Ovarian stimulation protocols based on follicle-stimulating hormone glycosylation pattern: impact on oocyte quality and clinical outcome

Selman, Helmy; Pacchiarotti, Arianna; El-Danasouri, Imam

doi:10.1016/j.fertnstert.2009.10.005

Cited by 26 publications

(23 citation statements)

References 49 publications

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“…Our results showed that this strategy led to a trend towards a higher, even if not significant pregnancy rate, as mentioned earlier [26][27][28]. The use of hFSH yields the same results as the use of recombinant FSH, in agreement with several other authors [26,27].…”

Section: Discussionsupporting

confidence: 92%

Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Gürgan

Montjean²,

Ménézo

2014

J Assist Reprod Genet

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FSH is a key hormone in the regulation of follicular development. Together with the EGF network, these molecules mediate oocyte maturation and competence in preparation for the action of LH. FSH isoforms regulate distinct biological pathways and have specific effects on granulosa cell function and maturation of the ovarian follicle. Their dynamic interactions occur during the follicular cycle; shortliving forms are predominant in the pre-ovulatory phase, whereas long-acting molecules characterize the lutealfollicular transition. Recombinant FSH (rFSH) molecules have a reduced number of isoforms and are less acidic, with a shorter half-life. We have investigated sequential stimulation, comparing hFSH + rFSH, vs. rFSH alone and hFSH alone for the entire stimulation phase. Sequential stimulation leads to an E2 per MII oocyte ratio that is much lower than is seen during treatment with the two drugs individually. Although there is a positive tendency in favor of the sequential treatment, there was no significant difference in pregnancy rates, even taking frozen embryos into consideration. The cumulus cell transcriptome varies considerably between the treatments, although with no clear significance. When comparing pregnant vs. non-pregnant patients, in general a decrease in mRNA expression can be observed in the pregnant patients, especially in expression of folic acid receptor 1 and ovostatin 2. This indicates that material has been transferred from CC to the oocyte. However, a common observation in the literature is that variations in the transcriptome of the cumulus cells are highly dependent upon the patient genotype; the potential for applying this strategy as a basis for selecting embryos is, at the very least, questionable.

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Section: Discussionsupporting

confidence: 92%

Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Gürgan

Montjean²,

Ménézo

2014

J Assist Reprod Genet

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“…The ratio of FSH 21 to FSH 24 decreases with increasing age such that in postmenopausal women hFSH 24 is the dominant glycoform. Although the reasons for the switch from hypo-glycosylated hFSH to fully-glycosylated hFSH are not understood at present, a study by Selman (16) reported that FSH preparations with different glycosylation patterns differentially impacted clinical outcomes in patients being treated for infertility. Moreover, the profound increase in circulating levels of hFSH at menopause (15) highlights the importance of understanding how FSH glycosylation variants alter ovarian function.…”

mentioning

confidence: 99%

Hypoglycosylated hFSH Has Greater Bioactivity Than Fully Glycosylated Recombinant hFSH in Human Granulosa Cells

Jiang

Hou

Wang

et al. 2015

The Journal of Clinical Endocrinology & Metabolism

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“…Moreover they received combined protocol [20][21][22] starting with 225 IU of acidic HMG (Meropur; Ferring Italy) for the first 6 days starting from day 2 of the cycle and followed by 225 IU of less-acidic recombinant FSH (Puregon, MSD, Rome, Italy) until hCG administration.…”

Section: Treatment and Protocols Of Stimulationmentioning

confidence: 99%

Antioxidative Capacity Of Melatonin In Follicular Fluid Of Aged IVF Patients: Beneficial Effects On Oocytes And Embryo

Pacchiarotti¹,

Pacchiarotti²,

Valeri³

et al. 2015

JGNB

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The aim of the present study was to evaluate the role of melatonin supplementation on the main IVF outcomes in aged patients underwent IVF. 358 infertile women aged over 40 underwent a shortdown-regulation protocol and were randomized into two groups: 178 patients who received 5mg melatonin (group A) and 180 patients who did not received melatonin (group B). Oxidative stress values, mature oocytes, embryo quality, pregnancy rates and implantation rates, intrafollicular concentration of melatonin and progesterone were measured.There were significant statistical differences comparing group A with group B in terms of mature oocytes (48.2% vs 35.0%), oxidative stress (CARR U) (190 ± 41 vs 388 ± 64), antioxidative capacity (AOCs) (1,76±0,4 vs 0,89 ± 0,2), progesterone concentration in follicular fluid (10,4±1.1 ml vs 4,3±0,8 ml) and grade I embryos (45,7% vs 30,4%, p=0.0045).Melatonin intrafollicular concentrations were significantly increased after melatonin treatment (213±35 pg/ml versus 69 ± 23 pg/ml). In conclusion melatonin supplementations during ovarian stimulationin aged patients improve oocyte and embryo quality, increasing progesterone production and scavenging free radicals. Furthermorewe demonstrated that exogenous administrated melatonin is able to accumulate efficiently in the follicular fluid.

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Ovarian stimulation protocols based on follicle-stimulating hormone glycosylation pattern: impact on oocyte quality and clinical outcome

Cited by 26 publications

References 49 publications

Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Hypoglycosylated hFSH Has Greater Bioactivity Than Fully Glycosylated Recombinant hFSH in Human Granulosa Cells

Antioxidative Capacity Of Melatonin In Follicular Fluid Of Aged IVF Patients: Beneficial Effects On Oocytes And Embryo

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