Today, the vast majority of energy used in isolated systems is generated from fossil fuels. This paper assesses the potential for reducing CO2 emissions in two test systems, the Fernando de Noronha system and the platform supply vessel system using Energy storage. In addition, as a major contribution, a methodology is proposed to find the ideal and lowest cost combination of renewable energy generation to meet one of the project-specific criteria: renewable penetration level, percentage reduction of CO2 emissions and a combination of the two.The analysis in isolated island systems is performed using solar and wind energy, these renewable sources contribute to the reduction of fossil fuel dependence of these systems. The variability of these sources is attenuated with the use of energy storage systems, allowing the increase of the participation of renewable energies. The methodology applied to island power systems is based on the consideration of multiple scenarios with different rated photovoltaic power, wind turbines and energy storage systems. These scenarios are analyzed using the HOMER software.The results showed that the same level of renewable energy penetration can be achieved by multiple combinations of solar energy, wind energy and the energy stored in batteries. In addition, an economical assessment of the isolated system is shown, allowing to choose the most cost-effective combination that meets the desired penetration level, considering fuel price, renewables equipment, batteries, CO2 emissions tax and aging batteries. The methodology proposed in this paper allows the selection of a combination of renewable energy and batteries that can be made independently of the preliminary analysis, in which the solutions that meet the requirements are selected.Finally, it is shown that the use of batteries in diesel-powered ship power systems promotes a reduction in CO2 emissions. This analysis also shows the impact of varying different battery parameters and different Li-ion battery technologies such as Lithium Iron Phosphate and Lithium Titanate.