Over-expression of chrysanthemum CmDREB6 enhanced tolerance of chrysanthemum to heat stress

Abstract

BackgroundChrysanthemum is among the top ten traditional flowers in China, and one of the four major cut flowers in the world, but the growth of chrysanthemum is severely restricted by high temperatures which retard growth and cause defects in flowers. DREB (dehydration-responsive element-binding) transcription factors play important roles in the response to abiotic and biotic stresses. However, whether the DREB A-6 subgroup is involved in heat tolerance has not been reported conclusively.ResultIn the present study, CmDREB6 was cloned from chrysanthemum (Chrysanthemum morifolium) ‘Jinba’. CmDREB6, containing a typical AP2/ERF domain, was classed into the DREB A-6 subgroup and shared highest homology with Cichorium intybus L. CiDREB6 (73%). CmDREB6 was expressed at its highest levels in the leaf. The CmDREB6 protein localized to the nucleus. Based on the yeast one hybrid assay, CmDREB6 showed transcription activation activity in yeast, and the transcriptional activation domain was located in the 3 ‘end ranging from 230 to 289 amino acids residues. CmDREB6 overexpression enhanced the tolerance of chrysanthemum to heat. The survival rate of two transgenic lines was as high as 85%, 50%, respectively, in contrast to 3.8% of wild-type (WT). Over-expression of CmDREB6 promoted the expression of CmHsfA4, CmHSP90, and the active oxygen scavenging genes CmSOD and CmCAT.ConclusionIn this study, DREB A-6 subgroup gene CmDREB6 was cloned from chrysanthemum ‘Jinba’. Overexpression of CmDREB6 enhanced heat tolerance of chrysanthemum by regulating genes involved in the heat shock response and ROS homeogenesis.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1400-8) contains supplementary material, which is available to authorized users.

Using of Nano - Selenium in Reducing the Negative Effects of High Temperature Stress on Chrysanthemum morifolium Ramat.

“…The production of chrysanthemum flowers is mainly controlled by the common growth factors as well as the environmental conditions in particular the stresses (e.g., salinity, drought and heat). The production of chrysanthemum flowers under heat stress has been reported by many researchers (e.g., Du et al 2018;Qi et al 2018;Kang et al 2019). It could be overcome the heat stress using the conventional and molecular breeding approaches (Li et al, 2016) for getting the desire quality of cut flowers and flowering of chrysanthemum in suitable time (Kang et al, 2019).…”

“…This acceleration in temperature is projected to increase 1.8 to 4°C at the end of this century with average increase by 0.2°C per decade (Zafar et al, 2018). Heat stress is considered one of the most important abiotic stresses, which causes serious problems for cultivated plants including the damage in photosynthesis process, plant enzymatic activities, and its metabolism (Haghighi et al, 2014 andDu et al, 2018). In general, cultivated plants may generate a heat shock response within a few hours, when the surrounding temperature get be higher (> 10-15 °C) to avoid this stress (Du et al, 2018).…”

“…lignans and phenolic glycosides (Yang et al, 2018 andPeng et al, 2019) as well as neuro-protective and antioxidant activity (Han et al, 2019. Chrysanthemum also is considered the four major cut flowers worldwide representing a great source in the global cut flower industry (Du et al, 2018 andDong et al, 2020). During all the year, this plant could be cultivated under different natural and artificial controlled environments (Kang et al, 2019).…”

“…The stressful environments in particular heat stress on chrysanthemum might cause a reduction in shelf life of plants resulted from delaying the flowering and deteriorating in the quality of flowers (Kang et al, 2019). The production of this plant was handled by many investigators under different stresses such as drought stress (Deng et al, 2012), water stress (Hodaei et al, 2018), salinity (Gao et al, 2018, heat stress (Du et al, 2018;Qi et al, 2018 andKang et al, 2019), UV-B radiation (Yang et al, 2018) and soil moisture regimes (Zhang et al, 2020). Chrysanthemum is severely restricted by heat stress due to its sensitivity to high temperatures, which mainly retards the plant growth causing defects in its flowers (Kang et al, 2019).…”

Peer Review #2 of "Identification and expression analysis of the DREB transcription factor family in pineapple (Ananas comosus (L.) Merr.) (v0.1)"

“…A novel A-5 subgroup gene from desert moss (Syntrichia caninervis), ScDREB8, enhanced the salt tolerance of Arabidopsis seedlings by up-regulating the expression of stress-related genes (Liang et al 2017). CmDREB6 belongs to the A-6 subgroup, and its overexpression enhanced the tolerance of chrysanthemum (Chrysanthemum morifolium) to heat stress (Du et al 2018). Pineapple (Ananas comosus (L.) Merr.…”