Over-Expression of AtPAP1 Transcriptional Factor Enhances Phenolic Acid Production in Transgenic Roots of Leonurus sibiricus L. and Their Biological Activities

Abstract

This study examines the production of five phenolic acids (chlorogenic acid, neochlorogenic acid, ferulic acid, caffeic acid and p-coumaric acid) following over-expression of AtPAP1 transcription factor by four transgenic root clones of Leonurus sibiricus after Agrobacterium rhizogenes transformation. The AtPAP1 expression level was estimated by quantitative real-time PCR. High levels of phenolic acids were found in the transgenic roots of L. sibiricus and were determined by high-performance liquid chromatography-mass spectrometry analysis. Additionally, transgenic roots showed antimicrobial potential and cytotoxic activity on glioma cells in IV grade. Our results suggest that L. sibiricus transformed roots with AtPAP1 gene over-expression may represent a potential source of phenolic acids.

An Extract of Transgenic Senna obtusifolia L. hairy roots with Overexpression of PgSS1 Gene in Combination with Chemotherapeutic Agent Induces Apoptosis in the Leukemia Cell Line

“…A number of examples of effective metabolic engineering strategies have been described so far [14]. For example, our previous studies show that overexpression of AtPAP1 (Arabidopsis thaliana the production of anthocyanin pigment 1 (PAP1) gene encoding an MYB transcription factor) increases production of phenolic compounds in hairy roots of Leonurus sibiricus L. with better biological activities in comparison to untransformed roots [19]. In turn Ni et al describe the enhancement of camptothecin production in Camptotheca acuminata hairy roots by overexpression of Octadecanoid-derivative Responsive Catharanthus AP2-domain gene (ORCA3).…”

Growth of Leonurus sibiricus L. roots with over-expression of AtPAP1 transcriptional factor in closed bioreactor, production of bioactive phenolic compounds and evaluation of their biological activity

“…It was kept dry and stored in the dark until further use. The yield (w/w) was 50.25% for the AtPAP1 extract (Sitarek et al, 2018).…”

“…The AtPAP1 root culture was established as described previously (Sitarek et al, 2018). The confirmation of the AtPAP1 root transgenic itself (Sitarek et al, 2018).…”

“…Moreover, they demonstrate rapid growth and promote the synthesis of phytochemicals whose biosynthesis requires differentiated cell types (Bannerjee et al, 2012). Our earlier studies revealed that transgenic roots of L. sibiricus with AtPAP1 transcriptional factor produce higher levels of phenolic acids than roots without any such construct; in addition, they showed better biological properties Sitarek et al, 2018). However, despite these successes, no commercial process currently exists for the production of phenolic acids by transgenic roots.…”

Transgenesis as a Tool for the Efficient Production of Selected Secondary Metabolites from Plant in Vitro Cultures

“…Other broad-spectrum metabolites can be modified to increase the production of specific molecules in in vitro plant cultures. For example, over-expression of the AtPAP1 transcriptional factor was found to enhance phenolic acid (such as chlorogenic acid, caffeic acid, ferulic acid or p-coumaric acid) production in a transgenic root culture of Leonurus sibiricus L., and the transgenic roots with the AtPAP1 transcriptional factor demonstrated better antimicrobial potential and cytotoxic activity against grade IV glioma cells [100]. Other studies have found such transgenic roots, incorporating the AtPAP1 transcriptional factor, to demonstrate better anticancer effects vis DNA damage, PARP cleavage/increased H2A.X histone levels and UHRF-1/DNMT1 downregulation of mRNA levels compared to untransformed roots [101].…”