Overexpressing of a novel wheat prolyl aminopeptidase gene enhances zinc stress tolerance in transgenic Arabidopsis thaliana

Wang, Yuange; Liu, Huaihua; Wang, Shuping; Li, Hongjie; Xin, Qingguo

doi:10.1007/s11240-015-0719-1

Cited by 18 publications

(7 citation statements)

References 35 publications

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“…The role of PAP homologues in plant abiotic stress tolerance has also been previously reported. Overexpression of Arabidopsis and wheat PAP1 in Arabidopsis enhanced resistance to drought and high salinity stresses including NaCl and ZnCl 2 , likely due to an increased accumulation of free proline (Sun et al, 2013;Wang et al, 2015a). The enhanced sensitivity of pap2 mutants to salt stresses and the corresponding lower accumulated proline presented here further supports the role of PAP2 in maintaining proline homeostasis in plants during stress.…”

Section: Discussionsupporting

confidence: 69%

“…Deletion of PAP1 in Arabidopsis decreased overall proline content and enhanced the plants susceptibility to osmotic stresses such as high salinity and drought (Sun et al, 2013), suggesting a role in maintaining proline homeostasis during stress. Similarly, overexpression of wheat PAP1 in Arabidopsis also renders an increased tolerance to zinc stress, which is also likely to be attributed to the increased proline level (Wang et al, 2015a). However, despite being identified to have a role in proline homeostasis, the molecular activity of PAP1 and its correct localisation remains to be confirmed.…”

Section: Discussionmentioning

confidence: 99%

“…Proline accumulation has been associated with tolerance to a range of abiotic stresses (Yoshiba et al, 1995;Choudhary et al, 2005). Additionally, PAP homologues in plants were previously identified to have a role in conferring tolerance to various abiotic stresses (Sun et al, 2013;Wang et al, 2015a). To investigate the potential role of PAP2 in the plant stress response, we examined pap2-1 and pap2-2 under salt and drought stress during vegetative growth (Figure 5a).…”

Section: Deletion Of Pap2 Enhances Plant Susceptibility To Abiotic Stmentioning

confidence: 99%

“…Overexpression of an Arabidopsis PAP1 isoform (At2g14260.2) resulted in an increase of accumulated free proline in leaf tissues, and a higher tolerance to drought and high salinity stresses (Sun et al, 2013). Similarly, overexpression of wheat (Triticum aestivum) PAP in Arabidopsis increased the level of accumulated proline in leaf that enhances survival rate and biomass of plants following zinc chloride (ZnCl 2 ) exposure (Wang et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

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A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

et al. 2020

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Amino acid recovery is the last step of organellar targeting peptide processing pathways, and prior studies have identified the metallo‐aminopeptidases responsible for the recovery of all amino acids from mitochondrial and chloroplastic targeting peptides, except for proline. This work characterises a proline‐specific aminopeptidase named proline aminopeptidase 2 (PAP2), identifying its activity, localisation and importance in maintaining proline homeostasis for normal plant development and during abiotic stress.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Deletion Of Pap2 Enhances Plant Susceptibility To Abiotic Stmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

et al. 2020

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“…Xanthi) has frequently been considered for phytoremediation purposes because of its high biomass and ability to take up and accumulate in leaves high amounts of metals, including zinc (Zn) ( Vangronsveld et al, 2009 ; Herzig et al, 2014 ; Vera-Estrella et al, 2017 ). To improve its capacity to take up and store metals in shoots, it has been transformed with a number of metal homeostasis genes, but with limited success ( Gisbert et al, 2003 ; Martínez et al, 2006 ; Gorinova et al, 2007 ; Wojas et al, 2008 , 2009 ; Korenkov et al, 2009 ; Siemianowski et al, 2011 ; Barabasz et al, 2013 ; Wang et al, 2015 ). Recently, it was shown that when expressing metal transporters to engineer new metal-related traits, a major part of the resulting phenotype was due to the modulation of endogenous gene expression ( Barabasz et al, 2016 ; Kendziorek et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Contribution of NtZIP1-Like to the Regulation of Zn Homeostasis

