Over-expression of 3-hydroxy-3- methylglutaryl-coenzyme A reductase 1 (hmgr1) gene under super-promoter for enhanced latex biosynthesis in rubber tree (Hevea brasiliensis Muell. Arg.)

Rout, Jayashree; Nazeem, P.; Rekha, K.; Sreelatha, S.; Thulaseedharan, A.; Krishnakumar, R.; Kala, R. G.; Vineetha, M.; Leda, P.; Jinu, Udayabhanu; Venkatachalam, P.

doi:10.1016/j.plaphy.2018.04.011

Cited by 22 publications

(12 citation statements)

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“…Although a large number of genomic data and transcriptome data of rubber tree can be obtained [9][10][11], but few functional genes have been proved in rubber tree [12][13][14][15]. Functional gene analysis is still a major challenge lacking e cient and reliable genetic transformation system [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…The physiological functions of rubber molecule and latex remain intrigue [7,8]. To date, rubber biosynthesis pathway has been well characterized [8], the whole-genome data and transcriptome database became available in rubber tree [9][10][11], but only a few NR biosynthesis-related genes functions are evaluated by transgenic rubber tree due to low genetic transformation e ciency [12][13][14][15]. The development of an e cient and reliable rubber tree transformation system is still challenging [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Wang

Zhu

et al. 2020

Preprint

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BackgroundSince it is very difficult to obtain gene knockouts in rubber tree (Hevea Brasiliensis) due to low genetic transformation efficiency. Virus-induced gene silencing (VIGS) is a powerful gene silencing tool that has been intensively applied in plant. Up to now, the application of VIGS in rubber tree has not yet been reported.ResultsHevea brasiliensis phytoene desaturase (HbPDS) was identified in H. brasiliensis genome. The prediction of small interfering RNAs (siRNAs) from HbPDS and the silencing gene fragment (SGF) were predicted and a length of 409 bp SGF was chosen to be tested. We show that the tobacco rattle virus (TRV) -VIGS is able to induce effective HbPDS silencing in rubber tree. The TRV-VIGS system has the potential for functional gene studies in rubber tree.ConclusionsThis is the first time to report VIGS in rubber tree. The present TRV-VIGS method could be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree. The applied TRV-VIGS method will achieve deeper underground into the natural rubber biosynthesis and regulation in this important rubber-producing plant.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Wang

Zhu

et al. 2020

Preprint

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“…It is well-accepted that HMGR is a key enzyme of the MVA pathway, which catalyzes the conversion of 3-hydroxy-methylglutaryl-CoA to mevalonate (Cao et al, 2010). Previous studies have suggested that over-expression of HMGR genes significantly increased the accumulation of terpenoids (Jayashree et al, 2018). FPP and GGPP are the precursors for the biosynthesis of sesquiterpenes and diterpenes, respectively.…”

Section: Discussionmentioning

confidence: 99%

Identification, expression, and phylogenetic analyses of terpenoid biosynthesis-related genes in secondary xylem of loblolly pine (Pinus taeda L.) based on transcriptome analyses

Mao

Hao

et al. 2019

PeerJ

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Loblolly pine (Pinus taeda L.) is one of the most important species for oleoresin (a mixture of terpenoids) in South China. The high oleoresin content of loblolly pine is associated with resistance to bark beetles and other economic benefits. In this study, we conducted transcriptome analyses of loblolly pine secondary xylem to gain insight into the genes involved in terpenoid biosynthesis. A total of 372 unigenes were identified as being critical for oleoresin production, including genes for ATP-binding cassette (ABC) transporters, the cytochrome P450 (CYP) protein family, and terpenoid backbone biosynthesis enzymes. Six key genes involved in terpenoid biosynthetic pathways were selected for multiple sequence alignment, conserved motif prediction, and phylogenetic and expression profile analyses. The protein sequences of all six genes exhibited a higher degree of sequence conservation, and upstream genes were relatively more conserved than downstream genes in terpenoid biosynthetic pathways. The N-terminal regions of these sequences were less conserved than the C-terminal ends, as the N-terminals were quite diverse in both length and composition. The phylogenetic analyses revealed that most genes originated from gene duplication after species divergence, and partial genes exhibited incomplete lineage sorting. In addition, the expression profile analyses showed that all six genes exhibited high expression levels during the high-oleoresin-yielding phase.

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“…HMGR is usually divided into HMGR1, HMGR2 and HMGR3 [42]. The overexpression of HMGR1 and HMGR3 can also have a positive effect on the synthesis of secondary metabolites [42][43]. The expression level of HMGR1 gene (TRINITY_DN83281_c2_g2) enriched in all the twigs was not signi cantly changed, but clearly decreased in the leaves by the compost treatment.…”

Section: Identi Tion Of Degs In Kegg Pathwaysmentioning

confidence: 99%

Transcriptomic Analysis of Fertilization Effects on the Biosynthesis of Sesquiterpenes in Essential Oils from Phoebe Bournei Twigs and Leaves

Liu¹,

He²,

Xu³

et al. 2021

Preprint

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Background: Phoebe bournei is a potential medicinal plant whose essential oil (EO) from leaves has potential inhibitory activities against some bacterium, tumor, and has a certain potential for hypoglycemic activity. Fertilization is a common and effective method to increase plant biomass, which can increase the raw material of essential oil, but has a certain impact on the composition and biological activity of plant essential oil. Results: The main components are sesquiterpenes in the essential oils from leaves and twigs. The yield of the essential oils and the content of their main components can be modulated by compost and compound fertilizer, to different degrees, and minor differences were registered among the categories of the components in essential oils. However, changes were strongly mirrored in some main components of essential oils. The content of the primary (+) - calarene in the leaf EO were strongly increased by compost, but the opposite happened by compound fertilizer. On the contrary, the effect of compound fertilizer was more significant on the main components of twig essential oil than compost. The transcriptome sequencing results of P. bournei showed that the total number of DEGs in twigs and leaves treated with compost were significantly more than that with compound fertilizer. No change was found in the expression of genes regulating principal components. However, the expression of several key genes regulating the upstream substrates for the synthesis of the sesquiterpenes was significantly changed: the expression of two key speed limiting enzymes genes (DXS and HMGR) and two important branch-point enzyme genes (FPPS and GGPPS) was significantly down regulated, while the expression of gene (HMGS) was significantly up-regulated.Conclusion: The expression levels of genes (DXS2, HMGR, FPPS and GGPPS) were significantly down regulated in leaves treated with compost, resulting in the changes of the yield and main components of the leaf essential oil. The effect of compost was more significant on the synthesis of the essential oil from P. bournei leaves than that of compound fertilizer.

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Over-expression of 3-hydroxy-3- methylglutaryl-coenzyme A reductase 1 (hmgr1) gene under super-promoter for enhanced latex biosynthesis in rubber tree (Hevea brasiliensis Muell. Arg.)

Cited by 22 publications

References 33 publications

Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Identification, expression, and phylogenetic analyses of terpenoid biosynthesis-related genes in secondary xylem of loblolly pine (Pinus taeda L.) based on transcriptome analyses

Transcriptomic Analysis of Fertilization Effects on the Biosynthesis of Sesquiterpenes in Essential Oils from Phoebe Bournei Twigs and Leaves

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