Over-Expression of an R2R3 MYB Gene, MdMYB108L, Enhances Tolerance to Salt Stress in Transgenic Plants

Du, Bingyang; Liu, Heng; Dong, Kuntian; Wang, Yong; Zhang, Yuanhu

doi:10.3390/ijms23169428

Cited by 17 publications

(9 citation statements)

References 63 publications

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Section: Discussionmentioning

confidence: 99%

“…Many reports have described that MYB TFs are involved in response to abiotic stress in different plants (Ambawat et al, 2013;Jung et al, 2008;Zhang et al, 2011). In several works, different MYB TFs were up-regulated by stress conditions and different approaches have demonstrated the role of MYB genes in response to abiotic stress (An et al, 2018;Du et al, 2022;Wang et al, 2021;Yang et al, 2012;Zhou et al, 2022) have reported chlorophyll-deficient soybean plants with a concomitant reduction in isoflavone levels (Mikuriya et al, 2017;Shiroshita et al, 2021). Also interestingly, a recent work in cucumber has reported that Csmyb36 mutants have also lower chlorophyll content concluding that CsMYB36 may regulate the yellow-green coloration in cucumber by interacting and/or changing the expression levels of a pigment protein gene and other genes (Hao et al, 2018).…”

Section: Ljmyb36 Mutants Show Higher Sensitivity To Salt Light and Th...mentioning

confidence: 99%

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Mutation of MYB36 affects isoflavonoid metabolism, growth, and stress responses in Lotus japonicus

Monje‐Rueda,

Pal'ove‐Balang,

Trush

et al. 2023

Physiologia Plantarum

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Isoflavonoids are mostly produced by legumes although little is known about why and how legumes are able to regulate the biosynthesis of these particular compounds. Understanding the role of potential regulatory genes of the isoflavonoid biosynthetic pathway constitutes an important topic of research. The LORE1 mutation of the gene encoding the transcription factor MYB36 allowed the identification of this gene as a regulator of isoflavonoid biosynthesis in Lotus japonicus plants. The levels of several isoflavonoid compounds were considerably lower in two lines of Ljmyb36 mutant plants compared to the WT. In addition, we found that Ljmyb36 mutant plants were significantly smaller and showed a substantial decrease in the chlorophyll levels under normal growth conditions. The analysis of plants subjected to different types of abiotic stress conditions further revealed that mutant plants presented a higher sensitivity than WT plants, indicating that the MYB36 transcription factor is also involved in the stress response in L. japonicus plants.

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Section: Discussionmentioning

confidence: 99%

Section: Ljmyb36 Mutants Show Higher Sensitivity To Salt Light and Th...mentioning

confidence: 99%

Mutation of MYB36 affects isoflavonoid metabolism, growth, and stress responses in Lotus japonicus

Monje‐Rueda,

Pal'ove‐Balang,

Trush

et al. 2023

Physiologia Plantarum

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“…The soluble sugar content (mg/g) and proline content (µg/g) were measured using the anthrone method [26] and the ninhydrin method [27], respectively. To measure the soluble sugar content, 0.5 g fresh leaves tissue was boil water bathed for 20 min with 15 mL distilled water.…”

Section: Biochemical Analysesmentioning

confidence: 99%

“…The peroxidase (POD) activity (U/g) was measured with the guaiacol method [27]. To obtain the enzyme solution, 1 g of fresh leaves tissue was ground with 5 mL 0.1 mol•L −1 phosphate buffer solution (pH = 6).…”

Section: Biochemical Analysesmentioning

confidence: 99%

Physio-Biochemical Responses of Three Aquilegia Species Seedlings to Salt Stress

et al. 2022

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Road deicing salts are widely used during winter in northern China, which makes it essential to choose proper salt-tolerant plant species in urban landscapes. Columbine (Aquilegia) is a herb with high ornamental and commercial values. This study evaluated three Aquilegia species (A. oxysepala, A. parviflora, and A. viridiflora) for salt tolerance by monitoring their germination state under different electrical conductivity (EC) of 0.1 dS·m−1 (distilled water), 1.0 dS·m−1, 2.0 dS·m−1, 3.0 dS·m−1, 4.0 dS·m−1, 5.0 dS·m−1, 6.0 dS·m−1, physio-biochemical responses to different EC of 0.3 dS·m−1 (tap water), 5.0 dS·m−1, and 10.0 dS·m−1. The germination and growth parameters, visual scores, dry weight, leaf stomatal conductance, photosynthetic rate, and chlorophyll contents of three species decreased under salt stress, which was opposite to the changes of electrolyte leakage, malondialdehyde, proline, and soluble sugar contents. Superoxide dismutase and peroxidase activity trend differently among species. These results showed that the germination threshold of three species was 6.0 dS·m−1. A. oxysepala was the most salt-tolerant species, with a tolerance threshold of soil conductivity in 2.83 dS·m−1, followed by A. viridiflora and A. parviflora. Therefore, A. oxysepala is suitable for planting as a ground cover in urban areas where deicing salt is applied.

