Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification

Dashti, Farshad; Ghahremani-Majd, Hojat; Esna-Ashari, M.

doi:10.1007/s11676-012-0314-9

Cited by 15 publications

(3 citation statements)

References 17 publications

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“…Sulphuric acid is thought to disrupt the seed coat and expose the lumens of macrosclereid cells, permitting imbibition of water which triggers germination (Nikoleave, 1977). The use of sulphuric acid in overcoming seed coat dormancy is well-known and several studies have indicated it as an effective agent to improve seed germination in hard seeded plant species (De Villiers, Van Rooyen, & Theron, 2002;Veassey & Teixeira De Freitas, 2002;Aliero, 2004;Usberti & Martins, 2007;Dashti, Ghahremani-Majd, & Esna-Ashari, 2012). Nondeep physiological dormancy is the most common form of dormancy found in seeds (Baskin & Baskin, 1998) and GA 3 is effective in breaking the nondeep physiological dormancy (Baskin & Baskin, 2004).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Seed Germination inTrema orientalis(L.) Blume—Potential Plant Species in Revegetation of Mine Wastelands

Rodrigues¹,

Rodrigues²

2013

Journal of Sustainable Forestry

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Natural forest succession takes a long time to accumulate sufficient nutrients to support plant growth and enhance soil microbial activity. Human intervention in selecting native pioneer plant species is therefore required to accelerate sustainable restoration. Trema orientalis (L.) Blume, a fast growing pioneer plant species, has the ability to grow in nutrient deficient soils and proves to have reclamation potential in mine wastelands. However, its use has been limited due to low germination percentages and nonsynchronous seed germination. In the present study we tested the effect of sulphuric acid (H 2 SO 4 ), hydrochloric acid (HCl), gibberellic acid (GA 3 ), and potassium nitrate (KNO 3 ) in varying concentrations and time durations on germination percentages and seed germination synchrony. We found that all treatments had a significant effect in predicting seed germination probabilities. Logistic regression analysis revealed that treatment solution and concentration had a significant effect on seed germination. Treatment with concentrated H 2 SO 4 for 15 min increased germination up to 92% within 20 days with the least imbibition time (8 days) and highest Seedling Vigor Index (491). The scanning electron microscope images of seeds treated with H 2 SO 4 showed complete dissolution of the honeycomb-like network of deposits on the seed coat surface removing the physical barrier and enhancing germination.

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Section: Discussionmentioning

confidence: 99%

Enhancement of Seed Germination inTrema orientalis(L.) Blume—Potential Plant Species in Revegetation of Mine Wastelands

Rodrigues¹,

Rodrigues²

2013

Journal of Sustainable Forestry

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“…Low seed germination speed and rate, slow seedling growth and low energy storage of seeds are some of the germination problems of some Allium species. Seed dormancy has been reported in a large number of wild Alliums, so it seems necessary to study methods to eliminate it in this species (Dashti et al 2012;Ebrahimi et al 2014;Phillips 2010). Seed and bulb dormancy in wild allium species is an adaptation to environmental conditions to avoid germination and growth in inappropriate seasons, so, in order to eliminate the dormancy of these organs, their natural environment should be modeled.…”

Section: Introductionmentioning

confidence: 99%

Optimizing proliferation and assessment of valak morpho-phenological traits; an endangered nutritious Allium endemic to Iran

Jafari

Hassandokht

Taheri

2021

Preprint

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Allium elburzense W. (subg. Melanocrommyum ) is an endangered and endemic species to Iran, which is called “valak” and known as a valuable and pricy vegetable with high nutritional and medicinal properties. This study was carried out in order to domestication, removing barriers of sexual reproduction, micropropagation and investigation of phenological stages and growth cycle of the species. The results indicated that fresh seeds had better germination than one year old seeds and appropriate germination temperature in A. elburzense W. seed was 12 °C. According to ANOVA results, the triple interaction of scarification (Sc), stratification period (StP) and GA 3 had a significant effect on seedling length, seedling fresh and dry weight, bulb height and diameter (p<0.01). Comparison of means showed that the treatments in which the StP (45 days) and scarification was applied had better effects on seed germination (66.5% germination) than other treatments. Murashige and Skoog with 300 mg L -1 KH2PO4 (MSP) medium caused a significant increase in the diameter of the basal plate compared to MS medium. Efficient system for in vitro propagation and conservation of valak genetic resources were the use of MSP culture medium supplemented with 0.6 mg l -1 NAA, 4 mg l -1 BA and 120 g l -1 of sucrose, which resulted in a direct propagation coefficient of 26.83 seedlings and bulblets at 18 weeks. As regards its life cycle, the juvenile phase in a low proportion of individuals (13.33%) lasted 2 years from seeding, while 87.66% of individuals were capable of producing inflorescence in the third year. Results showed that this plant is a metamorphosis and cross pollinator species. Results of present study can be used in domestication, conservation of genetic resources and proper exploitation of valak species.

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“…Conventionally, A. hirtifolium propagates through bulbs and seeds, but these two methods are not commercially efficient due to low growth rate of bulbs and deep dormancy of seeds (Dashti et al 2012;Ebrahimi et al 2014). The breeding process of Persian shallot due to low propagation rate is slow, and for that reason breeding on A. hirtifolium was rarely undertaken.…”

Section: Introductionmentioning

confidence: 99%

Haploid Induction via In Vitro Gynogenesis in Persian Shallot (Allium hirtifolium)

Panahandeh

Farhadi

2019

Journal of Horticultural Research

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Haploid induction using in vitro cultures of unpollinated flowers has been recognized as an important tool to produce homozygous plants for genetic studies and breeding programs. In this study the potential of gynogenic haploid induction in four ecotypes of Allium hirtifolium under different combinations of benzylaminopurine (BAP) with 2,4-dichlorophenoxyacetic acid (2,4-D), or α-naphthaleneacetic acid (NAA) was investigated. Unpollinated flower buds were excised from an umbel 5 to 3 days before anthesis, and cultured onto B5 medium containing 7.5% sucrose and 2 mg·dm−3 BAP with auxin. The experiments revealed that NAA increased the percentage of gynogenesis induction and number of gynogenic embryos per flower in all ecotypes. Somatic organogenesis from basal callus or other floral parts was most effective on the media containing 2,4-D. Plants obtained by gynogenesis were haploid in 70–77% and plants from somatic tissue were mostly diploid.

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Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification

Cited by 15 publications

References 17 publications

Enhancement of Seed Germination inTrema orientalis(L.) Blume—Potential Plant Species in Revegetation of Mine Wastelands

Enhancement of Seed Germination inTrema orientalis(L.) Blume—Potential Plant Species in Revegetation of Mine Wastelands

Optimizing proliferation and assessment of valak morpho-phenological traits; an endangered nutritious Allium endemic to Iran

Haploid Induction via In Vitro Gynogenesis in Persian Shallot (Allium hirtifolium)

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