Ovarian Transcriptome Analysis of Vitellogenic and Non-Vitellogenic Female Banana Shrimp (Fenneropenaeus merguiensis)

Saetan, Uraipan; Sangket, Unitsa; Deachamag, Panchalika; Chotigeat, Wilaiwan

doi:10.1371/journal.pone.0164724

Cited by 21 publications

(6 citation statements)

References 39 publications

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“…In crustacean species, a neuroparsin-like peptide (MeNPLP) which is produced by the hepatopancreas and has been reported to induce VG expression in M. ensis ( 65 ). Transcriptomic analysis of Fenneropenaeus merguiensis (the banana shrimp) ovary shows that the expression of a neuroparsin precursor is higher in the vitellogenic stage compared to that in the non-vitellogenic stage ( 70 ). In the present study, we showed that Maj-NPLP has a stimulatory effect on VG synthesis in the ovary ( Figure 6A ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of the Kuruma Prawn Marsupenaeus japonicus Reveals Possible Peripheral Regulation of the Ovary

et al. 2020

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Crustacean reproduction has been hypothesized to be under complex endocrinological regulation by peptide hormones. To further improve our understanding of the mechanisms underlying this complex regulation, knowledge is needed regarding the hormones not only of the central nervous system (CNS) such as the X-organ/sinus gland (XOSG), brain, and thoracic ganglia, but also the peripheral gonadal tissues. For example, in vertebrates, some gonadal peptide hormones including activin, inhibin, follistatin, and relaxin are known to be involved in the reproductive physiology. Therefore, it is highly likely that some peptide factors from the ovary are serving as the signals among peripheral tissues and central nervous tissues in crustaceans. In this work, we sought to find gonadal peptide hormones and peptide hormone receptors by analyzing the transcriptome of the ovary of the kuruma prawn Marsupenaeus japonicus. The generated ovarian transcriptome data led to the identification of five possible peptide hormones, including bursicon-α and-β, the crustacean hyperglycemic hormone (CHH)-like peptide, insulin-like peptide (ILP), and neuroparsin-like peptide (NPLP). Dominant gene expressions for the bursicons were observed in the thoracic ganglia and the ovary, in the CNS for the CHH-like peptide, in the heart for NPLP, and in the ovary for ILP. Since the gene expressions of CHH-like peptide and NPLP were affected by a CHH (Penaeus japonicus sinus gland peptide-I) from XOSG, we produced recombinant peptides for CHH-like peptide and NPLP using Escherichia coli expression system to examine their possible peripheral regulation. As a result, we found that the recombinant NPLP increased vitellogenin gene expression in incubated ovarian tissue fragments. Moreover, contigs encoding putative receptors for insulin-like androgenic gland factor, insulin, neuroparsin, and neuropeptide Y/F, as well as several contigs encoding orphan G-protein coupled receptors and receptor-type guanylyl cyclases were also identified in the ovarian transcriptome. These results suggest that reproductive physiology in crustaceans is regulated by various gonadal peptide hormones, akin to vertebrates.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of the Kuruma Prawn Marsupenaeus japonicus Reveals Possible Peripheral Regulation of the Ovary

et al. 2020

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“…Short-read sequencing (Illumina or 454) has been used to produce transcriptomes of some shrimp species, including L. vannamei 17 , 18 , 33 – 40 , Fenneropenaeus merguiensis 41 , 42 , Macrobrachium rosenbergii 43 , Triops newberryi 44 , T. longicaudatus 45 , Pandalus latirostris 46 , Fenneropenaeus chinensis 47 , Palaemon serratus 48 , and Penaeus monodon 49 . The average lengths of transcripts obtained in these studies were ~306–1,027 bp.…”

Section: Discussionmentioning

confidence: 99%

Single-molecule long-read sequencing facilitates shrimp transcriptome research

Zeng

Chen

Peng

et al. 2018

Sci Rep

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Although shrimp are of great economic importance, few full-length shrimp transcriptomes are available. Here, we used Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology to generate transcripts from the Pacific white shrimp (Litopenaeus vannamei). We obtained 322,600 full-length non-chimeric reads, from which we generated 51,367 high-quality unique full-length transcripts. We corrected errors in the SMRT sequences by comparison with Illumina-produced short reads. We successfully annotated 81.72% of all unique SMRT transcripts against the NCBI non-redundant database, 58.63% against Swiss-Prot, 45.38% against Gene Ontology, 32.57% against Clusters of Orthologous Groups of proteins (COG), and 47.83% against Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Across all transcripts, we identified 3,958 long non-coding RNAs (lncRNAs) and 80,650 simple sequence repeats (SSRs). Our study provides a rich set of full-length cDNA sequences for L. vannamei, which will greatly facilitate shrimp transcriptome research.

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“…Fm‐15 is a microsatellite primer (Fm15_ATC7, GenBank: OM640622) of F. merguiensis , and was designed based on the results of transcriptome data analysis (Saetan et al, 2016). The mitochondrial cytochrome oxidase subunit I gene (COI) from F. merguiensis (GenBank: OM257430) was designed based on highly conserved regions of COI genes (Folmer et al, 1994).…”

Section: Methodsmentioning

confidence: 99%

Molecular markers for differentiation of Fenneropenaeus merguiensis among Penaeus species

Chimnual

Saetan

Choomee

et al. 2022

Aquaculture Research

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Shrimp farming is a substantial income source in many countries. Farming is well established for some shrimp species, such as Penaeus vannamei (Pacific white shrimp), Macrobrachium rosenbergii (Freshwater shrimp) and Penaeus monodon (Black tiger shrimp).Despite Fenneropenaeus merguiensis (Banana shrimp) being wellknown to consumers, most of this shrimp production is from capture fisheries. This situation has led to overfishing of F. merguiensis, eventually damaging the 'species' genetic diversity and even threatening the extinction of this shrimp.Recently, transplantation technology has been applied successfully to several fish, including Nile tilapia (Oreochromis niloticus), rainbow trout (Oncorhynchus mykiss) and tiger puffer (Takifugu rubripes) (Farlora et al., 2014;Okutsu et al., 2007;Yoshikawa et al., 2018). This technique allows the transfer of the spermatogonia of a donor fish that exhibits desirable characteristics into a female recipient. This technology could be helpful in the genetic diversity conservation of F. merguiensis. However, applying this technology, that is, transferring spermatogonia from F. merguiensis to recipients of the same species or other shrimp species that could be good breeding partners, requires the availability of molecular markers that can be used to follow the genetic contributions of the donor in the recipient. VASA (Ddx4) is one of the markers used to detect germ cells in animals. The VASA gene is a member of the DEAD-box family (Asp-Glu-Ala-Asp) of ATP-dependent RNA helicases and is

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Ovarian Transcriptome Analysis of Vitellogenic and Non-Vitellogenic Female Banana Shrimp (Fenneropenaeus merguiensis)

Cited by 21 publications

References 39 publications

Transcriptomic Analysis of the Kuruma Prawn Marsupenaeus japonicus Reveals Possible Peripheral Regulation of the Ovary

Transcriptomic Analysis of the Kuruma Prawn Marsupenaeus japonicus Reveals Possible Peripheral Regulation of the Ovary

Single-molecule long-read sequencing facilitates shrimp transcriptome research

Molecular markers for differentiation of Fenneropenaeus merguiensis among Penaeus species

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