Over-expression of fibroblast growth factors in Xenopus embryos

Thompson, Jessica A. F.; Slack, Jonathan

doi:10.1016/0925-4773(92)90051-k

Cited by 26 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results are obtained with the human endometrial adenocarcinoma HEC-1-B cell line (Coltrini et al 1995). When introduced into Xenopus embryos, bFGF mRNA for a secretory form of bFGF has been shown to be 100-fold more potent in eliciting aberrant mesoderm formation than its native counterpart (Thompson and Slack 1992). Results from these studies indicate that the mechanism(s) of bFGF secretion exerts a critical control of the bioactivities of this multipotent growth factor.…”

mentioning

confidence: 72%

Ultrastructural Immunolocalization of Basic Fibroblast Growth Factor in Mast Cell Secretory Granules: Morphological Evidence for bFGF Release Through Degranulation

Kayton

Ahmadi

et al. 1998

J Histochem Cytochem.

View full text Add to dashboard Cite

SUMMARYWe previously reported that mast cells (MCs) serve as a source of basic fibroblast growth factor (bFGF), a potent angiogenic and mitogenic polypeptide, suggesting that bFGF may mediate MC-related neovascularization and fibroproliferation. Unlike many other growth factors, bFGF lacks a classic peptide sequence for its secretion, and the mechanism(s) for its release remains controversial. Because MCs release a wide spectrum of bioactive products via degranulation, we hypothesized that MC degranulation may be a mechanism of bFGF release and used ultrastructural immunohistochemistry to test the hypothesis. We reasoned that if bFGF is released through degranulation, it should be localized to MC secretory granules. Human tissues with chronic inflammation and rat/mouse tissues with anaphylaxis were studied. In all tissue samples examined, positive staining (or immunogold particle localization) for bFGF in MCs was predominantly in the cytoplasmic granules. Moderate bFGF immunoreactivity was also found in the nucleus, whereas the cytosol and other subcellular organelles exhibited minimal immunogold particle localization. In contrast, no immunogold particle localization for bFGF was observed in lymphocytes or plasma cells. In rat/mouse lingual tissue undergoing anaphylaxis, immunogold particle localization for bFGF was found not only in swollen cytoplasmic granules but also in the extruded granules of MCs. Three different anti-bFGF antibodies gave similar immunogold particle localization patterns, whereas all controls were negative. These results provide morphological evidence suggesting that, despite the lack of a classic secretory peptide in its structure, bFGF is localized to the secretory granules in MCs and may be released through degranulation.

show abstract

mentioning

confidence: 72%

Ultrastructural Immunolocalization of Basic Fibroblast Growth Factor in Mast Cell Secretory Granules: Morphological Evidence for bFGF Release Through Degranulation

Kayton

Ahmadi

et al. 1998

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…While nodal signaling is essential, FGF signaling also plays a crucial role in mesoderm formation, and an FGF ligand (FGF2) was the first identified mesoderm inducer (Kimelman and Kirschner, 1987;Slack et al, 1987Slack et al, , 1990 though since it lacks a signal sequence it may not be active in normal development (Thompson and Slack, 1992). Disruption of FGF signaling in the embryo with a dominant-negative receptor causes a loss of trunk and tail tissues (Amaya et al, 1991(Amaya et al, , 1993.…”

Section: Introductionmentioning

confidence: 99%

The role of FGF signaling in the establishment and maintenance of mesodermal gene expression in Xenopus

Fletcher

Harland

2008

Developmental Dynamics

View full text Add to dashboard Cite

FGF signaling is important for the formation of mesoderm in vertebrates, and when it is perturbed in Xenopus, most trunk and tail mesoderm fails to form. Here we have further dissected the activities of FGF in patterning the embryo by addressing its inductive and maintenance roles. We show that FGF signaling is necessary for the establishment of xbra expression in addition to its well-characterized role in maintaining xbra expression. The role of FGF signaling in organizer formation is not clear in Xenopus. We find that FGF signaling is essential for the initial specification of paraxial mesoderm but not for activation of several pan-mesodermal and most organizer genes; however, early FGF signaling is necessary for the maintenance of organizer gene expression into the neurula stage. Inhibition of FGF signaling prevents VegT activation of specific mesodermal transcripts. These findings illuminate how FGF signaling contributes to the establishment of distinct types of mesoderm.

show abstract

“…Briefly, mid-blastula stage animal cap explants are exposed to serial dilutions of the factor and cultured for up 3 days, after which they are analysed histologically or with molecular markers for mesoderm formation. The animal cap assay has also been extended to test the autoinducing activity of injected mRNAs, where it is assumed that the effective quantities of active proteins produced are proportional to the injected amounts of mRNA [19,20].…”

Section: The Animal Cap Assaymentioning

confidence: 99%

New perspectives on the role of the fibroblast growth factor family in amphibian development

Isaacs

1997

CMLS, Cell. mol. life sci.

View full text Add to dashboard Cite

It has been known for several years that the fibroblast growth factors (FGFs) have potent mesoderm-inducing activity. As a result they have been considered good candidates for one of the endogenous vegetally localized mesoderm-inducing signals in the amphibian Xenopus laevis. In this review the properties of the FGFs and their expression patterns in Xenopus are described. Recent work is discussed which reveals a close link between FGF signalling and regulation of the Xenopus brachyury (Xbra) gene. These data are used to build a model of FGF function which is quite different from what was originally conceived. Present evidence supports the view that during blastula stages the FGFs do not act as vegetally localized inducing signals. Instead, they are required in the animal hemisphere as competence factors, which provide a low level stimulation of the tyrosine kinase signal transduction pathway. FGF activity is necessary for the full range of responses to the vegetal inducing signals, including the activation of Xbra transcription in the marginal zone of the late blastula. Xbra is able to activate the zygotic transcription of eFGF, which suggests that there is a period of autocatalytic activation of eFGF and Xbra transcription within the forming mesoderm of the marginal zone. FGF activity continues to be required to maintain the expression of a sub-set of mesodermal genes, including Xbra, in the blastopore region and possibly also in the notochord through gastrula and neurula stages. In addition a role for the FGFs in anteroposterior specification and development of the myogenic lineages is discussed.

show abstract

Over-expression of fibroblast growth factors in Xenopus embryos

Cited by 26 publications

References 21 publications

Ultrastructural Immunolocalization of Basic Fibroblast Growth Factor in Mast Cell Secretory Granules: Morphological Evidence for bFGF Release Through Degranulation

Ultrastructural Immunolocalization of Basic Fibroblast Growth Factor in Mast Cell Secretory Granules: Morphological Evidence for bFGF Release Through Degranulation

The role of FGF signaling in the establishment and maintenance of mesodermal gene expression in Xenopus

New perspectives on the role of the fibroblast growth factor family in amphibian development

Contact Info

Product

Resources

About