Over expression of the P53 Oncoprotein in Carcinoma in Situ of the Testis

Kuczyk, Markus A.; Serth, Jürgen; Bokemeyer, Carsten; Allhoff, E.P.; Jonassen, Jens; Kuczyk, S.; Jonas, Udo

doi:10.1016/s0344-0338(11)80892-7

Cited by 12 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[76][77][78][79][80] Staining in a cytoplasmic membrane pattern is seen in IGCNU ( fig 15C) but not in normal spermatogonia, non-IGNCU atypical germ cells or in Sertoli cells. 76-78 80 The sensitivity of PLAP for this purpose varies from 83% to 100%.…”

Section: Intratubular Atypical Cellsmentioning

confidence: 99%

Morphological approach to tumours of the testis and paratestis

Emerson

Ulbright

2007

J Clin Pathol

View full text Add to dashboard Cite

Most neoplastic scrotal masses ultimately prove to be germ cell tumours and are recognisable with routine haematoxylin and eosin-stained sections. The differential diagnosis may be focused, even before reviewing histological sections, by knowledge of patient age, medical history, tumour site (testicular vs paratesticular) and gross findings. Some cases may prove to be diagnostically challenging, including rare tumours, a common tumour with an unusual pattern, a metastatic tumour, or a neoplasm with features that mimic another tumour. Several morphological patterns are seen with some frequency and these generate recurring sets of differential diagnostic considerations. These common patterns include testicular tumours with a predominant diffuse arrangement of cells with pale to clear cytoplasm, tumours with a glandular/tubular pattern, tumours with a microcystic pattern and tumours composed of oxyphilic cells. Intratubular proliferations of atypical cells, paratesticular glandular and/or papillary tumours, or tumours with spindle cell morphology can also be challenging to diagnose correctly. In some problematic cases, immunohistochemical staining may be useful to resolve these differential diagnoses.

show abstract

Section: Intratubular Atypical Cellsmentioning

confidence: 99%

Morphological approach to tumours of the testis and paratestis

Emerson

Ulbright

2007

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…PLAP, an isoenzyme of placental alkaline phosphatase, is the most commonly used immunohistochemical marker for IGCNU (7)(8)(9). Early IGCNU can be difficult to diagnose using only hematoxylin and eosin staining because, in the early stages, involved tubules may be few or contain a small number of neoplastic cells in an otherwise normal tubule.…”

Section: Discussionmentioning

confidence: 99%

“…Schenkman et al (21) and Guillou et al (17) found there was evidence of overexpression of wild-type p53 without mutation in GCTs of the testes. Numerous studies strongly suggest that intratubular germ cell neoplasia (IGCNU) is the precursor lesion to malignant germ cell tumors (9,22). Numerous studies strongly suggest that intratubular germ cell neoplasia (IGCNU) is the precursor lesion to malignant germ cell tumors (9,22).…”

Section: Discussionmentioning

confidence: 99%

Untitled

Moore¹,

Banner²,

Gökden³

et al. 2001

APPL. IMMUNOHISTOCHEM.

View full text Add to dashboard Cite

Intratubular germ cell neoplasia, unclassified (IGCNU) is a precursor of germ cell tumors (GCT) of the testis. In routine histologic sections, neoplastic intratubular germ cells may be very few and easily overlooked. The aim of this study is twofold: to establish the immunohistochemical pattern of expression of p53 in IGCNU and GCT and to determine whether p53 can be used as a marker for IGCNU. Resection specimens from 14 seminomas, 14 mixed germ cell tumors (MGCT), 3 embryonal carcinomas, 2 mature teratomas, 7 IGCNUs, and 11 normal testes were stained for p53. Normal germ cells and Sertoli cells of the seminiferous tubules in all normal testes were negative for p53. The tumor cells of all IGCNU cases were positive for p53. All invasive components of mixed germ cell tumors, embryonal carcinomas, and seminomas exhibited expression of p53. Mature teratoma components were negative for p53. These findings indicate that p53 is a highly sensitive marker of IGCNU and highly specific in distinguishing lesional tissue from normal seminiferous tubules. The current findings also suggest that p53 may be involved as an early step in the malignant progression of most germ cell neoplasias.

show abstract

“…In addition, the authors demonstrated that already 59% of intratubular malignant germ cells revealed a strong nuclear p53 reactivity, suggesting that p53 alterations might be involved in the pathogenesis of germ cell tumors. In the same context Kuczyk et al [27]found p53 overexpression in 6/8 intratubular malignant germ cells implicating p53 in the early development of germ cell tumors. Lewis et al [28]demonstrated nuclear p53 expression in 90% of seminomas and in 94% of nonseminomatous germ cell tumors (NSGCT) with a 98, 94 and 88% expression in clinical stage I, II and III disease.…”

Section: Tumor Suppressor Genesmentioning

confidence: 94%