Over-Expression of ICE1 Gene in Transgenic Rice Improves Cold Tolerance

Xiang, Dian-Jun; Hu, Xiangyang; Zhang, Yu; Yin, Kuide

doi:10.1016/s1672-6308(08)60039-6

Cited by 30 publications

(12 citation statements)

References 9 publications

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“…ICE1 is a constitutively expressed gene and post‐translation modification of its protein product, which is localized to the nucleus, is required for CBF induction. A similar mechanism is probably present in other species, because over‐expression of ICE1 in transgenic rice improves cold tolerance (Xiang, 2003), and ICE1 ‐like genes ( TaICE41 and TaICE87 ) that have been shown to bind the MYC elements in the promoters of certain CBF genes have been found in wheat (Badawi et al. , 2008).…”

Section: Introductionmentioning

confidence: 84%

Plant responses to cold: transcriptome analysis of wheat

Winfield

Wilson

et al. 2010

Plant Biotechnology Journal

263

193

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SummaryTemperature and light are important environmental stimuli that have a profound influence on the growth and development of plants. Wheat varieties can be divided on the basis of whether they require an extended period of cold to flower (vernalization). Varieties that have a requirement for vernalization also tend to be winter hardy and are able to withstand quite extreme subzero temperatures. This capacity, however, is not constitutive and plants require a period of exposure to low, non-freezing temperatures to acquire freezing tolerance: this process is referred to as cold acclimation. Cold acclimation and the acquisition of freezing tolerance require the orchestration of many different, seemingly disparate physiological and biochemical changes. These changes are, at least in part, mediated through the differential expression of many genes. Some of these genes code for effector molecules that participate directly to alleviate stress. Others code for proteins involved in signal transduction or transcription factors that control the expression of further banks of genes. In this review, we provide an overview of some of the main features of cold acclimation with particular focus on transcriptome reprogramming. In doing so, we highlight some of the important differences between cold-hardy and cold-sensitivevarieties. An understanding of these processes is of great potential importance because cold and freezing stress are major limiting factors for growing crop plants and periodically account for significant losses in plant productivity.

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Section: Introductionmentioning

confidence: 84%

Plant responses to cold: transcriptome analysis of wheat

Winfield

Wilson

et al. 2010

Plant Biotechnology Journal

263

193

View full text Add to dashboard Cite

show abstract

“…For instance, the expression levels of ICE1 and CBF3 can be enhanced in different freezing temperatures [40]. Ectopically expression of AtCBFs and AtICE1 in different plant species can enhance chilling tolerance [41,42], and ectopically expression of CBFs from other plants is able to enhance the freezing tolerance of transgenic Arabidopsis [20,43]. In the present work, it is shown that the transcription levels of VvICE1 and VvCBF3 were induced greatly and reached their peak at 7 h and 3 d, respectively, under low temperature in 'F-242' (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

et al. 2013

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Hydrogen sulfide (H 2 S) is an important signaling molecule involved in several stress-resistance processes in plants, such as drought and heavy metal stresses. However, little is known about the roles of H 2 S in responses to chilling stress. In this paper, we demonstrated that chilling stress enhance the H 2 S levels, the H 2 S synthetase (L-/D-cysteine desulfhydrase, L/DCD) activities, and the expression of L/DCD gene in Vitis vinifera L. 'F-242' . Furthermore, the seedlings were treated with sodium hydrosulfide (NaHS, a H 2 S donor) and hypotaurine (HT, a H 2 S scavenger) at 4°C to examine the effects of exogenous H 2 S on grape. The results revealed that the high activity of superoxide dismutase and enhanced expression of VvICE1 and VvCBF3 genes, but low level of superoxide anion radical, malondialdehyde content and cell membrane permeability were detected after addition of NaHS. In contrast, HT treatment displayed contrary effect under the chilling temperature. Taken together, these data suggested that H 2 S might be directly involved in the cold signal transduction pathway of grape.

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“…The transcripts bind to the CRT/DRE core elements of the cold-responsive gene promoter region and activate transcription of the COR gene, subsequently triggering a series of anti-cold physiological and biochemical reactions [6]. Studies have shown that overexpression of the Arabidopsis ICE1 gene in rice increases the cold tolerance of rice [7]. ICE1 is a constitutively expressed gene with different gene expression levels in different cold tolerance varieties; however, its differential expression regulatory mechanism is still unclear [8].…”

Section: Introductionmentioning

confidence: 99%

The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice

Guo

ShuXing

et al. 2019

IJMS

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Chilling stress is considered the major abiotic stress affecting the growth, development, and yield of rice. To understand the transcriptomic responses and methylation regulation of rice in response to chilling stress, we analyzed a cold-tolerant variety of rice (Oryza sativa L. cv. P427). The physiological properties, transcriptome, and methylation of cold-tolerant P427 seedlings under low-temperature stress (2–3 °C) were investigated. We found that P427 exhibited enhanced tolerance to low temperature, likely via increasing antioxidant enzyme activity and promoting the accumulation of abscisic acid (ABA). The Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq) data showed that the number of methylation-altered genes was highest in P427 (5496) and slightly lower in Nipponbare (Nip) and 9311 (4528 and 3341, respectively), and only 2.7% (292) of methylation genes were detected as common differentially methylated genes (DMGs) related to cold tolerance in the three varieties. Transcriptome analyses revealed that 1654 genes had specifically altered expression in P427 under cold stress. These genes mainly belonged to transcription factor families, such as Myeloblastosis (MYB), APETALA2/ethylene-responsive element binding proteins (AP2-EREBP), NAM-ATAF-CUC (NAC) and WRKY. Fifty-one genes showed simultaneous methylation and expression level changes. Quantitative RT-PCR (qRT-PCR) results showed that genes involved in the ICE (inducer of CBF expression)-CBF (C-repeat binding factor)—COR (cold-regulated) pathway were highly expressed under cold stress, including the WRKY genes. The homologous gene Os03g0610900 of the open stomatal 1 (OST1) in rice was obtained by evolutionary tree analysis. Methylation in Os03g0610900 gene promoter region decreased, and the expression level of Os03g0610900 increased, suggesting that cold stress may lead to demethylation and increased gene expression of Os03g0610900. The ICE-CBF-COR pathway plays a vital role in the cold tolerance of the rice cultivar P427. Overall, this study demonstrates the differences in methylation and gene expression levels of P427 in response to low-temperature stress, providing a foundation for further investigations of the relationship between environmental stress, DNA methylation, and gene expression in rice.

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Over-Expression of ICE1 Gene in Transgenic Rice Improves Cold Tolerance

Cited by 30 publications

References 9 publications

Plant responses to cold: transcriptome analysis of wheat

Plant responses to cold: transcriptome analysis of wheat

Hydrogen sulfide is involved in the chilling stress response in Vitis vinifera L.

The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice

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