Over-expression of the Brachypodium ASR gene, BdASR4, enhances drought tolerance in Brachypodium distachyon

Yoon, Jin Seok; Kim, Jae Yoon; Lee, Man Bo; Seo, Yong Weon

doi:10.1007/s00299-019-02429-7

Cited by 24 publications

(13 citation statements)

References 61 publications

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“…It has been proposed ASR1 gene expression increases under stress in a variety of species [55].The results of qRT-PCR analysis of OsASR1 and OsASR3 expression revealed that drought stress mainly regulates the expression of all ASR genes in rice and their over-expression in transgenic plants improved their drought and cold stress tolerance. Transgenic Brachypodium distachyon L. plants over-expressing BdASR4 gene retained more water and displayed higher tolerance to drought and antioxidant activities in comparison to the wild plants [56]. Physiological studies also proved the involvement of ASR1 gene in stress tolerance in transgenic tobacco, tomato, maize and rice [57].…”

Section: Plos Onementioning

confidence: 86%

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

et al. 2020

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Chickpea has a profound nutritional and economic value in vegetarian society. Continuous decline in chickpea productivity is attributed to insufficient genetic variability and different environmental stresses. Chickpea like several other legumes is highly susceptible to terminal drought stress. Multiple genes control drought tolerance and ASR gene plays a key role in regulating different plant stresses. The present study describes the molecular characterization and functional role of Abscissic acid and stress ripening (ASR) gene from chickpea (Cicer arietinum) and the gene sequence identified was submitted to NCBI Genbank (MK937569). Molecular analysis using MUSCLE software proved that the ASR nucleotide sequences in different legumes show variations at various positions though ASR genes are conserved in chickpea with only few variations. Sequence similarity of ASR gene to chickpea putative ABA/WDS induced protein mRNA clearly indicated its potential involvement in drought tolerance. Physiological screening and qRT-PCR results demonstrated increased ASR gene expression under drought stress possibly enabled genotypes to perform better under stress. Conserved domain search, protein structure analysis, prediction and validation, network analysis using Phyre2, Swiss-PDB viewer, ProSA and STRING analysis established the role of hypothetical ASR protein NP_001351739.1 in mediating drought responses. NP_001351739.1 might have enhanced the ASR gene activity as a transcription factor regulating drought stress tolerance in chickpea. This study could be useful in identification of new ASR genes that play a major role in drought tolerance and also develop functional markers for chickpea improvement.

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Section: Plos Onementioning

confidence: 86%

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

et al. 2020

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“…To identify the expression patterns of the genes, we carried out a qRT-PCR as previously described (Yoon et al 2019). Total RNA was extracted using TRIzol® reagent (Invitrogen).…”

Section: Gene Expression Studiesmentioning

confidence: 99%

Functional characterization of wheat histone H2B monoubiquitination enzyme TaHUB2 in response to vernalization in Keumkang (Triticum aestivum L.)

Kim

Khan

Kim

et al. 2021

Journal of Plant Interactions

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“…The three annual species of the genus have been selected as a model complex for polyploidy (diploids B. distachyon and B. stacei and derived allotetraploid B. hybridum; [30]) and one of its perennial species has been selected as a model species for perenniality (diploid B. sylvaticum; [31]). Transgenic plants of its flagship species B. distachyon have been analyzed to identify candidate genes that enhance drought stress tolerance in plants [32][33][34] though none of them specifically addressed dehydrins. Inspection of an early version of the B. distachyon reference genome Bd21 detected 36 LEA2 encoding genes but did not characterize the dehydrin genes [35].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses

Decena

Gálvez-Rojas

Agostini

et al. 2021

Plants

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Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachypodium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus’ flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.

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Over-expression of the Brachypodium ASR gene, BdASR4, enhances drought tolerance in Brachypodium distachyon

Cited by 24 publications

References 61 publications

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

Characterization of ASR gene and its role in drought tolerance in chickpea (Cicer arietinum L.)

Functional characterization of wheat histone H2B monoubiquitination enzyme TaHUB2 in response to vernalization in Keumkang (Triticum aestivum L.)

Comparative Genomics, Evolution, and Drought-Induced Expression of Dehydrin Genes in Model Brachypodium Grasses

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