Role of leptin receptors in granulosa cells during ovulation

Serum Leptin and Adiposity Indicators in Infertile Females of Reproductive Age Group

Efficacy of Leptin Supplementation on Nuclear Maturation and Fertilization Rate of Sheep Oocytes In Vitro

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