An oculomotor test is described that uses pupil diameter and eye movements during reading to detect deception. Forty participants read and responded to statements on a computerized questionnaire about their possible involvement in one of two mock crimes. Twenty guilty participants committed one of two mock crimes, and 20 innocent participants committed no crime. Guilty participants demonstrated speeded and accurate reading when they encountered statements about their crime and increases in pupil size. A discriminant function of oculomotor measures successfully discriminated between guilty and innocent participants and between the two groups of guilty participants. Results suggest that oculomotor tests may be of value for pre-employment and security screening applications.