Over-Expression of a Tobacco Nitrate Reductase Gene in Wheat (Triticum aestivum L.) Increases Seed Protein Content and Weight without Augmenting Nitrogen Supplying

Zhao, Xiaoqiang; Nie, Xuan-Li; Xiao, Xia

doi:10.1371/journal.pone.0074678

Cited by 27 publications

(17 citation statements)

References 41 publications

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“…Recently, it was reported that overexpression of a tobacco NR gene in wheat increased the seed protein content, without the need for increased N fertilization (Zhao et al. ). Such an interesting finding could rekindle the possibility of using NR as a breeding target to improve wheat NUE, yield and grain quality.…”

Section: Breeding For Nuementioning

confidence: 99%

Breeding for increased nitrogen‐use efficiency: a review for wheat (T. aestivum L.)

et al. 2016

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Nitrogen fertilizer is the most used nutrient source in modern agriculture and represents significant environmental and production costs. In the meantime, the demand for grain increases and production per area has to increase as new cultivated areas are scarce. In this context, breeding for an efficient use of nitrogen became a major objective. In wheat, nitrogen is required to maintain a photosynthetically active canopy ensuring grain yield and to produce grain storage proteins that are generally needed to maintain a high end-use quality. This review presents current knowledge of physiological, metabolic and genetic factors influencing nitrogen uptake and utilization in the context of different nitrogen management systems. This includes the role of root system and its interactions with microorganisms, nitrate assimilation and its relationship with photosynthesis as postanthesis remobilization and nitrogen partitioning. Regarding nitrogen-use efficiency complexity, several physiological avenues for increasing it were discussed and their phenotyping methods were reviewed. Phenotypic and molecular breeding strategies were also reviewed and discussed regarding nitrogen regimes and genetic diversity

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Section: Breeding For Nuementioning

confidence: 99%

Breeding for increased nitrogen‐use efficiency: a review for wheat (T. aestivum L.)

et al. 2016

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“…Similarly, low N supply from sodium nitrate and potassium nitrate resulted in a significant increase in the nitrate/nitrite mRNA production in wheat (Tritium aestivum), whereas high concentrations suppressed the expression of NR (Kavoosi et al, 2014). Furthermore, overexpression of a tobacco (Nicotiana tabacum) NR gene in wheat improved NUE and increased seed protein content and weight without augmenting N supply (Zhao et al, 2013). The results suggested a positive role of NR in N metabolism.…”

mentioning

confidence: 92%

Leaf and Root Growth, Carbon and Nitrogen Contents, and Gene Expression of Perennial Ryegrass to Different Nitrogen Supplies

Jiang

Nie

et al. 2016

J. Amer. Soc. Hort. Sci.

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Nitrogen greatly impacts plant growth and development. The objective of this study was to characterize growth, nitrogen use, and gene expression of perennial ryegrass (Lolium perenne) in response to increasing nitrogen supplies. Perennial ryegrass (cv. Inspire) was grown in sand culture and irrigated with a half-Hoagland solution amended with 0, 0.5, 1.0, 2.5, 5.0, and 7.5 mm nitrogen. Leaf tissues were harvested at 10 days (first cutting) and 20 days (second cutting) and roots were harvested at 20 days. The relatively higher N supply (2.0–7.5 mm) resulted in a larger amount of leaf fresh and dry weight but lower root fresh and dry weight, especially for the second cutting. Root:leaf ratio was higher under low N, but lower under the high N treatment. Leaf N content was relatively higher under 2.5, 5, and 7.5 mm N than under the other three treatments, while 2.5 mm N exhibited relatively higher leaf carbon content for both cuttings. Leaf C:N ratio and leaf nitrogen use efficiency (LNUE) decreased with increasing N supplies for the first cutting but were higher under low N (0–1.0 mm) for both cuttings. Leaf C:N ratio and LNUE did not differ among low N and LNUE also remained unchanged among high N for the second cutting. Root N content increased, but the root C:N ratio and root N use efficiency (RNUE) decreased with increasing N supplies, especially under 2.5, 5.0, and 7.5 mm N. Low (0.5 mm), moderate (2.5 mm), and high (7.5 mm) N were chosen to examine the expression level of NR encoding nitrate reductase and GS1b encoding glutamine synthetase. Treatment of 0.5 mm N had higher expression levels of leaf NR than other two treatments for both cuttings and a higher level of leaf GS for the second cutting. Expression of NR in the roots did not vary among treatments but the expression of GS increased under 2.5 and 7.5 mm, compared with the 0.5 mm N. Differential leaf and root growth and physiological responses to low N (0 to 1 mm) and to moderate to high N (2.5 to 7.5 mm) could be used for examining the natural variation of N use in diverse perennial ryegrass populations.

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“…The NR substrate NO 3 − has also been shown to regulate flowering induction (Marín et al, 2011), crop growth and yield increments (Lawlor, 2002;Krapp et al, 2014). Moreover, the over-expression of tobacco NR increases seed protein content and weight in wheat without increasing the N supply (Zhao et al, 2013). We report here that the transgenic tobacco line that express more OtNOS transcript levels presented a positive correlation with protein content, NR expression and seed production.…”

Section: Discussionmentioning

confidence: 59%

Nitrogen Depletion Blocks Growth Stimulation Driven by the Expression of Nitric Oxide Synthase in Tobacco

et al. 2020

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Nitric oxide (NO) is a messenger molecule widespread studied in plant physiology. Latter evidence supports the lack of a NO-producing system involving a NO synthase (NOS) activity in higher plants. However, a NOS gene from the unicellular marine alga Ostreococcus tauri (OtNOS) was characterized in recent years. OtNOS is a genuine NOS, with similar spectroscopic fingerprints to mammalian NOSs and high NO producing capacity. We are interested in investigating whether OtNOS activity alters nitrogen metabolism and nitrogen availability, thus improving growth promotion conditions in tobacco. Tobacco plants were transformed with OtNOS under the constitutive CaMV 35S promoter. Transgenic tobacco plants expressing OtNOS accumulated higher NO levels compared to siblings transformed with the empty vector, and displayed accelerated growth in different media containing sufficient nitrogen availability. Under conditions of nitrogen scarcity, the growth promoting effect of the OtNOS expression is diluted in terms of total leaf area, protein content and seed production. It is proposed that OtNOS might possess a plant growth promoting effect through facilitating N remobilization and nitrate assimilation with potential to improve crop plants performance.

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Over-Expression of a Tobacco Nitrate Reductase Gene in Wheat (Triticum aestivum L.) Increases Seed Protein Content and Weight without Augmenting Nitrogen Supplying

Cited by 27 publications

References 41 publications

Breeding for increased nitrogen‐use efficiency: a review for wheat (T. aestivum L.)

Breeding for increased nitrogen‐use efficiency: a review for wheat (T. aestivum L.)

Leaf and Root Growth, Carbon and Nitrogen Contents, and Gene Expression of Perennial Ryegrass to Different Nitrogen Supplies

Nitrogen Depletion Blocks Growth Stimulation Driven by the Expression of Nitric Oxide Synthase in Tobacco

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