Overexpressing hemp salt stress induced transcription factor genes enhances tobacco salt tolerance

Líu, Hao; Hu, Huaran; Tang, Kaixue; Rehman, Muzammal; Du, Guocheng; Huang, Yong; Liu, Feihu

doi:10.1016/j.indcrop.2021.114497

Cited by 8 publications

(3 citation statements)

References 45 publications

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“…It has been reported in previous studies that NAC TFs regulate the content of osmotic regulators in plants in response to salt stress. For example, tobacco plants overexpressing CsNAC1/2/3 gene accumulated higher levels of osmotically active substances than the control plants under salt stress (Liu et al, 2022), and transgenic plants overexpressing VuNAC1/2 exhibited higher Pro content and lower MDA content than the control plants under salt stress (Srivastava et al, 2022). In the present study, Pro content in the transgenic plants overexpressing LpNAC17 is significantly higher than in the controls under salt stress.…”

Section: Discussionsupporting

confidence: 43%

Lilium pumilum stress-responsive NAC transcription factor LpNAC17 enhances salt stress tolerance in tobacco

et al. 2022

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Lilium pumilum is a perennial herb with ornamental edible and medicinal value. It is an excellent wild germplasm resource with wide distribution and strong resistance. The NAC family of transcription factors is unique to higher plants. The NAC family plays a regulatory role in plant growth and development and participates in plant responses to biotic and abiotic stresses. The LpNAC17 gene of L. pumilum was cloned and transformed into tobacco to investigate the response of transgenic tobacco to salt stress. The results showed that the net photosynthetic rate and contents of chlorophyll in LpNAC17 over-expressed tobacco were higher than those in the control plants, while the stomatal conductance, transpiration rate and intercellular CO2 concentration were lower than those in the controls. The activity of superoxide dismutase, peroxidase, catalase, and the content of proline in LpNAC17 over-expressed tobacco were higher than those in the controls, while the content of malondialdehyde, superoxide anion, and hydrogen peroxide were lower than that in the control. Nitro-blue tetrazolium staining and 3,3′-diaminobenzidine tissue localization showed that the contents of O2- and H2O2 in transgenic tobacco was lower than in the controls. The expression levels of NtSOD, NtPOD, NtCAT, NtHAK1, NtPMA4, and NtSOS1 in the transgenic tobacco were higher than those in the controls. Therefore, this study provides a gene source for molecular breeding of salt-tolerant plants through genetic engineering, and lays a foundation for further research on salt-tolerant Lily.

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Section: Discussionsupporting

confidence: 43%

Lilium pumilum stress-responsive NAC transcription factor LpNAC17 enhances salt stress tolerance in tobacco

et al. 2022

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“…Salt tolerance of plants is a complex quantitative trait controlled by multiple genes [ 66 ]. During the response to salt stress, a large number of genes are activated, leading to the accumulation of many secondary metabolites involved in stress resistance, including trehalose, which are regulated by specific TFs [ 67 ]. At present, there are many studies on physiological changes of cultivated peanut under salt stress, but little attention has been paid to trehalose in the response of peanut to salt stress, and the research on its molecular mechanism is mostly focused on a few independent genes, so it is not easy to obtain systematic genetic information against salt stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and expression analysis of TPP gene family under salt stress in peanut (Arachis hypogaea L.)

Zhang,

Cao,

et al. 2024

PLoS ONE

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Trehalose-6-phosphate phosphatase (TPP), a key enzyme for trehalose biosynthesis in plants, plays a pivotal role in the growth and development of higher plants, as well as their adaptations to various abiotic stresses. Employing bioinformatics techniques, 45 TPP genes distributed across 17 chromosomes were identified with conserved Trehalose-PPase domains in the peanut genome, aiming to screen those involved in salt tolerance. Collinearity analysis showed that 22 TPP genes from peanut formed homologous gene pairs with 9 TPP genes from Arabidopsis and 31 TPP genes from soybean, respectively. Analysis of cis-acting elements in the promoters revealed the presence of multiple hormone- and abiotic stress-responsive elements in the promoter regions of AhTPPs. Expression pattern analysis showed that members of the TPP gene family in peanut responded significantly to various abiotic stresses, including low temperature, drought, and nitrogen deficiency, and exhibited certain tissue specificity. Salt stress significantly upregulated AhTPPs, with a higher number of responsive genes observed at the seedling stage compared to the podding stage. The intuitive physiological effect was reflected in the significantly higher accumulation of trehalose content in the leaves of plants under salt stress compared to the control. These findings indicate that the TPP gene family plays a crucial role in peanut’s response to abiotic stresses, laying the foundation for further functional studies and utilization of these genes.

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“…OsABA8ox1 -kd overexpressing seedlings had a higher level of ABA, reduced ROS contents, and Na + /K + ratio under salinity stress compared to non-transgenic plants ( Liu et al, 2022c ). Genetic engineering has achieved a landmark position in molecular breeding because of its role in developing several tolerant crop cultivars ( Li et al, 2022a ; Liu et al, 2022b ).…”

Section: Applications Of Genetic Engineering To Breed Salt-tolerant Ricementioning

confidence: 99%

Molecular tools, potential frontiers for enhancing salinity tolerance in rice: A critical review and future prospective

Rasheed

Nawaz

et al. 2022

Front. Plant Sci.

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Improvement of salinity tolerance in rice can minimize the stress-induced yield losses. Rice (Oryza sativa) is one of Asia’s most widely consumed crops, native to the subtropical regions, and is generally associated with sensitivity to salinity stress episodes. Salt-tolerant rice genotypes have been developed using conventional breeding methods; however, the success ratio is limited because of the complex nature of the trait and the high cost of development. The narrow genetic base of rice limited the success of conventional breeding methods. Hence, it is critical to launch the molecular tools for screening rice novel germplasm for salt-tolerant genes. In this regard, the latest molecular techniques like quantitative trait loci (QTL) mapping, genetic engineering (GE), transcription factors (TFs) analysis, and clustered regularly interspaced short palindromic repeats (CRISPR) are reliable for incorporating the salt tolerance in rice at the molecular level. Large-scale use of these potent genetic approaches leads to identifying and editing several genes/alleles, and QTL/genes are accountable for holding the genetic mechanism of salinity tolerance in rice. Continuous breeding practices resulted in a huge decline in rice genetic diversity, which is a great worry for global food security. However, molecular breeding tools are the only way to conserve genetic diversity by exploring wild germplasm for desired genes in salt tolerance breeding programs. In this review, we have compiled the logical evidences of successful applications of potent molecular tools for boosting salinity tolerance in rice, their limitations, and future prospects. This well-organized information would assist future researchers in understanding the genetic improvement of salinity tolerance in rice.

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Overexpressing hemp salt stress induced transcription factor genes enhances tobacco salt tolerance

Cited by 8 publications

References 45 publications

Lilium pumilum stress-responsive NAC transcription factor LpNAC17 enhances salt stress tolerance in tobacco

Lilium pumilum stress-responsive NAC transcription factor LpNAC17 enhances salt stress tolerance in tobacco

Genome-wide identification and expression analysis of TPP gene family under salt stress in peanut (Arachis hypogaea L.)

Molecular tools, potential frontiers for enhancing salinity tolerance in rice: A critical review and future prospective

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