Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco

Yao, Wenjing; Zhang, Dawei; Zhou, Boru; Wang, Jianping; Li, Renhua; Jiang, Tingbo

doi:10.1186/s12870-019-2191-2

Cited by 19 publications

(9 citation statements)

References 48 publications

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“…On the other hand, we found overexpression of the mlp gene, which has been recently related to increased drought tolerance and transcriptional level of abscisic acid synthetic genes in tobacco. The overexpression of mlp has also been correlated to reduced ROS accumulation and membrane damage during drought stress [ 95 ]. In addition, the edr gene, which encodes an enhanced disease response protein, was downregulated in the P. patens transcriptome (logFC = −2.17).…”

Section: Resultsmentioning

confidence: 99%

“…However, contradictorily, when plants recognize necrotrophic or hemibiotrophic pathogens, such as C. gloeosporioides , HR only contributes to faster spreading of the pathogen. Probably the transcriptional regulation of some genes involved in ROS alleviation in P. patens could be correlated with overexpression of mlp , which increased the expression of ROS-related genes (i.e., SOD) in tobacco during abiotic stress [ 95 ].…”

Section: Resultsmentioning

confidence: 99%

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Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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Anthracnose caused by the hemibiotroph fungus Colletotrichum gloeosporioides is a devastating plant disease with an extensive impact on plant productivity. The process of colonization and disease progression of C. gloeosporioides has been studied in a number of angiosperm crops. To better understand the evolution of the plant response to pathogens, the study of this complex interaction has been extended to bryophytes. The model moss Physcomitrium patens Hedw. B&S (former Physcomitrella patens) is sensitive to known bacterial and fungal phytopathogens, including C. gloeosporioides, which cause infection and cell death. P. patens responses to these microorganisms resemble that of the angiosperms. However, the molecular events during the interaction of P. patens and C. gloeosporioides have not been explored. In this work, we present a comprehensive approach using microscopy, phenomics and RNA-seq analysis to explore the defense response of P. patens to C. gloeosporioides. Microscopy analysis showed that appressoria are already formed at 24 h after inoculation (hai) and tissue colonization and cell death occur at 24 hai and is massive at 48 hai. Consequently, the phenomics analysis showed progressing browning of moss tissues and impaired photosynthesis from 24 to 48 hai. The transcriptomic analysis revealed that more than 1200 P. patens genes were differentially expressed in response to Colletotrichum infection. The analysis of differentially expressed gene function showed that the C. gloeosporioides infection led to a transcription reprogramming in P. patens that upregulated the genes related to pathogen recognition, secondary metabolism, cell wall reinforcement and regulation of gene expression. In accordance with the observed phenomics results, some photosynthesis and chloroplast-related genes were repressed, indicating that, under attack, P. patens changes its transcription from primary metabolism to defend itself from the pathogen.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Otero-Blanca

Pérez-Llano

Reboledo-Blanco

et al. 2021

JoF

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“…In other plant species, NAC TFs were also found to regulate cell wall thickening during fiber development, such as the NAC15 TF in Populus ( Zhong, Lee & Ye, 2010a ; Zhao et al, 2014 ). The PtrNAC15 gene overexpressed in transgenic tobacco plants played a significant role in wood formation ( Yao et al, 2020 ). In agreement with our results, the NAC13 gene of poplar ( Populus alba × P. glandulosa ) overexpressed in transgenic tobacco plants showed a positive regulation under salt stress resulting in a higher plant growth compared to control plants ( Zhang et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of theTectona grandis TgNAC01regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Hurtado

Oliveira

Gómez-Espinoza

et al. 2022

PeerJ

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NAC transcription factors play critical roles in xylem secondary development and in regulation of stress response in plants. NAC proteins related to secondary cell wall development were recently identified and characterized in Tectona grandis (teak), one of the hardwood trees of highest economic importance in the world. In this work, we characterized the novel TgNAC01 gene, which is involved in signaling pathways that mediate teak response to stress. Abscisic acid (ABA) increases TgNAC01 expression in teak plants. Therefore, this gene may have a role in signaling events that mediate ABA-dependent osmotic stress responsive in this plant species. Stable expression in tobacco plants showed that the TgNAC01 protein is localized in the cell nucleus. Overexpression of TgNAC01 in two out three independent transgenic tobacco lines resulted in increased growth, leaf senescence and salt tolerance compared to wild type (WT) plants. Moreover, the stress tolerance of transgenic plants was affected by levels of TgNAC01 gene expression. Water potential, gas exchange and chlorophyll fluorescence were used to determine salt stress tolerance. The 35S:TgNAC01-6 line under 300 mM NaCl stress responded with a significant increase in photosynthesis rate, stomatal conductance, transpiration and carboxylation efficiency, but lower water potential compared to WT plants. The data indicate that the TgNAC01 transcription factor acts as a transcriptional activator of the ABA-mediated regulation and induces leaf senescence.

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“…Overexpression of an OsNAC066 in rice improved drought and oxidative stress tolerance and increased ABA sensitivity [ 37 ]. In addition, 289 NAC TFs were retrieved and quantified based on RNA-seq datasets in our previous studies [ 38 – 40 ]. Based on log2 fold change (FC) ≥1 and false discovery rate (FDR) ≤0.05, 37 genes were significantly up-regulated under salt stress [ 40 ] and these genes could be vertically clustered into three groups.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco

et al. 2022

Self Cite

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Background Salt stress causes inhibition of plant growth and development, and always leads to an increasing threat to plant agriculture. Transcription factors regulate the expression of various genes for stress response and adaptation. It’s crucial to reveal the regulatory mechanisms of transcription factors in the response to salt stress. Results A salt-inducible NAC transcription factor gene PagNAC045 was isolated from Populus alba×P. glandulosa. The PagNAC045 had a high sequence similarity with NAC045 (Potri.007G099400.1) in P. trichocarpa, and they both contained the same conserved motifs 1 and 2, which constitute the highly conserved NAM domain at the N-terminus. Protein-protein interaction (PPI) prediction showed that PagNAC045 potentially interacts with many proteins involved in plant hormone signaling, DNA-binding and transcriptional regulation. The results of subcellular localization and transient expression in tobacco leaves confirmed the nuclear localization of PagNAC045. Yeast two-hybrid revealed that PagNAC045 protein exhibits transcriptional activation property and the activation domain located in its C-terminus. In addition, the 1063 bp promoter of PagNAC045 was able to drive GUS gene expression in the leaves and roots. In poplar leaves and roots, PagNAC045 expression increased significantly by salt and ABA treatments. Tobacco seedlings overexpressing PagNAC045 exhibited enhanced tolerance to NaCl and ABA compared to the wild-type (WT). Yeast one-hybrid assay demonstrated that a bHLH104-like transcription factor can bind to the promoter sequence of PagNAC045. Conclusion The PagNAC045 functions as positive regulator in plant responses to NaCl and ABA-mediated stresses.

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Over-expression of poplar NAC15 gene enhances wood formation in transgenic tobacco

Cited by 19 publications

References 48 publications

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Physcomitrium patens Infection by Colletotrichum gloeosporioides: Understanding the Fungal–Bryophyte Interaction by Microscopy, Phenomics and RNA Sequencing

Overexpression of theTectona grandis TgNAC01regulates growth, leaf senescence and confer salt stress tolerance in transgenic tobacco plants

Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco

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