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“…The 2R-MYB genes, named R2R3-MYB, were usually the largest MYB gene subfamily in green plants ( Martin and Paz-Ares, 1997 ). They play essential roles in plant lifetime, including (1) primary and secondary metabolites, such as phenylpropanoid metabolism ( Liu et al., 2015 ; Tang et al., 2021 ), flavonoid biosynthesis ( Czemmel et al., 2012 ; Lin et al., 2021 ; Zhao et al., 2022b ), anthocyanin biosynthesis ( Naing and Kim, 2018 ; Upadhyaya et al., 2021 ; Wang et al., 2022c ), chlorogenic acid biosynthesis ( Tang et al., 2021 ), indolic and aliphatic glucosinolate biosynthesis ( Gigolashvili et al., 2009 ), and apigenin biosynthesis ( Wang et al., 2022b ); (2) cell fate and identity, for instance, epidermis and root formation ( Du et al., 2009 ); (3) developmental processes, like embryogenesis ( Wang et al., 2009 ), pollen development ( Liu et al., 2021 ), male sterility ( Yu et al., 2021 ), and plant trichome development ( Shangguan et al., 2021 ); and (4) responses to biotic and abiotic stresses, including diseases ( Shen et al., 2017 ; Gu et al., 2020 ; Hawku et al., 2022 ), high salinity ( Wang et al., 2021a ; Du et al., 2022 ), cold ( Dong et al., 2021 ), heat ( Wu et al., 2021b ), and drought ( Lv et al., 2021 ; Wu et al., 2021a ).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the MYB gene family in seven Ipomoea species

Wang

et al. 2023

Front. Plant Sci.

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The MYB transcription factors regulate plant growth, development, and defense responses. However, information about the MYB gene family in Ipomoea species is rare. Herein, we performed a comprehensive genome-wide comparative analysis of this gene family among seven Ipomoea species, sweet potato (I. batatas), I. trifida, I. triloba, I. nil, I. purpurea, I. cairica, and I. aquatic, and identified 296, 430, 411, 291, 226, 281, and 277 MYB genes, respectively. The identified MYB genes were classified into five types: 1R-MYB (MYB-related), 2R-MYB (R2R3-MYB), 3R-MYB (R1R2R3-MYB), 4R-MYB, and 5R-MYB, and the MYB-related or R2R3-MYB type was the most abundant MYB genes in the seven species. The Ipomoea MYB genes were classed into distinct subgroups based on the phylogenetic topology and the classification of the MYB superfamily in Arabidopsis. Analysis of gene structure and protein motifs revealed that members within the same phylogenetic group presented similar exon/intron and motif organization. The identified MYB genes were unevenly mapped on the chromosomes of each Ipomoea species. Duplication analysis indicated that segmental and tandem duplications contribute to expanding the Ipomoea MYB genes. Non-synonymous substitution (Ka) to synonymous substitution (Ks) [Ka/Ks] analysis showed that the duplicated Ipomoea MYB genes are mainly under purifying selection. Numerous cis-regulatory elements related to stress responses were detected in the MYB promoters. Six sweet potato transcriptome datasets referring to abiotic and biotic stresses were analyzed, and MYB different expression genes’ (DEGs’) responses to stress treatments were detected. Moreover, 10 sweet potato MYB DEGs were selected for qRT-PCR analysis. The results revealed that four responded to biotic stress (stem nematodes and Ceratocystis fimbriata pathogen infection) and six responded to the biotic stress (cold, drought, and salt). The results may provide new insights into the evolution of MYB genes in the Ipomoea genome and contribute to the future molecular breeding of sweet potatoes.

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Over-Expression of an R2R3 MYB Gene, MdMYB108L, Enhances Tolerance to Salt Stress in Transgenic Plants

Cited by 17 publications

References 63 publications

Mutation of MYB36 affects isoflavonoid metabolism, growth, and stress responses in Lotus japonicus

Mutation of MYB36 affects isoflavonoid metabolism, growth, and stress responses in Lotus japonicus

Physio-Biochemical Responses of Three Aquilegia Species Seedlings to Salt Stress

Comparative analysis of the MYB gene family in seven Ipomoea species

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