2017
DOI: 10.1016/j.fsigen.2017.03.020
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Overcoming sodium dodecyl sulfate induced PCR inhibition

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Cited by 9 publications
(6 citation statements)
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“…To address this we instead removed dodecyl sulfate from the lysate with precipitation mediated by a potassium salt, leveraging the very low solubility of potassium dodecyl sulfate (KDS) in water. 39,40 We employed potassium chloride in the leading electrolyte in the ITP strip and in the reservoir. Upon application of the electric field, potassium cations migrated from the LE buffer toward the cathode in the TE reservoir.…”
Section: Rnase Detection Assaymentioning
confidence: 99%
“…To address this we instead removed dodecyl sulfate from the lysate with precipitation mediated by a potassium salt, leveraging the very low solubility of potassium dodecyl sulfate (KDS) in water. 39,40 We employed potassium chloride in the leading electrolyte in the ITP strip and in the reservoir. Upon application of the electric field, potassium cations migrated from the LE buffer toward the cathode in the TE reservoir.…”
Section: Rnase Detection Assaymentioning
confidence: 99%
“…Within this study, although a 2% v/v SDS solution exhibiting precipitation during extraction was used, it did not seem to negatively affect DNA purification. Due to the purification step within the extraction, also no inhibitory effects on the PCR caused by SDS or PBS could be determined in any of the samples, as already stated in other publications [65, 66]. Considering solely potential inhibitory effects when using other extraction or direct amplification methods, different moistening agents than SDS or PBS, for example, nonionic surfactants (i.e., Triton‐X‐100), might be advantageous.…”
Section: Discussionmentioning
confidence: 89%
“…52 Potassium phosphate may also be used to precipitate SDS out of solution so that PCR can tolerate up to 0.3% w/v SDS. 64 A notable study lysed bacterial cultures with 0.5% SDS and 200 mg/mL proteinase K. The resulting lysate was heated to 95 C to inactivate proteinase K and added directly to a PCR master mix containing Tween 20 for direct amplification and detection of target bacterial DNA. 52 This provides a helpful blueprint for how cumbersome RNA purification may be avoided when using SDS and proteinase K for lysis and RNase inactivation.…”
Section: Discussionmentioning
confidence: 99%