et al. 2018

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Tobacco has frequently been suggested as a candidate plant species for use in phytoremediation of metal contaminated soil but knowledge on the regulation of its metal-homeostasis is still in the infancy. To identify new tobacco metal transport genes that are involved in Zn homeostasis a bioinformatics study using the tobacco genome information together with expression analysis was performed. Ten new tobacco metal transport genes from the ZIP, NRAMP, MTP, and MRP/ABCC families were identified with expression levels in leaves that were modified by exposure to Zn excess. Following exposure to high Zn there was upregulation of NtZIP11-like, NtNRAMP3, three isoforms of NtMTP2, three MRP/ABCC genes (NtMRP5-like, NtMRP10-like, and NtMRP14 like) and downregulation of NtZIP1-like and NtZIP4. This suggests their involvement in several processes governing the response to Zn-related stress and in the efficiency of Zn accumulation (uptake, sequestration, and redistribution). Further detailed analysis of NtZIP1-like provided evidence that it is localized at the plasma membrane and is involved in Zn but not Fe and Cd transport. NtZIP1-like is expressed in the roots and shoots, and is regulated developmentally and in a tissue-specific manner. It is highly upregulated by Zn deficiency in the leaves and the root basal region but not in the root apical zone (region of maturation and absorption containing root hairs). Thus NtZIP1-like is unlikely to be responsible for Zn uptake by the root apical region but rather in the uptake by root cells within the already mature basal zone. It is downregulated by Zn excess suggesting it is involved in a mechanism to protect the root and leaf cells from accumulating excess Zn.

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Oilseed rape (Brassica napus): the importance of aminopeptidases in germination under normal and heavy metals stress conditions

2021

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BACKGROUND: Oilseed rape is one of the most important oilseed crops worldwide, crucial in the food and feed industries. Different environment and climatic conditions can influence its sustainable cultivation and crop yield. Aminopeptidases are crucial enzymes in many physiological processes in all organisms, including humans, so it is important to learn their behavior in food and feed sources. This study presents, for the first time, a detailed discussion on the importance of aminopeptidases, during the oilseed rape germination process, under standard and stress conditions. RESULTS: During the germination of oilseed rape under standard conditions, a significant increase in aminopeptidases activity toward N-terminal amino acidsphenylalanine (Phe), alanine (Ala), glycine (Gly), leucine (Leu), proline (Pro), methionine (Met) was observed. The change was substrate specific, with the highest increase being observed for Gly (3.2-fold), followed by Ala (2.9-fold), Pro (2.5-fold), Met (1.5-fold), and Phe (1.3-fold). Generally, N-terminal Phe was preferentially cleaved. Germination under stress conditions, caused by several heavy metal ions (e.g. divalent copper, zinc, cadmium, and lead ions), negatively influenced the plants' growth and quality, but significantly enhanced the expression of genes encoding aminopeptidases (or potentially activated aminopeptidases precursors), which was related to the dramatic increase of their activity.CONCLUSIONS: The activity/concentration of aminopeptidases in plants is adjusted to the needs at each stage of development and stress factors occurrence. The most significant increase of activity toward N-terminal Gly and Pro proved the key role of aminopeptidases in the defense mechanisms, by supplying the plants with osmoprotectants and organic nitrogen. The results provide new concepts of oilseed rape growth and cultivation under different conditions.

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Overexpressing of a novel wheat prolyl aminopeptidase gene enhances zinc stress tolerance in transgenic Arabidopsis thaliana

Cited by 18 publications

References 35 publications

A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

A mitochondrial prolyl aminopeptidase PAP2 releases N‐terminal proline and regulates proline homeostasis during stress response

Contribution of NtZIP1-Like to the Regulation of Zn Homeostasis

Oilseed rape (Brassica napus): the importance of aminopeptidases in germination under normal and heavy metals stress conditions

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